In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: A rare Himalayan medicinal orchid

Giri, Lalit; Dhyani, Praveen; Rawat, Sandeep; Bhatt, Indra D.; Nandi, Shyamal K.; Rawal, Ranbeer S.; Pande, Veena

doi:10.1016/j.indcrop.2012.01.024

Cited by 123 publications

(73 citation statements)

References 26 publications

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“…Collett. have been studied [13]. Suitability of hydroponic growing system as an alternative method of secondary metabolite production of Datura innoxia Mill [14] and Plumbago indica has been investigated [7].…”

Section: Optimization Of Suitable Sterilization Methods For Culture Esmentioning

confidence: 99%

Secondary Metabolites Contents and Antioxidant Capacities of Acmella Oleraceae Grown under Different Growing Systems

Abeysinghe¹,

Wijerathne²,

Dharmadasa³

2014

WJAR

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Acmella oleraceae (L.) R.K. Jansen (Asteraceae) is a therapeutically important medicinal plant used in traditional systems of medicine. Present study was undertaken to compare the total phenolic contents (TPC), total flavonoid contents (TFC), total antioxidant capacity (TAC) and Thin Layer Chromatography (TLC) profiles of areal parts and callus extracts of A. oleraceae grown under different growing systems (field grown, hydroponically grown and callus culture. Callus established in Murasige and Skoog (MS) medium, areal parts of field grown and hydroponically grown plants were extracted in 80% methanol. TPC, TFC and TAC were carried out by using colorimetric Folin-Ciocalteu method, aluminum nitrate method and Ferric Reducing Antioxidant Power (FRAP) assay respectively. TLC profiles were developed using established protocol. The best callus growth and the highest mean callus weight were observed in leaf explants established in MS medium supplemented with 2 mg/L BA and 1 mg/L IBA. Comparatively higher TPC (11.45 ± 0.17), TFC (12.33 ± 0.92) and TAC (10.27 ± 0.28) were observed in hydroponically grown plants. Order of TPC, TFC and TAC were increased as callus

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Section: Optimization Of Suitable Sterilization Methods For Culture Esmentioning

confidence: 99%

Secondary Metabolites Contents and Antioxidant Capacities of Acmella Oleraceae Grown under Different Growing Systems

Abeysinghe¹,

Wijerathne²,

Dharmadasa³

2014

WJAR

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“…3). Ease of synthesis causes its frequent accumulation in the biomass of in vitro plant cultures, for example, in Habenaria edgeworthii [30], Salvia officinalis [31], and Schisandra chinensis [32]. Its significant amount in C. ×incanus is associated with the production of catechins.…”

Section: In Vitromentioning

confidence: 99%

In vitro shoot cultures of pink rock-rose (Cistus ×incanus L.) as a potential source of phenolic compounds

Kubica

Szopa

2017

Acta Soc Bot Pol

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In vitro cultures of Cistus ×incanus (pink rock-rose) were maintained on two variants of Murashige and Skoog (MS) medium differing in terms of composition of plant growth regulators (PGRs): 6-benzyl adenine (BA) and 1-naphthaleneacetic acid (NAA) at the following concentrations: 3 mg/L and 0, 3 mg/L and 1 mg/L, respectively, and on a variant without PGRs -a control. Cultures were maintained in a form of agar and agitated shoot cultures. The qualitative and quantitative analyzes of three groups of phenolic compounds (catechins, flavonoids, and free phenolic acids) were performed by using the HPLC-DAD technique in methanolic extracts of in vitro biomasses and of commercial plant raw material. In analyzed extracts from in vitro cultures, the presence of catechin [max. 197.80 mg / 100 g dry weight (DW)], epicatechin gallate (max. 30.74 mg / 100 g DW), gallic acid (max. 83.23 mg / 100 g DW), quercetin (max. 10.15 mg / 100 g DW), and quercitrin (max. 72.89 mg / 100 g DW) was confirmed. The quantities of accumulated compounds varied and depended on the type of in vitro culture and the concentration of PGRs in media. The highest amounts of all estimated compounds were obtained in biomasses from agar cultures cultivated on medium without PGRs in vitro. In extracts obtained from commercial raw material, gallic acid (max. 261.80 mg / 100 g DW) and quercetin (max. 255.96 mg / 100 g DW) were detected as being the dominant compounds.

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“…The demand for natural antioxidants (plant phenolics) has increased tremendously over the past five years. New phenolic products are continuously been developed and consumed (Shao et al, 2008(Shao et al, , 2009BakowskaBarczak and Kolodziejczyk, 2011;Giri et al, 2012;Vadakkemuriyil Divya Nair et al, 2013). Our results have good significance, as this study provided economic and efficient ways to enhance phenolic content for commercial use.…”

Section: Introductionmentioning

confidence: 99%

Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves

Chen

Long

et al. 2013

Industrial Crops and Products

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In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: A rare Himalayan medicinal orchid

Cited by 123 publications

References 26 publications

Secondary Metabolites Contents and Antioxidant Capacities of <i>Acmella</i><i> </i><i>Oleracea</i><i>e</i> Grown under Different Growing Systems

Secondary Metabolites Contents and Antioxidant Capacities of <i>Acmella</i><i> </i><i>Oleracea</i><i>e</i> Grown under Different Growing Systems

In vitro shoot cultures of pink rock-rose (Cistus ×incanus L.) as a potential source of phenolic compounds

Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves

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