In vitro pro-oxidant/antioxidant role of carvacrol, thymol and their mixture in the intestinal Caco-2 cell line

Llana-Ruiz-Cabello, María; Gutiérrez-Praena, Daniel; Puerto, María; Pichardo, Silvia; Jos, Ángeles; Cameán, Ana M.

doi:10.1016/j.tiv.2015.02.006

Cited by 123 publications

(80 citation statements)

References 33 publications

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“…This kind of slight effect in membrane permeabilization and proliferation can be anticipated when cancer cells, such as undifferentiated Caco-2 cells, are utilized as in vitro model. Thymol does not cause significant apoptosis or necrosis (Llana-Ruiz-Cabello et al, 2015), and this finding is supported by our study. Similarly, cinnamaldehyde is not cytotoxic at low amounts (Huang et al, 2011).…”

Section: Discussionsupporting

confidence: 90%

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Putaala¹,

Nurminen²,

Tiihonen³

2017

J. Anim. Feed Sci.

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Section: Discussionsupporting

confidence: 90%

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Putaala¹,

Nurminen²,

Tiihonen³

2017

J. Anim. Feed Sci.

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“…For instance, treatment of oxidized low density lipoprotein-activated THP-1 macrophages with OEO (10–30 μ g/mL) results in an overall reduction in proinflammatory cytokine release [24]. Likewise, antioxidative effects of OEO components thymol and carvacrol (8 to 30 μ g/mL) have been demonstrated in Caco-2 cells [25]. To our knowledge, this study is the first to demonstrate that OEO increases antioxidant enzyme expression levels and prevents oxidative stress-induced cell death through activation of Nrf2.…”

Section: Discussionmentioning

confidence: 99%

Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide‐Induced Oxidative Damage in IPEC‐J2 Cells

Zeng

Wang

Peng

et al. 2016

Oxidative Medicine and Cellular Longevity

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Oregano essential oil (OEO) has long been used to improve the health of animals, particularly their intestinal health. The health benefits of OEO are generally attributed to antioxidative actions, but the mechanisms remain unclear. Here, we investigate the antioxidative effects of OEO and their underlying molecular mechanisms in porcine small intestinal epithelial (IPEC-J2) cells. We found that OEO treatment prior to hydrogen peroxide (H2O2) exposure increased cell viability and prevented lactate dehydrogenase (LDH) release into the medium. H2O2-induced reactive oxygen species (ROS) and malondialdehyde (MDA) were remarkably suppressed by OEO. OEO dose-dependently increased mRNA and protein levels of the nuclear factor-erythroid 2-related factor-2 (Nrf2) target genes Cu/Zn-superoxide dismutase (SOD1) and g-glutamylcysteine ligase (GCLC, GLCM), as well as intracellular concentrations of SOD1 and glutathione. OEO also increased intranuclear expression of Nrf2 and the activity of an antioxidant response element reporter plasmid in IPEC-J2 cells. The OEO-induced expression of Nrf2-regulated genes and increased SOD1 and glutathione concentrations in IPEC-J2 cells were reduced by Nrf2 small interfering (si) RNAs, counteracting the protective effects of OEO against oxidative stress in IPEC-J2 cells. Our results suggest that OEO protects against H2O2-induced IPEC-J2 cell damage by inducing Nrf2 and related antioxidant enzymes.

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“…This phenolic compound plays an important role in the scavenging of free radicals such as DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid). In addition, at lower concentrations, it showed protective effects to cells against damage induced by the H 2 O 2 radicals 14,15 . Thus, the aim of this study was to assess the proliferation of human ADSCs in culture medium treated with natural antioxidants present in the L. origanoides Kunth essential oil.…”

mentioning

confidence: 99%

Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides

Brito

Vendramin²,

Lopes³

et al. 2018

Acta Cir. Bras.

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1 5-Experimental SurgeryActa Cir Bras. 2018;33(5):431-438 Abstract Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction . After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.

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In vitro pro-oxidant/antioxidant role of carvacrol, thymol and their mixture in the intestinal Caco-2 cell line

Cited by 123 publications

References 33 publications

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide‐Induced Oxidative Damage in IPEC‐J2 Cells

Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides

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