In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms

Ergül, Ali; Khabbazi, Saber Delpasand; Oğuz, Muhammet Çağrı; Özmen, Canan Yüksel; Gürel, Songül; Gürel, Ekrem

doi:10.1007/s11240-018-1410-0

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…In this study, the highest root formation rate was observed on medium supplemented with 1 mg/L IAA, while 2 mg/L IAA promoted root length better than 1 mg/L IAA. Our results on rooting efficiencies might differ slightly from other reports (Norouzi et al, 2005;Jafari et al, 2009;Ergül et al, 2018), presumably due to the composition of the media and genotypic differences.…”

Section: Discussioncontrasting

confidence: 99%

“…In this study, 1-1.5 mg/L BAP produced the highest regeneration rates. Similarly, Ritchie et al (1989), Mishutkina and Gaponenko (2006), and Ergül et al (2018) reported a significantly positive effect of 1-2 mg/L, 0.5-1 mg/L, and 1-3 mg/L BAP, respectively, on the regeneration rates of wild and cultivated sugar beet plants. Slightly different concentrations of BAP were found to be beneficial for regeneration rates in different studies, which might be due to the differences of the sugar beet genotypes used in each study.…”

Section: Discussionmentioning

confidence: 82%

“…In contrast, 15-20 weeks were required to regenerate plantlets through a callus phase from other explant types. The direct regeneration of transformed cells from cotyledonary node explants may prevent the somaclonal variation that was often observed in callus-based transformation methods (Hisano et al, 2004;Ergül et al, 2018). The availability of an efficient regeneration system enabled the testing of the effect of different sucrose concentrations on transformation efficiencies.…”

Section: Discussionmentioning

confidence: 99%

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Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta vulgaris L.)

Gürel¹,

Oğuz²,

Turan³

et al. 2019

Turk J Agric For

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Sugar beet (Beta vulgaris L.) is one of the most important industrial crops throughout world. With the availability of suitable genetic transformation technologies, the yield, quality, and stress tolerance of sugar beet could be improved significantly. However, low transformation efficiencies seriously limit the application of molecular technologies to the genetic improvement of sugar beet. With the aim of improving gene transfer techniques for sugar beet, the effect of different sucrose concentrations during cocultivation on the initial Agrobacterium-mediated transformation efficiencies in sugar beet was tested. To develop an efficient experimental system through which the effect of sucrose could be tested, first, a prolific regeneration system was optimized by testing the effect of different plant growth regulators on in vitro regeneration and rooting efficiencies from sugar beet cotyledonary node explants. The highest mean number of regenerated shoots per explant was obtained when the cotyledonary node explants excised from young seedlings were grown on MS medium supplemented with 1.0 mg/L 6-benzylaminopurine. Using this regeneration system, the effect of different concentrations of sucrose included in the cocultivation medium on the initial genetic transformation efficiencies observed in T 0 plants was tested using an Agrobacterium tumefaciens strain carrying the pBin19/35S:GUS-INT construct. The inclusion of 4.5% sucrose in the cocultivation medium resulted in significantly higher transformation (34.09%) and expression efficiencies (22.72%), confirmed by polymerase chain reaction and β-glucuronidase assays, respectively, in regenerated T 0 seedlings. If translated into stably inherited transformation efficiencies, these findings could contribute to the success of genetic transformation studies in sugar beet and other crops recalcitrant to Agrobacterium-mediated transformation.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta vulgaris L.)

Gürel¹,

Oğuz²,

Turan³

et al. 2019

Turk J Agric For

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“…In vitro multiplication based on culture of pre-existing meristems is promoted by adding cytokines to the culture medium in order to disrupt apical dominance and induce a repetitive axillary shoot growth [14,15,16,17,18,19,20,21,22]. The results of the multiplication stage evaluation showed that supplying BAP in the culture medium induced an increase in shoot multiplication rate for all cultivars compared to non-supplied treatments; effects previously reported in different plant species [22,23].…”

Section: Plant Adaptation To Ex Vitro Conditionsmentioning

confidence: 99%

Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

Padrón

Mercado

Abdala

et al. 2020

BSAA

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“…Regeneration frequency and genetic transformation of alfalfa have been studied by using different explants and growth regulators (Zhang et al, 2010;Kumar et al, 2012;Liu et al, 2013). Combination and concentration of growth regulators, photoperiod, pH, carbohydrates, and other growth supplements are among the determinative in vitro regeneration factors in different crops (Özcan et al, 1993;Özcan et al, 1996;Khabbazi et al, 2017;Ergül et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Improvement of the in vitro regeneration and Agrobacterium-mediated genetic transformation of Medicago sativa L.

Nofouzı¹,

Oğuz²,

Khabbazi³

et al. 2019

Turk J Agric For

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Alfalfa is a fodder crop that accounts for one of the best sources of protein and is widely cultivated around the world. In vitro regeneration of alfalfa has been studied earlier; however, most of the studies were almost intervened with callus formation. In this study, 3 explant sources (cotyledonary node, hypocotyl, and root crown) of two Turkish cultivars (Nimet and Savaş) were excised from young seedlings. Explants were subjected to different concentrations of BAP, BAP-IBA, and TDZ to evaluate the direct in vitro regeneration potential of selected plant parts. Moreover, we transformed the alfalfa plant with pBin19 harboring 35s.GUS-INT_35s.nptII construct to investigate the transformation efficiency and regeneration frequency after bacterial inoculation. The transformation was carried out by Agrobacterium tumefaciens strain GV2260. The highest mean number of regenerated shoots per explant was recorded as 8.5, 6.66, and 6.33 shoots per explant after cotyledonary node explants were treated with BAP (0.40 mg/L), BAP-IBA (1.25-0.06 mg/L), and TDZ (0.55 mg/L), respectively. The highest gene transformation frequency was 9.52% and 6.19% based on PCR and GUS assays. The regeneration frequency was decreased by up to 48.1% under kanamycin selection pressure. The effect of cultivar on shoot regeneration frequency, mean number of regenerated shoots, and gene transformation efficiency was significant. This study contributes to in vitro regeneration of alfalfa crop and its genetic transformation which could be utilized in future gene transformation studies.

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In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms

Cited by 8 publications

References 23 publications

Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta vulgaris L.)

Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta vulgaris L.)

Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

Improvement of the in vitro regeneration and Agrobacterium-mediated genetic transformation of Medicago sativa L.

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