In vitro ovicidal and larvicidal activity of Agave sisalana Perr. (sisal) on gastrointestinal nematodes of goats

Abstract

This study describes the in vitro anthelmintic activity of aqueous extracts (AE), ethyl acetate extracts (EE), flavonoid fractions (FF) and saponin fractions (SF) obtained from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats. The activity of these extracts was evaluated by performing inhibition of egg hatch (EHA) and larval migration (LMI) assays. The EC(50) results of the EHA corresponded to 4.7, 0.1 and 0.05 mg/mL for EE, EA and FF, respectively. The SF fraction showed no ovicidal activity. The percent efficacies that were observed for the LMI were 50.3, 33.2 and 64.1% for the AE, EE and SF, respectively. The FF fraction did not show activity against the larvae. The analysis of the FF fraction indicates the presence of a homoisoflavonoid. This report suggests that the A. sisalana has activity in vitro against gastrointestinal nematodes of goats. This effect is likely related to the presence of homoisoflavonoid and saponin compounds, which have different actions for specific stages of nematode development.

Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

“…According to VERCRUYSSE et al (2001), a synthetic product is effective when it promotes anthelmintic activity above 90%. The mean value reported for the EC 90 (130.6μg mL -1 ) was, respectively, equal and higher to those reported by BOTURA et al (2013) for the ethyl acetate extract (130μg mL -1 ) and for flavonoid fraction (70µg mL -1 ) obtained in this same sisal residue extract. The authors attribute this ovicidal activity to the presence of homoisoflavonoids detected in the chemical analysis of flavonoid fraction (BOTURA et al, 2013).…”

“…The mean value reported for the EC 90 (130.6μg mL -1 ) was, respectively, equal and higher to those reported by BOTURA et al (2013) for the ethyl acetate extract (130μg mL -1 ) and for flavonoid fraction (70µg mL -1 ) obtained in this same sisal residue extract. The authors attribute this ovicidal activity to the presence of homoisoflavonoids detected in the chemical analysis of flavonoid fraction (BOTURA et al, 2013). Bearing in mind that the succinic acid used in this study was also obtained from the ethyl acetate extract from the sisal liquid residue, the results suggested a possible synergistic or additive action of the SA with these flavonoids.…”

“…The succinic acid had no effect on larval motility at the concentration used. BOTURA et al (2013) reported moderate larvicidal activity of the saponin fraction from the sisal waste (efficacy of 64.1%). These results suggested the participation of one more chemical component in the anthelmintic activity of A. sisalana.…”

“…Our research group has investigated the biological activity of different extracts and fractions obtained from sisal waste against nematodes of goats. In the context, the flavonoid and saponin fractions from ethyl acetate extract showed ovicidal and larvicidal effects, respectively (SILVEIRA et al, 2012;BOTURA et. al., 2013;SANTOS et al, 2015).…”

Anthelmintic Activity of Fruit Extract and Fractions of Piper longum L. In vitro

“…present in the extracts and fractions of P. longum contributed to the anthelmintic potency of the fruits. 27 Though the exact mechanism of how these plant chemicals because anthelmintic activity has not been established, certain hypothesis had been predicted. Destabilization of egg membranes led to penetration of plant extract into the egg and caused either embryonic mortality or prevented development of embryo within the egg.…”