In Vitro Proliferation of Various Human Dental Stem Cells

Kook, Sun Young; Jo, You Young; Lee, Hee Jung; Seo, Byoung Moo; Choung, Pill Hoon

doi:10.4028/www.scientific.net/kem.342-343.165

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…It is believed that this tissue contains stem cells and lineage committed progenitor cells or precursor cells (PCs) for cementoblasts, periodontal ligament cells and osteoblasts. The results of study of Morsczeck et al [29,30], demonstrate that cultured PCs are unique undifferentiated lineage committed cells residing in the periodontium prior or during tooth eruption. Later on, they were designated as Dental Follicle Stem Cells (DFSC) and are extracted from dental follicle of the impacted teeth and possess multiple potentialities.…”

Section: Dental Follicle Stem Cells (Dfsc)mentioning

confidence: 99%

Stem Cells in the Oral Cavity

Silva¹

2015

Stud Stem Cells Res Ther

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Stem cells have emerged in the health area as a promise for the healing of damaged tissues and replacement of organs. With their capacity of self-renewal and clonogenity, as well as their ability to develop into many different cell types in the body, their potential seems to be unlimited, serving as a kind of cell stock for any cellular need in the human body. Nevertheless, their potential for odontology also seems to be promising for the simple possibility of tooth reconstruction and the reestablishment of shape and function. The aim of this review is to main sites in the oral cavity where these cells can be collected. implies, were collected from the dental pulp. Thereafter, four other kinds were cultivated namely: Stem Cells from Exfoliated Deciduous Teeth-SHED [4], Periodontal Ligament Stem Cells (PDLSCs), Stem Cells from Apical Papilla-SCAP [5] and Stem cells from third molars. These have been identified because of their ability to generate clonogenic adherent cell clusters when plated under the same growth conditions as described for Bone Marrow Stromal Cells (BMSSCs) [6,7]. Dental pulp stem cells (DPSC) DPSCs have three advantages over other stem cell sources widely researched. The first, and maybe more interesting advantage, is that they are possibly more prone to forming neurons than other stem cells, and neuron regeneration would be particularly interesting for Parkinson disease and spinal neuron regeneration. The second is that there are fewer ethical consideration than those which shroud other stem cells. Thirdly, they are more easily isolated than other stem cells, such as Mesenchymal Cells (MSCs). The first time Dental Pulp Stem Cells (DPSCs) were isolated was in 2000 in a research accomplished by Gronthos et al. [8], opening new possibilities for the use of these cells in many other tissues in the human body. Their source is the dental pulp mesenchyme (neural crest mesenchyme). The access for collection is not only easy, as it is also cheap while producing very low morbidity. The extraction of stem cells from the pulp can be considered as a highly efficient procedure and easily accomplishable, for the fact that there is a great tooth loss in patients under orthodontic therapy whose premolars are usually requested for removal. DPSCs are even slow cycling and represent mature adult pulp stem cells which, in vitro, are able to differentiate into odontoblasts, osteoblasts, adipocytes and endothelial cells, under appropriate growth factors. They are multipotent stromal cells that can be cryopreserved and used in several models of scaffolds, which can proliferate extensively, and build in vivo an adult bone with Havers channels and adequate vascularization, but with no proof of being able to produce dentin [9-12], while their easy management makes them feasible for clinical trials in human patients.. Subsequent studies [13-15], were made to understand the differentiative capacity of DPSCs, especially compared with Bone Marrow Mesenchymal Stem Cells (BMMSCs); the latter used as gold pattern in field of st...

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Section: Dental Follicle Stem Cells (Dfsc)mentioning

confidence: 99%

Stem Cells in the Oral Cavity

Silva¹

2015

Stud Stem Cells Res Ther

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Mechanisms Involved in Apice Closure of Pulpless Teeth – Literature Review

Neto¹,

Maia²,

Leão³

et al. 2021

TODENTJ

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Background: Unfinished root formation has always offered challenges in endodontics due to technical difficulties and weakened teeth resistance during the lifetime of an individual. Pulp revascularization therapy appeared as a solution for apical closure and root maturation. The existence of oral stem cells involved in the process associated with traditional resident cells requires adequate blood supply given by induced controlled injury deliberately accomplished into the periapical zone. Objective: The aim of this work was to research, through literature review, the main mechanisms involved in the process of apical closure through the technique of pulp revascularization. Conclusion: Apice closure in pulpless teeth seems to happen as a result of professional intervention and biological activity. The success rate depends on the role of traditional local immune cells and stem cells associated with adequate blood supply to finish root formation.

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In Vitro Proliferation of Various Human Dental Stem Cells

Cited by 2 publications

References 9 publications

Stem Cells in the Oral Cavity

Stem Cells in the Oral Cavity

Mechanisms Involved in Apice Closure of Pulpless Teeth – Literature Review

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