In-Vitro Œstrogen Sensitivity of Breast-Cancer Tissue as a Possible Screening Method for Hormonal Treatment

Salih, H.; Flax, H.; Hobbs, J.R.

doi:10.1016/s0140-6736(72)90923-3

Cited by 41 publications

(9 citation statements)

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“…One other group (Flax et al, 1973;Salih, 1972) has previously published that the influence of hormones in vitro on breastcancer tissue, as measured by influence on glucose-6-phosphate dehydrogenase activity, was correlated with the clinical response in patients with advanced breast cancer. However, it should be pointed out that their method has never been reproduced (Masters et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

Short-term culture of human breast cancer: in vitro effects of hormones related to patient response

Poulsen¹,

Bichel²,

Andersen³

1982

Br J Cancer

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Summary.-Breast-cancer tissue from 60 patients was tested for oestrogen and testosterone sensitivity in vitro by measuring [3H]-dT incorporation in tissue fragments at various times during 48h culture. Hormone sensitivity in test culture was determined as an increase or decrease in dT uptake. In vitro cultures of breastcancer tissue demonstrate that some tumours are hormone-sensitive and others hormone-insensitive, but it cannot be predicted whether cell proliferation is stimulated or inhibited by hormone treatment.The data were related to the clinical stage of the patients, menopausal status, and the degree of anaplasia of the tumours tested. No correlation was observed between these parameters and in vitro hormonal sensitivity. However, when related to the response of patients to various kinds of hormonal treatment, a significant positive correlation was obtained.ONE THIRD OF PATIENTS with advanced breast cancer respond to endocrine therapy.The oestrogen-receptor (RE) assay on breast-cancer tissue has been used to select patients for hormonal treatment, but as present only half the patients with RE+ tumours actually benefit from endocrine therapy. It has been shown, however, that tumours containing both RE and progesterone receptors, and tumours containing high RE levels, are more likely to respond (70-80%) than tumours with low RE levels or none (5-10%; McGuire, 1980). Instead of measuring the initial binding step in the hormone action, another approach would be to determine hormonal influence on the tumours in vitro. It has been shown that the in vitro effect of hormones on breast-cancer tissue is not simply a reflection of the presence or absence of steroid receptors (Israel & Saez, 1978;Poulsen, 1978;Sanfillipo et al., 1979) and our proposed line of investigation might yield new information. Surgically excised breast-cancer tissue § To whom request for reprints should be addressed:

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Section: Discussionmentioning

confidence: 99%

Short-term culture of human breast cancer: in vitro effects of hormones related to patient response

Poulsen¹,

Bichel²,

Andersen³

1982

Br J Cancer

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“…Histochemistry and microchemistry.-The total pentose shunt dehydrogenase activity of the initial tumour material and after its culture was estimated semi-quantitatively by 2 different histochemical methods and quantitatively by a microchemical technique. The histochemical methods used were those either of Pearse (1972) with the monotetrazolium salt MTT (Sigma) as indicator, or of Salih's (1972aSalih's ( , b, 1973) modification of the Chayen et al (1970) technique using the ditetrazole NT (Sigma) as indicator. Phenazine methosulphate (BDH Ltd) was used as the hydrogen carrier from NADPH as recommended by Altmann' et al (1968), and polyvinyl alcohol (Bush, Beach and Segner Bailey) to stabilize the tissue sections during incubation (Altmann and Chayen, 1965;Altmann, 1971).…”

Section: Materials And) Methodsmentioning

confidence: 99%

“…For the most part, these have met with such limited success that their clinical use has not seemed justifiable. However, Salih, Flax and Hobbs (1972a;Salih et al, 1972bSalih et al, , 1973 have described a method based on the assessment of tumour cell enzyme activity in organ cultures, which purports to predict coiisistently in vivo breast tumour response to hormones.…”

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confidence: 99%

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An assessment of the effects of hormones on short term organ cultures of human breast carcinomata

Beeby¹,

Easty²,

Gazet³

et al. 1975

Br J Cancer

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Twenty-eight mammary carcinomata were maintained in organ culture in the presence of various hormones. The effects of the hormones have been assessed histologically by estimation of total dehydrogenases activity of the pentose glycolytic pathway and by the incorporation of tritiated thymidine or uridine into DNA or RNA. No significant effects on tumour cell activity due to hormones have been observed. Images Fig. 1 Fig. 2 Fig. 7 Fig. 8

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“…Theoretically, an individual patient's tumor could be utilized to determine hormonal behavior in vitro, and thus provide a guide for subsequent hor monal therapy. Previous in vitro studies [7,10,II] have demonstrated that some breast cancers preserve a viable histology and pre-incubation glucose 6-phosphate dehydrogenase activity only when incubated with specific hor mones, and by that definition are hormonally dependent. A similar study uti lizing 'H-thymidine incorporation by human breast cancer cell suspensions [5] has shown that increased incorporation can be demonstrated with specific hormonal supplementation of the incubation medium.…”

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confidence: 99%

Hormonally Enhanced Proliferation of Human Breast Cancer in Organ Culture

Lagios¹

1974

Oncology

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A method of short-term organ culture in chemically-defined media of human mammary carcinoma is described. Hormonally dependent proliferation over a 48-hour period is demonstrable in some human breast carcinomas, and uniform maintenance of the explants was obtained over the 48-hour period of incubation. The method permits routine explanting of an individual patient’s carcinoma in the organ culture system described, and may eventually provide useful information for management of advanced breast carcinoma with appropriate antihormonal therapy.

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In-Vitro Œstrogen Sensitivity of Breast-Cancer Tissue as a Possible Screening Method for Hormonal Treatment

Cited by 41 publications

References 5 publications

Short-term culture of human breast cancer: in vitro effects of hormones related to patient response

Short-term culture of human breast cancer: in vitro effects of hormones related to patient response

An assessment of the effects of hormones on short term organ cultures of human breast carcinomata

Hormonally Enhanced Proliferation of Human Breast Cancer in Organ Culture

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