In vitro phagocytosis and monocyte-macrophage activation with poly(lactide) and poly(lactide-co-glycolide) microspheres

Prior, Sandra; Gander, Bruno; Blarer, Natalia; Merkle, Hans P.; Subirá, Marija Luisa; Irache, Juan M.; Gamazo, Carlos

doi:10.1016/s0928-0987(01)00218-4

Cited by 126 publications

(70 citation statements)

References 27 publications

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“…This is a widely used method to recognize the surface charges of NPs (Ratner et al, 1987;Soppimath et al, 2001). Hydrophobicity and hydrophilicity determined by Water contact angle measurements and hydrophobic interaction chromatography respectively (Storm et al, 1995;Manuela Gaspar et al, 1998;Mosqueira et al, 2001;Prior et al, 2002;Avgoustakis et al, 2002;. There are many sensitive methods for characterizing nanoparticles, depending upon the factor being investigated (Dinarvand et al, 2011).…”

Section: Nanoparticle Characterization Techniquesmentioning

confidence: 99%

PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

Mirakabad

Nejati-Koshki²,

Akbarzadeh³

et al. 2014

Asian Pacific Journal of Cancer Prevention

406

154

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Section: Nanoparticle Characterization Techniquesmentioning

confidence: 99%

PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

Mirakabad

Nejati-Koshki²,

Akbarzadeh³

et al. 2014

Asian Pacific Journal of Cancer Prevention

406

154

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“…Size exclusion chromatography [58][59][60] Surface chemistry analysis X-ray photoelectron spectroscopy 28,61,62 Fourier transform infrared spectroscopy 54,63,64 Nuclear magnetic resonance spectroscopy [64][65][66] Surface charge Zetasizer 56,67,68 Crystallinity X-ray diffraction 37,69,70 Differential scanning calorimetry [71][72][73] Hydrophobicity, hydrophilicity water contact angle measurements 65,74,75 Hydrophobic interaction chromatography [76][77][78] Drug entrapment efficiency, drug release studies…”

Section: Molecular Weightmentioning

confidence: 99%

Polylactide-co-glycolide nanoparticles for controlled delivery of anticancer agents

Dinarvand¹,

Sepehri²,

Manoochehri³

et al. 2011

IJN

396

265

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The effectiveness of anticancer agents may be hindered by low solubility in water, poor permeability, and high efflux from cells. Nanomaterials have been used to enable drug delivery with lower toxicity to healthy cells and enhanced drug delivery to tumor cells. Different nanoparticles have been developed using different polymers with or without surface modification to target tumor cells both passively and/or actively. Polylactide-co-glycolide (PLGA), a biodegradable polyester approved for human use, has been used extensively. Here we report on recent developments concerning PLGA nanoparticles prepared for cancer treatment. We review the methods used for the preparation and characterization of PLGA nanoparticles and their applications in the delivery of a number of active agents. Increasing experience in the field of preparation, characterization, and in vivo application of PLGA nanoparticles has provided the necessary momentum for promising future use of these agents in cancer treatment, with higher efficacy and fewer side effects.

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“…In addition, phagocytosed microparticles release encapsulated antigen into the cells where it is efficiently presented on major histocompatibility complex (MHC) I and MHC II (15). Moreover, phagocytosed PLGA and PLA microparticles have been found to activate monocytes in vitro (16). Recently, uptake of PLGA nanoparticles has also been demonstrated to induce stimulation of DCs (17).…”

Section: Introductionmentioning

confidence: 99%

Composition and Surface Charge of DNA-Loaded Microparticles Determine Maturation and Cytokine Secretion in Human Dendritic Cells

Jilek¹,

Ulrich²,

Merkle³

et al. 2004

Pharm Res

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Purpose. Biodegradable microparticles prepared from poly(lactide) (PLA) and poly(lactide-co-glycolide) (PLGA) have been shown to be promising carrier systems for vaccine delivery. Here, we have investigated the capacity of different PLA and PLGA microparticle formulations to induce stimulation of human blood monocyte-derived dendritic cells (DCs). Methods. Stimulation of human derived dendritic cells by plain microparticles were compared with microparticles loaded with plasmid DNA or double-stranded salmon DNA either by encapsulation or adsorption to the surface of cationic microparticles. Stimulation of DCs was monitored by the up-regulation of surface maturation markers CD83 and CD86 and the secretion of IL-12 and TNF-␣. Results. Slowly degrading PLA microparticles did not induce any detectable stimulation or activation of DCs. In contrast, fast degrading PLGA microparticles were able to influence DC maturation and cytokine secretion dependent on their surface charge. Anionic PLGA microparticles induced an up-regulation of CD83 and high TNF-␣ secretion, which was further enhanced up to the level of the potent stimulator lipopolysaccharide (LPS) when plasmid DNA was encapsulated. Moreover, the secretion of significant amounts of IL-12 was observed. Cationic PLGA microparticles induced an up-regulation of CD86 and moderate TNF-␣ secretion, but no IL-12 secretion, with no additional effects in the presence of plasmid DNA. Conclusions. The data suggest that the composition and charge of biodegradable DNA-loaded microparticles profoundly influences maturation and cytokine secretion in DCs. Thus, the individual formulation of microparticles used as a vaccine carrier system might considerably influence the profile of the immune response.

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In vitro phagocytosis and monocyte-macrophage activation with poly(lactide) and poly(lactide-co-glycolide) microspheres

Cited by 126 publications

References 27 publications

PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

Polylactide-co-glycolide nanoparticles for controlled delivery of anticancer agents

Composition and Surface Charge of DNA-Loaded Microparticles Determine Maturation and Cytokine Secretion in Human Dendritic Cells

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