In vitro propagation of a useful tropical bamboo, Thyrsostachys siamensis Gamble, through shoot-derived callus

Obsuwan, K.; Duangmanee, Apisak; Thepsithar, C.

doi:10.1007/s13580-018-00119-z

Cited by 12 publications

(5 citation statements)

References 25 publications

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“…The combination 18 µM 2,4-D + 9 µM 2iP was xed as the ideal for callus induction due to the high induction effect associated with a low oxidation rate in the in orescence explants. The 2,4-D has been reported for callogenesis and somatic embryogenesis in several bamboo species (Obsuwan et al, 2019). In young shoots of D. hamiltonii (Godbole et al, 2002) and zygotic embryos of P. edulis (Yuan et al, 2013), callus induction occurred only in a culture medium containing 2,4-D, and the induction frequency was affected by its concentration.…”

Section: Callus Induction and Oxidationmentioning

confidence: 98%

Somatic embryogenesis from young inflorescences of the giant bamboo Dendrocalamus asper (Schult f.) Backer ex Heyne

Ornellas

Fritsche

Medina

et al. 2021

Preprint

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Bamboos are an important worldwide non-timber forest product with current rising interest due to their environmentally friendly applications. Besides the consolidated uses of the sweet shoots and culms for structural uses, Dendrocalamus asper is an imposing ornamental bamboo for horticulture. The present work aimed to establish in vitro calli culture and plant regeneration through somatic embryogenesis starting from young inflorescences of the giant bamboo, D. asper. Pre-anthesis inflorescences were collected, disinfested, and subjected to callus induction on MS basal medium supplemented by 0 µM, 9 µM, 18 µM, 27 µM, and 36 µM of 2,4-D in combination with 9 µM of 2-iP or 9 µM Kin. The different obtained calli types were characterized and subcultured in 0 µM, 4.5 µM, 9 µM, and 18 µM of 2,4-D in combination with 9 µM of both cytokinins for multiplication and differentiation. Additionally, the explant incision and its inoculation orientation onto culture media were tested for callus induction improvement. The 2,4-D was essential for callus induction, and its combination with both cytokinins resulted in embryogenic callus induction and further somatic embryos regeneration. The subsequent reduction of this auxin to 4.5 µM resulted in somatic embryo maturation. Somatic embryos transferred to a plant growth regulator-free medium resulted in plantlet conversion. The present work showed the feasibility of using inflorescences as explants and the efficiency of using the 2-iP in combination with 2,4-D to callus induction and in vitro bamboo plant regeneration through somatic embryogenesis.

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Section: Callus Induction and Oxidationmentioning

confidence: 98%

Somatic embryogenesis from young inflorescences of the giant bamboo Dendrocalamus asper (Schult f.) Backer ex Heyne

Ornellas

Fritsche

Medina

et al. 2021

Preprint

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“…For the preparation of media, Murashige and Skoog (1962) medium (MS medium) including vitamins (1 fold MS medium 4.4 g•L -1 and ½ fold MS medium 2.2 g•L -1 ) with/without growth regulators (kinetin and BA), sucrose (20.0 g•L -1 ), coconut water (20.0 ml•L -1 ), and Gelrite (2.0 g•L -1 ) was used (Table 1). In a previous study, the leaf explants of endangered Haworthia were cultured using kinetin/BA with NAA (Rogers, 1993;Giusti et al, 2002;Obsuwan et al, 2019). Instead of coconut milk, coconut water was used in all medium compositions both in 1 fold MS and in ½ fold MS media with/without growth regulators (Kaul and Sabharwal, 1972).…”

Section: Culture Media and Culture Conditionsmentioning

confidence: 99%

Role of Growth Regulators in the Somatic Organogenesis of Haworthia Inflorescences in Vitro

