In Vitro Propagation of Agave americana by Indirect Organogenesis

Lecona-Guzmán, Carlos Alberto; Reyes-Zambrano, Sheila Jazmín; Barredo-Pool, Felipe; Abud‐Archila, Miguel; Montes-Molina, Joaquín A.; Rincón-Rosales, Reiner; Gutiérrez-Miceli, Federico Antonio

doi:10.21273/hortsci10498-16

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…The highest number of shoots of Aloe vera was obtained in medium containing BAP comparison with another cytokinins (Lavakumaran and Seran, 2014). Agave Americana planted on MS medium supplemented with BAP had the highest number of shoots per callus after 4 weeks from culturing (Lecona-Guzmán et al, 2017). Shoots regenerated of Agave angustifolia from shoot tip on the media with 0.4 mg/l produced the best number of shoots.…”

Section: Introductionmentioning

confidence: 92%

Effect of Media Type and BAP Concentrations on Micropropagation during Multiplication Stage on Ponytail Palm (Beaucarnea recurvata Lem.)

Kawy¹

2018

HJSC

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Ponytail palm (Beaucarnea recurvet Lem.; Family Asparagaceae) is one of the most important plants in the internal and external coordination. This work was carried out to study the effect of media type (MS, B5 and WPM) and Benzylaminopurine BAP at 0, 0.2, 0.4 and 0.6 mg/l during multiplication stage. The shoot tips were collected from in vitro seedlings cultured on MS medium without growth regulators. B5 medium supplemented with 0.4 mg/l (BAP) increased number of shoots (3.40 shoots/clump) and number of leaves (24) plant compared with other treatments. The B5 medium is preferable within mass production and featured commercial. The BAP Concentration 0.4 mg/l promotes shoots initiation and development with B5 medium more than MS and WPM.

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Section: Introductionmentioning

confidence: 92%

Effect of Media Type and BAP Concentrations on Micropropagation during Multiplication Stage on Ponytail Palm (Beaucarnea recurvata Lem.)

Kawy¹

2018

HJSC

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“…For callus growth in a liquid medium, two strategies were used. The first strategy consisted of growing the selected callus in the same callus induction treatment, and the second strategy consisted of using combinations of hormones: the first treatment was using the combination of 2,4-D + 6-Benzylaminopurine (BAP) (0.5 + 9 mg L −1 ) [16], and the second treatment was using the combination of naphthaleneacetic acid (NAA) + BAP (0.5 + 0.5 mg L −1 ) [17]. Cell viability was determined using the Trypan blue technique [18].…”

Section: Induction Of Cell Suspension Of Agave Americana Lmentioning

confidence: 99%

Effect of Water Stress Induced by Polyethylene Glycol on Growth, Proline Accumulation in Agave americana L.

Enrique¹,

Alberto²,

Antonio³

et al. 2023

Phyton

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The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L −1 ) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established using 0.5 mg L −1 naphthaleneacetic acid (NAA) + 0.5 mg L −1 Benzylaminopurine (BAP). Seedlings were exposed to polyethyleneglycol (15% and 30% w/v) with a water potential of −0.87 and −2.67 MPa, respectively, under soil conditions. Water stress was applied through restricted irrigation. Fresh weight, root system growth, and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on A. americana L. Chlorophyll concentration values were significantly decreased by 15 at 30% PEG (19.6 SPAD units) compared to the control treatment. In in vitro plants, the highest concentration of proline was found in the roots, being the treatment with 30% polyethylene glycol where the highest concentration of this osmoregulator was obtained (62.5 mg g −1 DW). Under restricted irrigation conditions, an increase in proline concentration was observed both in the aerial part (2.2 µg 100 g −1 DW) and in the root system (1.8 µg 100 g −1 DW). However, the concentrations found were approximately ten times greater, less than those found under in vitro conditions. Therefore, the accumulation of proline can be considered an indicator of stress in Agave Americana L. growth in vitro.

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