This study describes the in vitro anthelmintic activity of aqueous extracts (AE), ethyl acetate extracts (EE), flavonoid fractions (FF) and saponin fractions (SF) obtained from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats. The activity of these extracts was evaluated by performing inhibition of egg hatch (EHA) and larval migration (LMI) assays. The EC(50) results of the EHA corresponded to 4.7, 0.1 and 0.05 mg/mL for EE, EA and FF, respectively. The SF fraction showed no ovicidal activity. The percent efficacies that were observed for the LMI were 50.3, 33.2 and 64.1% for the AE, EE and SF, respectively. The FF fraction did not show activity against the larvae. The analysis of the FF fraction indicates the presence of a homoisoflavonoid. This report suggests that the A. sisalana has activity in vitro against gastrointestinal nematodes of goats. This effect is likely related to the presence of homoisoflavonoid and saponin compounds, which have different actions for specific stages of nematode development.

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Agave sisalana (sisal) is known worldwide as a source of hard fibers, and Brazil is the largest producer of sisal. Nonetheless, the process of removing the fibers of the sisal leaf generates 95% waste. In this study, we applied chemical sequential steps (hydrothermal extraction, precipitation, liquid-liquid extraction, crystallization, SiO2 and Sephadex LH 20 column chromatography) to obtain pectin, mannitol, succinic acid, kaempferol and a mixture of saponins as raw chemicals from sisal biomass. The structural identification of these compounds was performed though spectrometric methods, such as Infrared (IR), Ultraviolet (UV), Mass spectrometry (MS) and Nuclear magnetic resonance (NMR). All the sisal chemicals found in this work are used by both the chemical and pharmaceutical industries as excipients or active principles in products.

Context: The concept of code smells is widespread in Software Engineering. However, in spite of the many discussions and claims about them, there are few empirical studies to support or contest these ideas. In particular, the study of the human perception of what is a code smell and how to deal with it has been mostly neglected. Objective: To build empirical support to understand the effect of god classes, one of the most known code smells. In particular, this paper focuses on how conceptualization affects identification of god classes, i.e., how different people perceive the god class concept. Method: A controlled experiment that extends and builds upon another empirical study about how humans detect god classes [19]. Our study: i) deepens and details some of the research questions of the previous study, ii) introduces a new research question and, iii) when possible, compares the results of both studies. Result: Our findings show that participants have different person al criteria and preferences in choosing drivers to identify god classes. The agreement between participants is not high, which is in accordance with previous studies. Conclusion: This study contributes to expand the empirical data about the human perception of code smells. It also presents a new way to evaluate effort and distraction in experiments through the use of automatic logging of participant actions

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