Yesmin¹,

Mazharul²,

Kim³

et al. 2020

HST

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This study investigated the effect of growth regulators on the somatic organogenesis of Haworthia inflorescences. The inflorescences of five Haworthia cultivars and one species were cultured on 1X and 0.5X MS media with/without growth regulators for organogenesis. Shoot and callus induction was observed for three of the five cultivars, whereas only callus induction was observed for the other two cultivars and one species. Of the two growth regulator-free media used, all explants performed better on ½ fold MS medium than on 1 fold MS medium in terms of shoot and callus induction. Conversely, among the six media supplemented with kinetin and 6-benzylaminopurine (BA), there was no shoot formation; however, a different response in callus formation was observed for H. splendens and 'White Wolf'. The callus induction of H. splendens was more vigorous than that of 'White Wolf'. In this study, better organogenesis from young upper parts of the inflorescences was observed. The highest shoot and root multiplication was observed on NAA containing medium, and no root formation was observed on BA containing medium. The highest shoot multiplication (20.8 ± 0.29) was observed for 'Tiffany × Fertenon B Com' on 1.4 mg•L -1 BA containing medium within 18 weeks, whereas, the highest root induction was observed for Haworthia 'Natalie' on medium containing 0.05 mg•L -1 NAA. The results revealed that different parts of Haworthia inflorescences showed different organogenesis responses. Therefore, this study contributes to a better understanding of the organogenesis response of Haworthia cultivars on different media.

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“…Micropropagation of bamboos is accepted as one of the standard methods for the large-scale multiplication of bamboo clones with desirable characteristics. This is achieved by germination of seeds in vitro (Lv et al, 2021) to overcome the low germination rate of bamboo seeds using conventional approaches, by the in vitro establishment of axillary shoots (Sandhu et al, 2018), the induction of callus tissue (Obsuwan et al, 2019;Zang et al, 2016) and organogenesis (Dey et al, 2020). Methods developed for the micropropagation of bamboo must be optimized based on the variety and the type of the tissue (Ara et al, 2020) and this is achieved by the fine tuning of the components of the culture medium as well as the selection of the appropriate concentrations of the carbon sources (Yasodha et al, 2008), the concentration and the ratio of the phytohormones (Venkatachalam et al, 2015), and the mitigation of the activity of polyphenol oxidases in vitro that can contribute to abscission of growing tissue (Huang et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Establishment of An in Vitro Mass Propagation System for Dendrocalamus asper.

Mustafa,

Lym Yong,

Kulip

et al. 2023

JTBC

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Dendrocalamus asper is a species of bamboo that has high commercial value and is the bamboo of choice for large scale agro-forestry plantations in the tropical regions of the world. Micropropagation using tissue culture is essential to generate uniform clones that are amenable to establishment in industrial agro-forestry projects for bamboo biomass, habitat restoration or in carbon sequestration. This paper reports on the establishment of D. asper invitro using commercially available seeds. The seeds were surface sterilized using three different chemical agents which were Sodium Hypochlorite (20%), Mercuric Chloride (0.1%) and Ethanol (70%) followed by shoot initiation on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) with a concentration ranging from 1.0 - 5.0 mg/L. Propagules were multiplied on MS media supplemented with different concentration of IBA Indole-3-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA), and finally rooted and hardened in peat moss. The findings of our study indicate that the sterilization protocol eliminated all the plant pathogens, resulting in an axenic culture. Full strength MS medium supplemented with 5 mg/L BAP yielded the highest number of shoots (11.46 per explant) after four weeks of inoculation. The highest multiplication rate (3.95 shoots per explant) was obtained on MS medium supplemented with 3 mg/L BAP. The time required from initiation to hardening was 70 to 90 days, following which the plantlets were ready for field trials. The findings of this study will facilitate the establishment of plant tissue culture programmes dedicated to the production of D. asper locally, thus eliminating the need for imports and the possible entry of plant pathogens that can be detrimental to the local agro-forestry industry.

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In vitro propagation of a useful tropical bamboo, Thyrsostachys siamensis Gamble, through shoot-derived callus

Cited by 12 publications

References 25 publications

Somatic embryogenesis from young inflorescences of the giant bamboo Dendrocalamus asper (Schult f.) Backer ex Heyne

Somatic embryogenesis from young inflorescences of the giant bamboo Dendrocalamus asper (Schult f.) Backer ex Heyne

Role of Growth Regulators in the Somatic Organogenesis of Haworthia Inflorescences in Vitro

Establishment of An in Vitro Mass Propagation System for Dendrocalamus asper.

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