In Vitro Propagation and Synthetic Seeds Production: An Efficient Methods for Stevia rebaudiana Bertoni

Nower, Ahmed A.

doi:10.1007/s12355-013-0228-7

Cited by 35 publications

(17 citation statements)

References 22 publications

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“…Based on the completed study, it can be determined that addition of cytokinins to media has a stimulatory effect on the formation of the above-ground parts of stevia (Stevia rebaudiana Bertoni) plants-under their influence, the number of shoots and leaves has typically increased. An identical result was obtained by Nower (2014), where plants growing on media enriched with BAP at 0.5 and 1 mg•dm -3 concentration produced the highest number of shoots. Moreover, the study of ZAYOVA et al (2013) demonstrated that a medium with the addition of BAP at 1 mg•dm -3 concentration enables the production of a higher number of shoots in stevia than at 0.5 mg•dm -3 concentration.…”

Section: Propagation Stagesupporting

confidence: 78%

“…Considering its valuable properties, as well as highly impeded traditional stevia propagation methods (seeds demonstrate very low germination percentage, and the progeny plants exhibit high variability in terms of chemical composition and sweetness level), the need for development of an efficient method for mass clonal propagation of stevia in in vitro conditions appears to be valid (SOLIMAN, 2014, NOWER, 2014, KUMAR, 2013, DESHMUKH & ADE, 2012, ANBAZHAGAN et al 2010, PANICKER et al 2007, HWANG, 2006, SIVARAM & MUKUNDAN, 2003. However, in order for this to be possible, the parameters of the conducted culture must be precisely determined, and in particular the content of plant growth regulators in the medium.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Stevia rebaudiana plants

Rokosa

Kulpa

2020

Cienc. Rural

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The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm-3 IAA.

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Section: Propagation Stagesupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Micropropagation of Stevia rebaudiana plants

Rokosa

Kulpa

2020

Cienc. Rural

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“…This result indicates that the high level of Cytokinin alone gives best number of shoots as compared to combination with Auxin and absence of Cytokinin in medium decreased the length of shoot. Same result shows that high level of Cytokinin proved best for the shoot induction and shoot length as compared to combination with Auxin in this plant which is supported by 11 . He reported that the highest Numbers of Shoots produced on medium supplemented with BAP alone were best then other media.…”

Section: Results and Discussion:-supporting

confidence: 60%

In Vitro Shoot Multiplication of Stevia Rebaudiana (Bert) Through Plant Tissue Culture.

Bhingradiya¹,

Mankad²,

Patel³

et al. 2016

IJAR

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Stevioside obtained from the leaves of Stevia rebaudiana (Bert) is regarded as a valuable natural sweetening agent because of its relatively good taste and chemical stability. But Seed germination of the plant is poor. Due to low viability of seeds, present experiment attempts to study the effect of various basal salts and phytohormones on various aspects of shoot multiplication through in vitro technique. Various concentrations and combinations of BAP and NAA were used in MS medium and WPM medium and observed for the morphogenic responses of explants of Stevia rebaudiana (Bert). During the experiments it was observed that combination of WPM was the best performer in comparison with MS medium. It gave better growth in terms of number of shoots, length of shoots, number of internodes, multiplication rate and callus induction. …………………………………………………………………………………………………….... :-Stevia rebaudiana (Bert) is an herbaceous perennial plant belonging to Asteraceae family. It is a natural non caloric sweet tasting plant used around the world for its intense sweet taste. Diterpene glycosides produced by Stevia rebaudiana (Bert) leaves are many times sweeter than sucrose. They can be utilized as a substitute of sucrose 1,2,3,4,5,6.The potential uses of Stevia rebaudiana (Bert), is to produce stevioside, a non-caloric sweetener that does not metabolize in the human body. The sweet compounds pass through the digestive process without chemically breaking down, making Stevia safe for those who need to control their blood sugar level 7 . Stevia rebaudiana (Bert) is non-toxic, non-caloric, non-plaque, non-fermentative, flavor enhancing, non-carcinogenic, and has non-addictive sweetness. Stevioside and rebaudioside induce insulin secretion and the former acts as anti-tumor agent

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“…Badr-Elden (2013) showed that the storage medium supplemented with CCC, at all concentrations, the encapsulated buds of Achemia fasciata were still without regrowth after 2, 4 or 6 weeks. The growth of capsules of Stevia rebaudiana Bertoni dependent on mineral concentration and time of storage (Nower, 2014).…”

Section: Effect Of Aba Concentration In Gel Matrix and Conservation Pmentioning

confidence: 99%

New approaches for reducing the cost of the synthetic seeds storage using sugarcane bagasse and different additives to the gel matrix for sugarcane plant in vitro.

Badr-Elden

2018

Egypt. J. Bot.

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In Vitro Propagation and Synthetic Seeds Production: An Efficient Methods for Stevia rebaudiana Bertoni

Cited by 35 publications

References 22 publications

Micropropagation of Stevia rebaudiana plants

Micropropagation of Stevia rebaudiana plants

In Vitro Shoot Multiplication of Stevia Rebaudiana (Bert) Through Plant Tissue Culture.

New approaches for reducing the cost of the synthetic seeds storage using sugarcane bagasse and different additives to the gel matrix for sugarcane plant in vitro.

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