In vitro organogenesis and plant regeneration of Thymus serpyllum L.: an important aromatic medicinal plant

Khajuria, Arun Kumar; Bisht, N. S.; Bhagat, Nisha

doi:10.1007/s11627-020-10094-9

Cited by 15 publications

(5 citation statements)

References 56 publications

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“…The genus Thymus L. is represented by around 220 described species in the world (Khajuria et al, 2020), and 36 subspecies (Kosakowska et al, 2020). Most of them have a great record of traditional uses as medicinal plants, spices, and aromatic species with interests in the industry of plant extracts due to their richness in volatile compounds (terpenes), which have been related to the antioxidant, antifungal, and antibacterial properties, among many others, of this plants' extracts (Ballester, 2016).…”

Section: Introductionmentioning

confidence: 99%

Thymol elicitation during in vitro regeneration of axillary bud explants from a Thymus piperella L. commercial hybrid

Juan-Vicedo

Juan-Méndez

Asensio-Casas

2022

Proceedings of MOL2NET'22, Conference on Molecular, Biomedical &Amp; Computational Sciences and Engineering, 8th Ed. - MOL2NET:

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Species of the genus Thymus L. (Lamiaceae) are of great interest as medicinal plants, as well as in the extracts and food technology industries due to the richness in bioactive specialized metabolites. Specifically, Iberian endemic species such as Th. piperella L., have a wide range of traditional uses in this sense. However, the micropropagation and biosynthesis of high-added value compounds under in vitro culture conditions has not been

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Section: Introductionmentioning

confidence: 99%

Thymol elicitation during in vitro regeneration of axillary bud explants from a Thymus piperella L. commercial hybrid

Juan-Vicedo

Juan-Méndez

Asensio-Casas

2022

Proceedings of MOL2NET'22, Conference on Molecular, Biomedical &Amp; Computational Sciences and Engineering, 8th Ed. - MOL2NET:

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“…VC has no appreciable negative or positive effects on regeneration (Figures S1-S6 E10b). VC is widely believed to function as an antioxidant (Mridula et al 2017;Lieber 2019;Khajuria et al 2020;Mishra et al 2020) but has also been known to be cytotoxic (Clément et al 2001). AC is often used as an additive which can reduce oxidative stress, absorb inhibitory compounds and toxic metabolites, thus promoting plant development (Thomas 2008).…”

Section: E7: Melatonin and Serotonin Combinationsmentioning

confidence: 99%

Factors affecting in vitro regeneration in the model tree Populus trichocarpa I. Medium, environment, and hormone controls on organogenesis

Goddard

Peremyslova

et al. 2022

In Vitro Cell.Dev.Biol.-Plant

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In preparation for a major GWAS (Genome Wide Association Study) of plant regeneration and transformation, a large number of factors were examined for their effects on indirect regeneration rate in diverse wild genotypes—seeking a high rate of regeneration, but also highly genetically variable and heritable treatments. Many of the factors examined have never before been reported on for their effects on callus, shoot, or root organogenesis in poplar (Populus). Stems had the highest regeneration potential, followed by petioles and leaves, with greenhouse grown explant sources superior to in vitro growth explant sources. Changes of ± 50% to Murashige and Skoog (MS) basal medium salts and micronutrients had a minor effect on regeneration. Many popular treatments that were evaluated also had little to no useful effect at the levels studied, including activated charcoal, ascorbic acid, silver nitrate, melatonin, serotonin, sucrose concentration, and lipoic acid. As a result of this wide exploration, treatment combinations that substantially elevated regeneration in diverse genotypes were identified, enabling GWAS.

