In Vitro Cytotoxicity Evaluation of a Novel Root Repair Material

Zhou, Huimin; Shen, Ya; Wang, Zhe-jun; Li, Li; Zheng, Yufeng; Häkkinen, Lari; Haapasalo, Markus

doi:10.1016/j.joen.2012.11.026

Cited by 164 publications

(152 citation statements)

References 33 publications

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“…Materials that have strong antibacterial properties also tends to have high cytotoxicity (9). The experimental MTA-based material in the present study showed a strong antibacterial effect, but also good low cytotoxicity.…”

Section: Endodontic Filling Materials For Primary Teethsupporting

confidence: 46%

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Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

et al. 2017

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This study assessed the pH, radiopacity, antimicrobial effect, cytotoxicity and biocompatibility of endodontic filling materials for primary teeth. Zinc oxide eugenol (ZOE), Vitapex and Calen paste thickened with zinc oxide (ZO) were evaluated in comparison to an experimental MTA-based material. Radiopacity was tested using a graduated aluminum stepwedge with a digital sensor (n=5). The materials pH was recorded at 1, 4, 12 h; 1, 3, 7, 15 and 30 days (n=5). Direct contact test was used to assess the antimicrobial efficacy against Enterococcus faecalis after 1, 4, 12, 24 h (n=5). Cytotoxicity assay used MTT test for cell viability after incubation for 1, 3 and 7 days (n=5). For biocompatibility test, Wistar rats had received implants containing each material (n=5). The biopsied tissues were histologically analyzed after 15, 30 and 60 days. The results of radiopacity, pH, antimicrobial capacity and cytotoxicity were analyzed using ANOVA and Tukey tests. The histological data were submitted to Kruskal-Wallis test. The experimental material presented the lowest radiopacity (3.28 mm Al) and had a pH>12.0 throughout the test period. The experimental material showed the highest antibacterial effect, killing over 99.97% bacteria in 4 h. Vitapex presented the highest cell viability. Initially, biocompatibility test showed moderate to severe inflammation in all groups. After 60 days, Calen+ZO group showed moderate inflammation, while the others showed predominantly mild inflammatory reaction. The present results demonstrated that the experimental MTA-based material exhibited satisfactory behavior regarding the studied properties. Additional in vivo studies are necessary for a better evaluation of the material. Physicochemical and Biological Evaluation of Endodontic Filling M a t e r i a l s f o r P r i m a r y Te e t h

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Section: Endodontic Filling Materials For Primary Teethsupporting

confidence: 46%

“…Human gingival fibroblasts were obtained from previously established cell line cultured from healthy patients who underwent oral surgery (9). Fibroblasts of the seventh to eighth passage were used in this study.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

et al. 2017

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“…It also has a better consistency after mixing and offers ease of placement. 23 Zhou et al 24 have stated that this material can be safely used in procedures requiring close approximation with the periodontal tissue. Two percent chlorhexidine was used as irrigant as it is placed to be more effective against Enterococcus faecalis than sodium hypochlorite.…”

Section: Discussionmentioning

confidence: 99%

External Inflammatory Root Resorption: Management of a Tooth with hopeless Prognosis

Shoba¹,

Abhilash²,

Balan³

et al. 2012

Conservative Dentistry and Endodontic Journal

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The treatment of external root resorption and associated periodontal defect can be challenging to the most ingenious clinician. A correct diagnosis and an understanding of the etiology and dynamics of root resorption, which is the progressive loss of dentin and cementum through action of osteoclastic cells, are critical for effective management. The article describes the management of an external root resorption in maxillary central incisor where a combined endo-perio management strategy was implemented. Cone beam computed tomography was used as an adjunctive diagnostic aid. A combined approach using biodentine for root surface repair, bone graft, collagen membrane, and platelet-rich fibrin to address the associated osseous lesion appears to be viable modality in treatment of the same. After a follow-up period of 12 months, the patient was found to be asymptomatic. Postoperative radiographs also demonstrated satisfactory bone fill and arrest of the resorptive lesion.

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“…Some in vitro methods are used to evaluate the toxicity of medical devices standardized by using cell cultures [37][38][39][40]. Methods involving cell cultures can be successfully employed because they are reproducible, fast, sensitive and easy to execute during an in vitro biocompatibility evaluation [41].…”

Section: Methods For In Vitro Cytotoxicity Analysesmentioning

confidence: 99%

Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products

Vidal¹,

Granjeiro²

2017

JBiSE

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The risks and damages related to the use of products, technologies and services of sanitary interest can be due to defects or manufacturing failures. Certain products already contain a certain degree of risk, which requires strict quality control in their production, distribution and use, as well as in the disposal of their waste in the environment. With continuous development in science and technology, medical devices must undergo intradermal irritation and testing for sensitization, cytotoxicity, and acute systemic toxicity. In health care, biotechnology aims to provide technology-based products or processes related to energy, food, and health, which are capable of stimulating new businesses, expanding exports, integrating the value chain and stimulating new demands for innovative products and processes, taking into account health policies. The present article was prepared by a bibliographical survey of the electronic databases PubMed, Lilacs, and Bireme. Cell culture testing can be successfully employed, as it is reproducible, rapid, sensitive, and financially accessible for performing in vitro toxicity testing. Thus, it has been possible to optimize the development phase of new products by decreasing animal use or even replacing them in certain tests. Some in vitro assays validated by the Organization for Economic Cooperation and Development in the area of health products have already replaced animal testing.

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In Vitro Cytotoxicity Evaluation of a Novel Root Repair Material

Cited by 164 publications

References 33 publications

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

Physicochemical and Biological Evaluation of Endodontic Filling Materials for Primary Teeth

External Inflammatory Root Resorption: Management of a Tooth with hopeless Prognosis

Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products

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