In Vitro Mutagenesis and Genetic Improvement

Xu, Ling; Ullah, Najeeb; Naeem, Muhammad; Wan, G. L.; Jin, Ziqi; Khan, Fahim Halim; Zhou, Weijun

doi:10.1007/978-1-4614-0827-7_6

Cited by 17 publications

(6 citation statements)

References 90 publications

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“…Mutagenesis might generate novel genetic variations, yielding more effective genes than those that exist. One common practice is to do random mutagenesis followed by selection [72]. This can accelerate the selection process, thereby allowing for the analysis of larger ranges of genetic variation than are found in nature.…”

Section: Methods For Trait Improvementmentioning

confidence: 99%

Genomics of Entomopathogenic Nematodes and Implications for Pest Control

Baiocchi

Dillman

2016

Trends in Parasitology

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Entomopathogenic nematodes (EPNs) have been used in biological control but improvement is needed to realize their full potential for broader application in agriculture. Some improvements have been gained through selective breeding and the isolation of additional species and populations. Having genomic sequences for at least six EPNs opens the possibility of genetic improvement, either by facilitating the selection of candidate genes for hypothesis-driven studies of gene-trait relationships or by genomics-assisted breeding for desirable traits. However, the genomic data will be of limited use without a more mechanistic understanding of the genes underlying traits important for biological control. Additionally, molecular tools are required to fully translate the genomic resources into further functional studies and better biological control.

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Section: Methods For Trait Improvementmentioning

confidence: 99%

Genomics of Entomopathogenic Nematodes and Implications for Pest Control

Baiocchi

Dillman

2016

Trends in Parasitology

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“…Combinational use of in vitro culture and induced mutation is one of the suitable approaches to improvement of this important red banana [11,12] . Plant tissue culture provides direct shoot differentiation and number of in vitro plant cultures can be subjected to mutagenic treatments [13,14] . The success of any in vitro mutagenesis programme depends on the establishment of reproducible in vitro plant regeneration procedures, optimization of mutagenic treatments and efficient screening of the mutagenized populations for desired characters.…”

Section: Introductionmentioning

confidence: 99%

Improvement of red banana (Musa acuminata) through gamma ray: Determination of gamma ray dosage sensitivity and genetic exploration of dwarf traits through molecular marker

Sawardekar,

Sherkar,

Deshpande

et al. 2024

Int. J. Adv. Biochem. Res.

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Red banana is one of the favorite banana cultivar known for its taste and nutrition. Induced mutation technique is particularly important for bananas as there is limited sexual reproduction and parthenocarpic fruits. In this study, in vitro shoots of red banana were treated with gamma radiation ( 60 Cobalt). The objective was to determine the gamma ray dosage sensitivity (lethal dose-LD50) and identification of dwarf plants through molecular marker in red banana variety. The axenic cultures of passage 3-4 were irradiated with doses of 10,20,30, 40, 50, 60, 70, 80, 90 and 100 Gray (Gy). In vitro cultures were evaluated for percent survival, multiplication rate, length of shoots, number of leaves and length of leaves. Based on the values, the lethal-dose 50% (LD50) was determined as 20 Gy. Increasing levels of gamma irradiation doses more than 30 Gy, causes shoots turned to brown and simultaneously dead. Medium supplemented with 6 mg/l 6-benzylaminopurine (BAP) + 20 mg Casein hydrolysate + 30 mg/l Adenine sulphate showed maximum rate of in vitro multiplication rate (1:1.8) in irradiated cultures. Total 3,200 irradiated plants grown through micropropagation and their molecular analysis carried out through Sequence Characterized Amplified Regions (SCAR) markers. Molecular analysis revealed the presence of dwarf gene in 14, 18 and 52 sample plants as well as morphological study shows plant height 287cm , 296cm and 282cm, respectively.

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“…One of the frequently encountered problems in androgenetic research is the emergence of mixed ploidy plants due to a fusion of haploid nuclei in the early stages of androgenesis or endopolyploidy (Sangwan-Norreel, 1983). The use of mutation breeding through gamma rays together with anther culture to overcome these problems can be considered as an "ideal system" for plant breeding programs (Vagera et al, 1976;Maluszynski et al, 1995;Szarejko, 2003;Ahloowalia et al, 2004;Vagera et al, 2004;Arabi et al, 2005;Xu et al, 2012). Also, the integration of DH technology with existing biotechnological tools will increase genetic gains and breeding efficiency and ultimately lead to the rapid development of hybrids (Patial et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Androgenesis and Plant Regeneration From Wheat Anther Culture by Seed Pre-Sowing Gamma Irradiation

Bilgin

SARIER

Başer³

et al. 2022

Tekirdağ Ziraat Fakültesi Dergisi

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Combination breeding and mutation breeding are widely used methods in plant breeding. Intensive studies are carried out on biotechnological methods that will allow obtaining homozygous lines in a short time in populations obtained with these two techniques. Numerous studies have been carried out by different researchers on mutation breeding in wheat. There are few studies on anther culture response in mutagen-treated genotypes. Two different bread wheat promising advanced lines were used as the material in the study, in which the possibilities of combining mutation breeding with anther culture through ionizing radiation in high quality wheat breeding were investigated. In the study, the responses of advanced bread wheat mutant lines to anther culture, to which eight different doses of gamma rays (0, 100, 150, 200, 250, 300, 350, 400 Gy) were applied, including the control, were investigated. There are significant differences between genotypes and irradiation doses for all traits studied. It has been shown that it is possible to decrease albinism and increase the response of anther culture with dose-dependent gamma irradiation depending on bread wheat varieties. In the multiple comparison test to classify the difference between doses; statistically, 150 gray dose 5.60 is in the first statistical class and in the first place with the number of transferred green plants. After that, it ranks second in the same class with the number of green plants transferred with a 300 gray dose of 5.21. In the total number of regenerated green plants excluding controls (888), 635 unit (71.5%) and 205 unit (23.1%) haploids and spontaneous double haploid plants were obtained, respectively. In the study integrated into the bread wheat breeding program, a total of 205 spontaneous double haploid mutant lines were produced. According to the data obtained, it was shown that the gamma ray doses of 150 and 200 Gy had a significant stimulation effect on all parameters studied and ultimately the success index of anther culture in bread wheat compared to control.

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In Vitro Mutagenesis and Genetic Improvement

Cited by 17 publications

References 90 publications

Genomics of Entomopathogenic Nematodes and Implications for Pest Control

Genomics of Entomopathogenic Nematodes and Implications for Pest Control

Improvement of red banana (Musa acuminata) through gamma ray: Determination of gamma ray dosage sensitivity and genetic exploration of dwarf traits through molecular marker

Enhancement of Androgenesis and Plant Regeneration From Wheat Anther Culture by Seed Pre-Sowing Gamma Irradiation

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