In Vitro Propagation and Regeneration of Several Hydrangea Genotypes

Sacco, E.; Savona, M.; Antonetti, Maurizio; Grassotti, A.; Pasqualetto, P.-L.; Ruffoni, B.

doi:10.17660/actahortic.2012.937.68

Cited by 5 publications

(5 citation statements)

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“…Feng Liu (2011) reported that, the highest frequency of leaf explants producing shoots (77%) and the highest mean number of shoots per explant (2.1) were observed on B5 medium supplemented with 2.25 mg/l BA and 0.1 mg/l IBA. Sacco et al, (2012) found that, in the multiplication phase the BA, at any concentration, induced the highest multiplication rate (over 7 shoots/explant) and the highest cluster fresh weight the shoot height was not affected by the cytokinin used in each treatment (data not shown). Very good quality explants were obtained using kinetin but the multiplication rate was not suitable for a commercial production.…”

Section: Effect Of Ba and Kin Concentration On Nodes Explant Of Hydramentioning

confidence: 89%

“…When rooted plantlets were transplanted in the greenhouse, they exhibited 100% survival rate. Sacco et al, (2012) reported that, the highest in vitro rooting percentage was achieved in the presence of NAA 0.5 mg L-1 (100% rooting).…”

Section: Rooting Stagementioning

confidence: 99%

“…Vigorous shoots of Hydrangea macrophylla were multiplied in vitro onto Murashige and Skoog (MS), 3% sucrose, supplemented with 0.25 mg/L of 6-benzyladenine (BA) and agarized with 8 g/L of technical Agar according to the protocol suggested by Sacco et al, (2012). In order to induce an efficient and functional in vitro root system, the shoots (2 cm) were cultured on full strength MS medium only and supplemented with indol acetic acid (IAA), indol butyric acid (IBA) or naphthalen acetic acid (NAA) at the same concentrations (0.5, 1.0, 2.0 and 4.0 mg/l) with or without activated charcoal (AC) 1g /l.…”

Section: Initial Source Of Explantmentioning

confidence: 99%

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Large Scales of Hydrangea macrophylla Using Tissue Culture Technique

Arafa¹,

Nower²,

Helme³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Effect Of Ba and Kin Concentration On Nodes Explant Of Hydramentioning

confidence: 89%

Section: Rooting Stagementioning

confidence: 99%

Section: Initial Source Of Explantmentioning

confidence: 99%

See 1 more Smart Citation

Large Scales of Hydrangea macrophylla Using Tissue Culture Technique

Arafa¹,

Nower²,

Helme³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Explants were cultured in MS nutrient medium containing sucrose (30 g/L). The multiplication of well-developed shoots was performed as described by Sacco et al [15]; rooting was induced, for instance, with IAA. The finally obtained plantlets were transferred into pots with an optimized soil mix (peat moss/sand, 3:1 v/v), for adaption in the greenhouse.…”

Section: Hydrangea Macrophyllamentioning

confidence: 99%

Culturing Important Plants for Sweet Secondary Products under Consideration of Environmentally Friendly Aspects

2022

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Some sweet tasting plant secondary metabolites are non-caloric or low nutritive compounds that have traditional use in food formulations. This mini-review focuses on conventional and advanced cultivation regimes of plants that accumulate sweet tasting or sweet taste modulating secondary metabolites of potential economic importance, in particular mogrosides (Siraitia grosvenorii), phyllodulcin (Hydrangea macrophylla), glycyrrhizin (Glycyrrhiza glabra), steviol glycosides (Stevia grosvenorii), and rubusoside (Rubus suavissimus). Consequential obstacles during the cultivation of Hydrangea macrophylla cultivars outside their natural habitat in a protected cultivation environment are addressed. Culturing at non-habitat locations facilitates short transportation routes of plant material for processing, which can be a key to an economically and environmentally compatible usage. The biosynthetic pathways, as far as known, are shortly mentioned. The proved or hypothetical degradation pathways of the compounds to minimalize environmental contamination are another focal point.

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“…Hydrangea macrophylla (hydrangea) is a deciduous shrub native to eastern Asia widely used as an ornamental plant in gardens and as cut flowers (Sacco et al, 2012). Hydrangeas are popular for their colorful, sphere-shaped inflorescence, which is highly demanded at Christmas and Mother's Day, mostly by the European and North American markets (van Gelderen and van Gelderen, 2004).…”

Section: Introductionmentioning

confidence: 99%

ROLE OF FLOWER PRESERVATIVE SOLUTIONS DURING POSTHARVEST OF Hydrangea macrophylla CV. BELA

et al. 2016

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Hydrangea macrophylla (hydrangea) is widely used as an ornamental plant and cut flower due to its inflorescence, which is composed of colored bracts. As cut flowers, hydrangeas show a postharvest life of up to 1 month at 2 °C, but little is known about their postharvest life during air shipment. This study was aimed at evaluating the effect of Triton X-100 (surfactant) and ClO 2 (biocide) during an air shipment simulation of hydrangea harvested at two floral stages of development: fresh and antique. Vase life, fresh weight loss (FWL) and solution uptake were evaluated after an air shipment simulation. Furthermore, bacterial counts and microscopic observation of the bracts were performed in order to understand the water relations of hydrangea during vase life. The longest vase life was observed in antique hydrangeas, and the control (deionized water) was the best treatment, reaching up to 32.7 days in this floral stage. FWL was faster when using Triton X-100 (3.83 days to lose 20% of FW) compared to deionized water (8.75 days). However, this flower preservative was efficient at promoting solution uptake (31.96 mL). Bacterial plugging did not appear to occur considering that the high presence of microorganisms counted did not affect solution uptake or vase life. The presence of stomata on the bracts seems to be crucial for the dehydration of the inflorescence, a problem that was not solved by the higher water uptake promoted by Triton X-100. Thus, deionized water was the best treatment, and the effect of flower preservatives in order to extend vase life of hydrangea cut flowers was insignificant.

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In Vitro Propagation and Regeneration of Several Hydrangea Genotypes

Cited by 5 publications

References 0 publications

Large Scales of Hydrangea macrophylla Using Tissue Culture Technique

Large Scales of Hydrangea macrophylla Using Tissue Culture Technique

Culturing Important Plants for Sweet Secondary Products under Consideration of Environmentally Friendly Aspects

ROLE OF FLOWER PRESERVATIVE SOLUTIONS DURING POSTHARVEST OF Hydrangea macrophylla CV. BELA

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