PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

Mirakabad, Fatemeh Sadat Tabatabaei; Nejati-Koshki, Kazem; Akbarzadeh, Abolfazl; Yamchi, Mohammad Rahmati; Milani, Morteza; Zarghami, Nosratollah; Zeighamian, Vahideh; Rahimzadeh, Amirbahman; Alimohammadi, Somayeh; Hanifehpour, Younes; Joo, Sang Woo

doi:10.7314/apjcp.2014.15.2.517

Cited by 406 publications

(168 citation statements)

References 224 publications

(322 reference statements)

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“…Nanoparticles can be coated with molecules that conceal the hydrophobicity by providing a hydrophilic layer at the surface. The most general method (Sadat Tabatabaei Mirakabad et al 2014b) for surface modification is use of the hydrophilic and non-ionic polymer polyethylene glycol (PEG) (Danhier et al 2012, Sadat Tabatabaei Mirakabad et al 2014b. The existence of PEG on the PLGA nanoparticles imparts extra functionality through the use of polymeric NPs (Mahapatro and Singh 2011).…”

Section: Introductionmentioning

confidence: 99%

A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line

Mirakabad

Akbarzadeh

Milani

et al. 2014

Artificial Cells, Nanomedicine, and Biotechnology

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Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death among women worldwide. Herbal medicines have tremendous potential as promising agents for the treatment of cancer. Curcumin is a natural polyphenol which has many anticancer effects. Because of its low aqueous solubility, low bioavailability, and quick degradation and metabolism, curcumin was released using PLGA-PEG nanoparticles. Herein, the efficiency of pure curcumin and curcumin-loaded PLGA-PEG in MCF-7 human breast cancer cell lines was studied. (1)H NMR, FT-IR and SEM demonstrated PLGA-PEG structure and curcumin loaded on nanoparticles. Subsequently, the cytotoxic effects of free curcumin and curcumin-loaded PLGA-PEG were determined via an MTT assay. Our study confirmed that curcumin-loaded PLGA-PEG has more cytotoxic effects on the MCF-7 breast cancer cell line and could be exploited as a potential source for developing novel drugs against breast cancer.

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Section: Introductionmentioning

confidence: 99%

A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line

Mirakabad

Akbarzadeh

Milani

et al. 2014

Artificial Cells, Nanomedicine, and Biotechnology

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“…Polymeric NP have been considered as the most promising system for ocular drug delivery [11,12]. Owing to its excellent biocompatibility, biodegradability, and mechanical strength, poly(D,L-lactide-co-glycolide) (PLGA) has been employed for the delivery of therapeutic agents [13]. It was selected as a matrix to prepare small NP with size <300 nm for ocular administration [14].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

et al. 2015

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Abstract. This study was conducted to develop formulations of hydrocortisone butyrate (HB)-loaded poly(D,L-lactic-co-glycolic acid) nanoparticles (PLGA NP) suspended in thermosensitive gel to improve ocular bioavailability of HB for the treatment of bacterial corneal keratitis. PLGA NP with different surfactants such as polyvinyl alcohol (PVA), pluronic F-108, and chitosan were prepared using oil-in-water (O/W) emulsion evaporation technique. NP were characterized with respect to particle size, entrapment efficiency, polydispersity, drug loading, surface morphology, zeta potential, and crystallinity. In vitro release of HB from NP showed a biphasic release pattern with an initial burst phase followed by a sustained phase. Such burst effect was completely eliminated when nanoparticles were suspended in thermosensitive gels and zero-order release kinetics was observed. In HCEC cell line, chitosanemulsified NP showed the highest cellular uptake efficiency over PVA-and pluronic-emulsified NP (59.09±6.21%, 55.74±6.26%, and 62.54±3.30%, respectively) after 4 h. However, chitosan-emulsified NP indicated significant cytotoxicity of 200 and 500 μg/mL after 48 h, while PVA-and pluronic-emulsified NP exhibited no significant cytotoxicity. PLGA NP dispersed in thermosensitive gels can be considered as a promising drug delivery system for the treatment of anterior eye diseases.

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“…ϩ DCs-The human CD8 ϩ T cell receptor transgenic cell line DMF5, which is reactive to the MART-1 [27][28][29][30][31][32][33][34][35] epitope, was provided by John R. Wunderlich (Surgery Branch, NCI/National Institutes of Health). Protocols for generating mature monocyte-derived DCs over a 48-h period (rather than a 7-to 10-day period) have been described previously (73)(74)(75).…”

Section: Preparation Of Human Antigen-specific T Cells and Hla-a2mentioning

confidence: 99%

“…5a). DMF-5 cells carry TCR receptors specific for the MART-1 [27][28][29][30][31][32][33][34][35] sequence and respond to antigenspecific stimulation by producing IFN-␥ (83). Consistent with observed findings in the mouse system with the encapsulated OVA antigen, the MART-1 antigen encapsulated in NPs transpresenting multivalent IL-15:IL-15R␣ led to a greater IFN-␥ response from DMF5 cells compared with soluble IL-15:IL-15R␣ (Fig.…”

Section: Multivalent Il-15:il-15r␣ On Np Functions In the Samementioning

confidence: 99%

Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity

Hong

Usiskin

Bergamaschi

et al. 2016

Journal of Biological Chemistry

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It is well established that dendritic cells (DCs) 2 play a key role as professional antigen-presenting cells in initiating and coordinating antigen-specific adaptive immunity (1, 2). By constitutively sampling the external microenvironment through receptor-mediated and non-receptor mediated endocytic pathways, DCs initiate, program, and regulate the effector response against a variety of antigens (3-7). Although much is known about the immunobiology of DC antigen processing and presentation and the effect of different cytokines on DC maturation and their subsequent effect on the immune response (8), little is known about how the configuration or geometry of cytokine transmission affects the DC response. For example, physiologically, although IL-2 and IL-15 belong to the same four a-helix family of cytokines, IL-2 is transmitted as a soluble paracrine or autocrine factor (9, 10), whereas IL-15 is trans-presented on the surface of DCs and other cell types through a stable complex (11) with IL-15R␣ (12, 13) or secreted as a soluble paracrine complex (14, 15). The quality and manner by which cytokines are delivered to DCs can have a profound effect on the subsequent effector immune response (16,17).Nanosystems such as nanoparticles (18,19), nanotubes (20), or even macromolecular systems (21) comprise an emerging strategy for controlling antigen and cytokine transmission to . Perhaps one of the most explored carriers has been the polymeric poly(lactide-co-glycolide) (PLGA) system because of its significant promise and established effect in clinical settings (25-27) and well understood methods of formulation for delivery of small-molecule drugs, nucleic acids, and protein antigens (22, 28 -31). Several studies have demonstrated the promise of antigen-loaded PLGA nanoparticles as nanoparticle-based vaccines and delivery systems targeting DCs (32-39).IL-15 is a homeostatic cytokine for CD8 ϩ T cells and natural killer cells and an important regulator of the cytotoxic immune response (40). IL-15 signals by binding to cells expressing the IL-2/IL-15 ␤/␥ receptor and is found complexed to . This IL-15:IL-15R␣ complex is termed heterodimeric . IL-15R␣ is expressed by several cell types in * C. B. and G. N. P. are inventors on United States Government-owned patents and patent applications related to heterodimeric IL-15 and gene expression optimization. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

Cited by 406 publications

References 224 publications

A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line

A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity

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