In Vitro Osteogenic Potential of an Experimental Calcium Silicate–based Root Canal Sealer

Bryan, Thomas E.; Khechen, Khaled; Brackett, Martha Goël; Messer, Regina L. W.; El‐Awady, Ahmed; Primus, Carolyn M.; Gutmann, James L.; Tay, Franklin R.

doi:10.1016/j.joen.2010.03.034

Cited by 63 publications

(61 citation statements)

References 36 publications

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“…Because cytotoxic components can diffuse out of set hydraulic cements into periradicular tissues, both cement disks and their eluents were evaluated using a cycling regimen developed in our laboratory (27)(28)(29)(30). A weekly cycle consisted of direct evaluation of the toxicity of the cement disks over the plated cells for 3 days and indirect evaluation of the effect of eluents derived from the set cements on the plated cells.…”

Section: Cell Culturementioning

confidence: 99%

Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-like Cells

Wei¹,

Qi²,

Nikonov³

et al. 2012

Journal of Endodontics

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Section: Cell Culturementioning

confidence: 99%

Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-like Cells

Wei¹,

Qi²,

Nikonov³

et al. 2012

Journal of Endodontics

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“…Both materials are based on Portland cement, which has been shown to have low cytotoxicity. 19,20,36 Using normalized mitochondrial function as a measure, we found that PCS remained severely cytotoxic after the aging interval. This result implies that PCS continues to elute toxic levels of its components.…”

Section: Discussionmentioning

confidence: 94%

“…In 6-week tests, MTA and CS sealers are not lethal to osteoblasts (MC3T3), and appear to support osteoconduction, 19 although these materials also have some negative biological characteristics. MTA and CS may induce Cox-2, a mediator of pain, from primary cultured pulp cells, 20 or IL-1b, an important mediator of bone resorption, from mouse fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Dysregulation of monocytic cytokine secretion by endodontic sealers

et al. 2011

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Recent studies have reported that sealers may alter the secretion of specific cytokines from THP1 monocytic cells in vitro. In this study, a cytokine array was used to determine if endodontic sealers changed secretion of 42 cytokines. White mineral trioxide aggregate (WMTA), MTA preparation (CS), AH-Plus (AHP), and Pulp Canal Sealer (PCS) were mixed, allowed to set for 72 h, then "aged" in buffered-saline for 12 weeks. Aged specimens were placed in direct contact with THP1 for 72 h and their cytotoxicity (MTT assay) was assessed. Materials that were not severely toxic were then exposed to THP1 with or without lipolysaccharide (LPS), and the culture medium was assayed for cytokine secretion. Secretion of cytokines was quantified using infrared scanning (Odyssey(®)); replicate pairs were averaged. PCS severely suppressed MTT activity and was not assessed for its influence on cytokine secretion. WMTA, CS, and AHP induced a broad-based increase in cytokine secretion (>20% vs. Teflon controls), but AHP induced the greatest increase (>100% in 17 of 42 cytokines). The effects of the sealers on LPS-activated THP1 were biphasic, with some increases and decreases cytokine secretion of >20%, but few larger effects. This work shows endodontic sealers may alter the secretion of a broad cross section of cytokines from monocytic cells.

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“…However, although in vitro tests are faster and less expensive than in vivo tests, they do not comprehensively simulate in vivo conditions and the results obtained may not be relevant for the clinical setting 23) . In the case of MTT assay, Bryan et al 24) emphasized that it reduces undue discriminations as its test conditions clinically correspond to a classical filling.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay

et al. 2012

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The purpose of this study was to evaluate the cytotoxic effects of commercially available root canal sealers [Sealite Ultra (SU), Tubli-Seal (TS), Tubli-Seal EWT (TS-EWT), Pulp Canal Sealer (PCS), Pulp Canal Sealer EWT (PCS-EWT), Endomethasone N (En N), and Apexit Plus (AP)] on L929 cells by using MTT assay. After incubation with each sealer's extract at 37°C in a humidified air atmosphere containing 5% CO 2 for 24 h, MTT (5 mg/mL) in saline was added into each well and further incubated at 37°C for 4 h. Formazan precipitate was dissolved in a buffer containing 23% sodium dodecyl sulfate and 50% N,N-dimethylformamide (pH 4.7). Optical densities of dissolved formazan were read using a microplate spectrophotometer. AP, TS, and TS-EWT showed no cytotoxicity at any dilution tested. Other sealers exhibited some degree of cytotoxicity at the 1/4 and 1/2 dilutions. PCS-EWT and SU exerted more potent cytotoxicity at 1/2 dilution than the other sealers.

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In Vitro Osteogenic Potential of an Experimental Calcium Silicate–based Root Canal Sealer

Cited by 63 publications

References 36 publications

Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-like Cells

Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-like Cells

Dysregulation of monocytic cytokine secretion by endodontic sealers

Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay

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