2012
DOI: 10.1016/j.joen.2012.03.020
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Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-like Cells

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Cited by 51 publications
(47 citation statements)
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“…As a limitation, however, MTT assays may underestimate cellular damage and detect cell death only at the stage of apoptosis when cellular metabolism is substantially reduced (20). Our results showed similar cytotoxicity of experimental MTA-based material and Calen+ZO.…”
Section: Endodontic Filling Materials For Primary Teethsupporting
confidence: 59%
“…As a limitation, however, MTT assays may underestimate cellular damage and detect cell death only at the stage of apoptosis when cellular metabolism is substantially reduced (20). Our results showed similar cytotoxicity of experimental MTA-based material and Calen+ZO.…”
Section: Endodontic Filling Materials For Primary Teethsupporting
confidence: 59%
“…Flow cytometer analyzes the stained cells with different fluorescent characteristics at a single cell level and assesses the relative population viability at the end of the assay (33). Similar to a previous work (14), in the present study, the proportion of viable cells out of the total population of cells present in the cultures was measured and statistically compared.…”
Section: Discussionmentioning
confidence: 89%
“…The methyl-thiazoltetrazolium (MTT) colorimetric assay is widely used as a standard assay for evaluating the cytotoxicity of new biomaterials and has been routinely used to test dental and endodontic filling materials in cell culture systems (28)(29)(30). However, MTT assays may underestimate cellular damage and detect cell death only at the stage of apoptosis when cellular metabolism is substantially reduced (14); these assays have poor linearity with the cell number and low sensitivity to environmental conditions (31,32). To determine cell viability and cytotoxicity, alternative approaches have recently been proposed by using fluorescent dyes and flow cytometry (14), because this type of method is generally faster, less expensive, safer, and more sensitive to the cytotoxic events than alternative methods.…”
Section: Discussionmentioning
confidence: 99%
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