Stevioside obtained from the leaves of Stevia rebaudiana (Bert) is regarded as a valuable natural sweetening agent because of its relatively good taste and chemical stability. But Seed germination of the plant is poor. Due to low viability of seeds, present experiment attempts to study the effect of various basal salts and phytohormones on various aspects of shoot multiplication through in vitro technique. Various concentrations and combinations of BAP and NAA were used in MS medium and WPM medium and observed for the morphogenic responses of explants of Stevia rebaudiana (Bert). During the experiments it was observed that combination of WPM was the best performer in comparison with MS medium. It gave better growth in terms of number of shoots, length of shoots, number of internodes, multiplication rate and callus induction. …………………………………………………………………………………………………….... :-Stevia rebaudiana (Bert) is an herbaceous perennial plant belonging to Asteraceae family. It is a natural non caloric sweet tasting plant used around the world for its intense sweet taste. Diterpene glycosides produced by Stevia rebaudiana (Bert) leaves are many times sweeter than sucrose. They can be utilized as a substitute of sucrose 1,2,3,4,5,6.The potential uses of Stevia rebaudiana (Bert), is to produce stevioside, a non-caloric sweetener that does not metabolize in the human body. The sweet compounds pass through the digestive process without chemically breaking down, making Stevia safe for those who need to control their blood sugar level 7 . Stevia rebaudiana (Bert) is non-toxic, non-caloric, non-plaque, non-fermentative, flavor enhancing, non-carcinogenic, and has non-addictive sweetness. Stevioside and rebaudioside induce insulin secretion and the former acts as anti-tumor agent

Commiphoramukul found in India, Pakistan and Bangladesh. Guggul gum resin has been used to treat various diseases including cancer, atherosclerosis, rheumatism and obesity. The crude extracts obtained from the resin of Commiphoramukul in different solvents like ethyl acetate, methanol, acetone, and hexane were analyzed for phytochemical study. Secondary metabolites steroids, quinines, gum and mucilage, fixed oil and fats were found in all the four different extracts of Commiphoramukul however, oxalate and alkaloids were not found in any extract. Ethyl acetate, methanol, acetone, and hexane extracts from method-1(soxhlet) and method2 (cold) were tested for their ability to scavenge DPPH radical. Ethyl acetate extracts exhibited higher antioxidant activity (45.56%) compared to other solvent extracts. Various concentration of Commiphoramukul plant resin extract were used for MTT assay and highest % inhibition/Cytotoxicity (75.68 %) was found by methanol soxhlet extract having lowest IC 50 value (58.5µg/ml).