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“…Литературные данные, касающиеся исследований по клональному микроразмножению тимьяна, довольно противоречивы. Большинство ученых для выделения первичных эксплантов используют асептические проростки, полученные из семян (Banna, 2017;Bekircan et al, 2018;Kulpa et al, 2019;Ansari et al, 2020;Khajuria et al, 2021), которые у перекрестно опыляемых видов представляют генетически неоднородный материал. Известны исследования, касающиеся размножения T. persicus (Roniger ex Reach.…”

Section: Introductionunclassified

“…максимальное количество побегов формировалось при использовании кинетина или аденина совместно с ГК 3. Некоторые исследователи получили высокий коэффициент размножения для разных видов тимьяна на среде, содержащей 0,5 мг/л кинетина и 2,0 мг/л БАП (Khajuria et al, 2021) или 5,0 мг/л изопентиладенина (2iр) (Kulpa et al, 2018). Замена БАП на 2iр способствовало повышению коэффициента размножения для T. × josephi-angeli J. Mansanet et Aguil.…”

Section: Introductionunclassified

Clonal micropropagation of some species of the genus Thymus L.

Yegorova,

Tevfik

2023

Turczaninowia

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The aim of the work was to study the influence of cultivation factors on the explants development to optimize the protocol of in vitro clonal micropropagation of four species of the genus Thymus L., valuable essential oil and medicinal plants. Conditions that ensure the production of up to 94.2 % of sterile explants were selected. It has been established that, when introduced into in vitro culture, it is advisable to use shoot tips and stem segments with a node (isolated from donor plants) and cultivate them in foil-covered test tubes. The optimal culture media for the first stage of micropropagation were determined: Murashige and Skoog (MS) medium with 1.0 mg/l benzylaminopurine (BAP) for Thymus serpyllum L. or MS with 1.0 mg/l kinetin and 1.0 mg/l gibberellic acids (GA3) for T. vulgaris L. ‘Gornyy Balzam’, T. × citriodorus (Pers.) Schreb. ‘Doone Valley’, T. caucasicus Willd. ex Ronniger. At the second stage of clonal micropropagation, it is advisable to cultivate T. caucasicus and T. serpyllum explants during 40 days, but T. vulgaris and T. × citriodorus – during 60 days. The maximum multiplication index at the recommended cultivation duration was obtained for T. × citriodorus (9.9) on MS medium without hormones, T. caucasicus (16.1) and T. vulgaris (10.0) – on MS medium with 1.0 mg/l kinetin., and T. serpyllum (6.8) on MS medium with 1.0 mg/l BAP. When comparing different culture vessels (tubes, jars and flasks), the effectiveness of jars was revealed. This type of culture vessels made it possible to increase the multiplication index up to 2.1–5.7 times. It was revealed that when microshoots of four thyme species were cultivated on MS medium with 1.0 mg/l IBA, the rooting rate reached 84.6–98.9 %. The frequency of adaptation ex vitro when using a mixture of peat and perlite (1:1) ranged from 62.8 to 89.5 %.

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In vitro organogenesis and plant regeneration of Thymus serpyllum L.: an important aromatic medicinal plant

Cited by 15 publications

References 56 publications

Thymol elicitation during <em>in vitro</em> regeneration of axillary bud explants from a <em>Thymus piperella </em>L. commercial hybrid

Thymol elicitation during <em>in vitro</em> regeneration of axillary bud explants from a <em>Thymus piperella </em>L. commercial hybrid

Factors affecting in vitro regeneration in the model tree Populus trichocarpa I. Medium, environment, and hormone controls on organogenesis

Clonal micropropagation of some species of the genus Thymus L.

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In vitro organogenesis and plant regeneration of Thymus serpyllum L.: an important aromatic medicinal plant

Cited by 15 publications

References 56 publications

Thymol elicitation during &lt;em&gt;in vitro&lt;/em&gt; regeneration of axillary bud explants from a &lt;em&gt;Thymus piperella &lt;/em&gt;L. commercial hybrid

Thymol elicitation during &lt;em&gt;in vitro&lt;/em&gt; regeneration of axillary bud explants from a &lt;em&gt;Thymus piperella &lt;/em&gt;L. commercial hybrid

Factors affecting in vitro regeneration in the model tree Populus trichocarpa I. Medium, environment, and hormone controls on organogenesis

Clonal micropropagation of some species of the genus Thymus L.

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Thymol elicitation during <em>in vitro</em> regeneration of axillary bud explants from a <em>Thymus piperella </em>L. commercial hybrid

Thymol elicitation during <em>in vitro</em> regeneration of axillary bud explants from a <em>Thymus piperella </em>L. commercial hybrid