In vitro probiotic properties of Pediococcus pentosaceus L1 and its effects on enterotoxigenic Escherichia coli-induced inflammatory responses in porcine intestinal epithelial cells

Yin, Huajuan; Ye, Pengfei; Lei, Qingzhi; Cheng, Yandong; Yu, Hang; Du, J.; Pan, Hongbin; Cao, Zhenhui

doi:10.1016/j.micpath.2020.104163

Cited by 24 publications

(27 citation statements)

References 64 publications

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“…and Pediococcus sp. have been reported supporting our findings (Kang et al, 2011, Ahn et al, 2013, Lv et al, 2014, Mizoguchi et al, 2017, Yu et al, 2019, Bian et al, 2020, Yin et al, 2020.…”

Section: Discussionsupporting

confidence: 92%

Oral administration of Weissella and Pediococcus sp alleviates formalin induced inflammation in rats by Cytokine Modulation

Oladejo¹,

Oluwasola²

2021

ijs

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This study was designed to study the cytokine modulatory activity of three lactic acid bacteria (LAB) strains, Weisella cibaria II-1-59, Weisella confusa JMC 1093, and Pediococcus pentosaceus DSM20336 isolated from a Nigerian locally fermented food condiment; “iru” using paw oedema acute inflammatory model induced with 1% formalin in Wistar rats. Rats were distributed into six groups (A-F). Rats in Groups A were neither administered formalin nor treated with LAB, while Group B received formalin injection only. Rats in Groups C, D, and E were administered formalin and were treated orally with 2 × 107 CFU/ml of Weisella cibaria II-1-59, Weisella confusa JMC 1093, and Pediococcus pentosaceus DSM20336 respectively, while Group F received diclofenac sodium treatment following administration of formalin. The dose of LAB strain used for the oral treatment was 2 × 107 CFU/ml for all the groups while the dose of diclofenac sodium used was 150 mg/kg body weight of the rats. Paw thickness (mm) was checked at t = 0, 1, 4, 8, 24, 72, 168 and 336 h. Cytokine assay for C-reactive protein (CRP), Interleukin (IL-10) and Transforming growth factor (TGF-β) was performed on serum samples of the rats using Enzyme-linked immunosorbent assay (ELISA). Oral administration of W. cibaria II-1-59 showed the best significant decrease in the paw thickness of the rats, which was followed by P. pentosaceus DSM20336 and W. confusa JMC 1093 respectively, and was shown to be statistically significant at P<0.05. There was also a significant decrease (below standard 2000 pg/ml) in the secretion of pro-inflammatory biomarker (CRP) in all LAB treated groups at 1 hour, while there was an increase in the serum levels of anti-inflammatory cytokines IL-10 and TGF- β in Groups C-E rats which was maximally increased in W. confusa JMC 1093 treated rats. This study suggests that W. cibaria II-1-59, W. confusa JMC 1093 and P. pentosaceus DSM20336 possess anti-inflammatory potentials. Keywords: Inflammation, Cytokines, Weisella, Formalin, Oedema, Pediococcus

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Section: Discussionsupporting

confidence: 92%

Oral administration of Weissella and Pediococcus sp alleviates formalin induced inflammation in rats by Cytokine Modulation

Oladejo¹,

Oluwasola²

2021

ijs

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“…For example, the production of proinflammatory cytokines, such as TNF-α, is closely associated with the activation of NF-κB [101]. Probiotics have also been shown to exert their protective mechanisms against pathogenic infections by modulating the NF-κB signaling pathways [45,102,103]. In the current study, C. perfringens infection significantly elevated p38, JNK, and p65 phosphorylation in IPEC-J2 cells.…”

Section: Discussionsupporting

confidence: 47%

Protective Effects of Lactobacillus plantarum Lac16 on Clostridium perfringens Infection-Associated Injury in IPEC-J2 Cells

Zhou¹,

Wang²,

Wang³

et al. 2021

IJMS

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Clostridium perfringens (C. perfringens) causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of Lactobacillus plantarum (L. plantarum) Lac16 on C. perfringens infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that L. plantarum Lac16 significantly inhibited the growth of C. perfringens, which was accompanied by a decrease in pH levels. In addition, L. plantarum Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of C. perfringens to IPEC-J2 cells, and attenuated C. perfringens-induced cellular cytotoxicity and intestinal barrier damage. Furthermore, L. plantarum Lac16 significantly suppressed C. perfringens-induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, L. plantarum Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by C. perfringens infection. Collectively, probiotic L. plantarum Lac16 exerts protective effects against C. perfringens infection-associated injury in IPEC-J2 cells.

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“…LPS-stimulated RAW 264.7 macrophages were affected by GRC-ON89A-Hex, resulting in decreases in TNF-α, cyclooxygenase 2 (COX2) and inducible NO synthase (iNOS). Enterotoxigenic E. coli F4 + antagonism by P. pentosaceus L1 showed antiinflammatory effects by downregulating the levels of interleukin (IL)-6, tumour necrosis factor (TNF)-α and IL-8 [25]. Furthermore, oral administration of fermented C. militaris with P. pentosaceus ON89A in cyclophosphamide (CY)-induced mice improved its immunostimulatory abilities and obtain more nutrition acquisition [29,30].…”

Section: Probiotic Applicationsmentioning

confidence: 99%

Pediococcus pentosaceus, a future additive or probiotic candidate

et al. 2021

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Background Pediococcus pentosaceus, a promising strain of lactic acid bacteria (LAB), is gradually attracting attention, leading to a rapid increase in experimental research. Due to increased demand for practical applications of microbes, the functional and harmless P. pentosaceus might be a worthwhile LAB strain for both the food industry and biological applications. Results As an additive, P. pentosaceus improves the taste and nutrition of food, as well as the storage of animal products. Moreover, the antimicrobial abilities of Pediococcus strains are being highlighted. Evidence suggests that bacteriocins or bacteriocin-like substances (BLISs) produced by P. pentosaceus play effective antibacterial roles in the microbial ecosystem. In addition, various strains of P. pentosaceus have been highlighted for probiotic use due to their anti-inflammation, anticancer, antioxidant, detoxification, and lipid-lowering abilities. Conclusions Therefore, it is necessary to continue studying P. pentosaceus for further use. Thorough study of several P. pentosaceus strains should clarify the benefits and drawbacks in the future.

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In vitro probiotic properties of Pediococcus pentosaceus L1 and its effects on enterotoxigenic Escherichia coli-induced inflammatory responses in porcine intestinal epithelial cells

Cited by 24 publications

References 64 publications

Oral administration of Weissella and Pediococcus sp alleviates formalin induced inflammation in rats by Cytokine Modulation

Oral administration of Weissella and Pediococcus sp alleviates formalin induced inflammation in rats by Cytokine Modulation

Protective Effects of Lactobacillus plantarum Lac16 on Clostridium perfringens Infection-Associated Injury in IPEC-J2 Cells

Pediococcus pentosaceus, a future additive or probiotic candidate

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