In vitro production of embryos in swine

Abeydeera, Lalantha R.

doi:10.1016/s0093-691x(01)00670-7

Cited by 155 publications

(125 citation statements)

References 77 publications

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“…Germinal vesicle (GV) stage oocytes were collected for PDMH analysis or matured in vitro prior to IVF (Abeydeera, 2002). For PDMH analysis, COCs with numerous layers of intact cumulus cells were vortexed in 0.1% (w/v) hyaluronidase in Hepes-buffered saline for at least 5 min to remove the cumulus cells.…”

Section: Materials and Methods Oocyte Acquisition And In Vitro Maturamentioning

confidence: 99%

“…The zona free GV-stage oocytes were rinsed three times in DEPCtreated phosphate buffered saline (PBS) and flash frozen in liquid nitrogen prior to storage at À808C. For IVF the COCs were incubated in Tissue Culture Medium 199 (TCM199) (Gibco BRL, Grand Islands, NY) containing 0.14% (w/v) PVA, 10 ng/ml (w/v) epidermal growth factor (EGF), 0.57 mM (w/v) cysteine, 0.5 mg/ml (w/v) porcine follicle stimulating hormone and 0.5 mg/ml (w/v) porcine lutenizing hormone (Abeydeera, 2002). The maturation media was pre-equilibrated in 5% CO 2 in air at 398C in a humidified atmosphere overnight.…”

Section: Materials and Methods Oocyte Acquisition And In Vitro Maturamentioning

confidence: 99%

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Aberrant DNA methylation in porcine in vitro‐, parthenogenetic‐, and somatic cell nuclear transfer‐produced blastocysts

Bonk

Lai

et al. 2007

Molecular Reproduction Devel

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Early embryonic development in the pig requires DNA methylation remodeling of the maternal and paternal genomes. Aberrant remodeling, which can be exasperated by in vitro technologies, is detrimental to development and can result in physiological and anatomic abnormalities in the developing fetus and offspring. Here, we developed and validated a microarray based approach to characterize on a global scale the CpG methylation profiles of porcine gametes and blastocyst stage embryos. The relative methylation in the gamete and blastocyst samples showed that 18.5% (921/4,992) of the DNA clones were found to be significantly different (P < 0.01) in at least one of the samples. Furthermore, for the different blastocyst groups, the methylation profile of the in vitro-produced blastocysts was less similar to the in vivo-produced blastocysts as compared to the parthenogenetic-and somatic cell nuclear transfer (SCNT)-produced blastocysts. The microarray results were validated by using bisulfite sequencing for 12 of the genomic regions in liver, sperm, and in vivoproduced blastocysts. These results suggest that a generalized change in global methylation is not responsible for the low developmental potential of blastocysts produced by using in vitro techniques. Instead, the appropriate methylation of a relatively small number of genomic regions in the early embryo may enable early development to occur. Mol. Reprod.

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Section: Materials and Methods Oocyte Acquisition And In Vitro Maturamentioning

confidence: 99%

Section: Materials and Methods Oocyte Acquisition And In Vitro Maturamentioning

confidence: 99%

Aberrant DNA methylation in porcine in vitro‐, parthenogenetic‐, and somatic cell nuclear transfer‐produced blastocysts

Bonk

Lai

et al. 2007

Molecular Reproduction Devel

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“…OPU entails puncture of ovarian follicles. In sheep and goats, laparoscopy combined with OPU is a popular method (Pieterse et al, 1991;Carter et al, 2000;Galli et al, 2001;Abeydeera, 2002;Baldassarre et al, 2002;Cogine et al, 2003Cogine et al, , 2004Squires et al, 2003;Colleoni et al, 2007;Paramio, 2010).…”

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

“…The most frequently used methods of obtaining material from slaughterhouse samples are: puncture of an ovarian follicle with a needle connected to a suction pump (in all species), sectioning of ovaries (mostly in mares), and isolation of ovarian follicles and subsequent collection of oocytes (in all species) (Abeydeera, 2002;Cogine et al, 2003;Squires et al, 2003).…”

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

“…All of these requirements are characteristic of the respective farm animal species and have been described in detail in the literature: cattle (Hoshi, 2003;Lonergan, 2007;Lonergan and Fair, 2008), pigs (Abeydeera, 2002;Nagai et al, 2006;Lonergan and Fair, 2008;Gil et al, 2010;Dang-Nguyen et al, 2011), sheep and goats (Cognie et al, 2003;Paramio, 2010), and horses (Squires at el., 2003;Betteridge, 2006Betteridge, , 2007Hinrichs, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

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This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

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Oocyte Metabolism and Its Relationship to Developmental Competence

Krisher

Herrick

2013

Oocyte Physiology and Development in Domestic Animals

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In vitro production of embryos in swine

Cited by 155 publications

References 77 publications

Aberrant DNA methylation in porcine in vitro‐, parthenogenetic‐, and somatic cell nuclear transfer‐produced blastocysts

Aberrant DNA methylation in porcine in vitro‐, parthenogenetic‐, and somatic cell nuclear transfer‐produced blastocysts

Obtaining farm animal embryos <i>in vitro</i>

Oocyte Metabolism and Its Relationship to Developmental Competence

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