In vitro propagation of an endangered plant species, Thermopsis turcica (Fabaceae)

Cenkci, Süleyman; Kargıoğlu, Mustafa; Dayan, Sergun; Konuk, Muhsin

doi:10.2478/s11756-008-0125-9

Cited by 17 publications

(12 citation statements)

References 18 publications

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“…This finding is also supported by Zhao et al (2010) and Tohidi-Moghadam et al (2009). Whereas for NAA, low callusing index was also reported by Cenkci et al (2008). This finding was contradicted, who reported that the hypocotyls explant of Lycopersicon esculentum cultured on MS medium supplemented with 0.5 mg L −1 NAA gave the highest callusing index.…”

Section: Discussioncontrasting

confidence: 49%

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Tan¹,

Musa²,

Ariff³

et al. 2010

American Journal of Biochemistry and Biotechnology

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Problem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog (MS) medium containing B5 vitamins and 30 g L −1 sucrose supplemented with different concentrations (0.5-2.5 mg L ). This combination was also found to be best for callus proliferation for all the accessions investigated. Among the four accessions tested, UPM03 was found to have the highest biomass yield (0.041 g DW culture

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Section: Discussioncontrasting

confidence: 49%

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Tan¹,

Musa²,

Ariff³

et al. 2010

American Journal of Biochemistry and Biotechnology

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“…To date the effect of zeatin has been reported by different research groups (Dirks & Buggenum 1989;Kováč 1992;Malik & Saxena 1992;Gaba et al 1999;Nasircilar et al 2011); similar results were also described in these studies. The shoot regeneration of T. turcica on the MS medium containing different concentrations of NAA was also reported by Cenkci et al (2008). The differences between the present study and that of Cenkci et al (2008; were as follows: the concentrations and combinations of growth regulators used in the media, the source of the explant used for the research, and the selected population of T. turcica.…”

Section: Plant Regenerationcontrasting

confidence: 45%

In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Tekdal

Çetiner

2014

Biologia

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This plant tissue study of micropropagation identifies the selective medium saving for rapid propagation in cultivated Thermopsis turcica, an endangered germplasm of the family Fabaceae. The aim is to obtain the optimum growth medium of T. turcica by enabling the in vitro propagation of this endemic. In this study, the leaves and stems of T. turcica were cultured on a Murashige and Skoog's medium supplemented with various concentrations (0.5, 1.0 and 2.0 mg L −1 1-Naphthaleneacetic acid) of auxin and (0.2 and 0.5 mg L −1 Zeatin) (1.0 and 2.0 mg L −1 Benzylaminopurine) of cytokinins. Previous research focused on the regeneration from the seed of T. turcica Eber population; we concentrated upon the regeneration of different plant parts (leaf and stem) of T. turcica Aksehir population. In addition, according to the literature on T. turcica that to date the effects of Zeatin on the regeneration has not been performed. The most promising regeneration and growth were obtained from leaf explants cultured on the media with 2.0 mg L −1 1-Naphthaleneacetic acid and 0.5 mg L −1 Zeatin (93.3%). The regenerated plantles were rooted on the media containing 2.0 mg L −1 Indole-3-butyric acid. Rooted plantlets were transplanted into potting of sterilized soil. The present study reports on the sufficient in vitro regeneration protocol through organogenesis in T. turcica. The findings presented here have implications for in vitro protection and use of this endemic endangered species in further biotechnological research.

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“…An in vitro propagation system provides plants with uniform genetic identity and can ensure clean planting materials (Shatnawi et al, 2011). It provides complementary conservation options for plant species with limited reproductive capacities including a number of rare and endangered species (Cenkci et al, 2007). Reports have been published on in vitro propagation of Curcuma zedoaria and C. longa using various explants such as rhizome sprout (Loc et al, 2005), bud or shoot-tip (Salvi et al, 2002;, leaf-base (Salvi et al, 2001), and immature inflorescence (Salvi et al, 2000).…”

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confidence: 99%

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Sharmin¹,

Alam²,

Sheikh³

et al. 2013

Turk J Biol

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IntroductionCurcuma aromatica Salisb. (Zingiberaceae), indigenous to South Asia, is a robust zingiber with stout underground rhizomes. This wild, aromatic, and attractive turmeric is probably the most useful among the turmeric members for its unique medicinal values. C. aromatica rhizome is a rich source of volatile oil, which consists of several major anti-tumor ingredients including demethoxycurcumin, β-elemene, curcumol, curdione, etc. (Zhou et al., 1997;Dulak, 2005). C. aromatica could promote blood circulation to remove blood stasis and treat cancers and angiogenesis (Kim et al., 2002). The oil infused via the hepatic artery has proven to exert ideal therapeutic effects in humans with primary liver cancer and rats with transplanted hepatoma (Cheng et al., 1999). Curcumin, the most prevalent active secondary ingredient in C. aromatica, acts as a promising agent in the treatment and/or prevention of Alzheimer's disease (Ringman et al., 2005). In addition, the rhizome is Abstract: A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. When cytokinins, such as benzyl amino purine (BAP) or 6-(α,αdimethylallylamino)-purine (2iP), were used either singly or in combination with naphthalene acetic acid (NAA) for shoot induction and multiplication, a single use of BAP was the most effective. As a carbon source, 3% (w/v) sucrose exhibits the greatest promotive effect on shoot initiation and proliferation compared with other carbon sources used. Among the basal media, full strength Murashige and Skoog (MS) media produced the best results, compared to other media studied. By using the most effective treatment from each category, an average of 13.2 shoots/per explant were produced after 6 weeks of culture. Moreover, 85% survival was achieved when rooted explants acclimatized ex vitro using a mixture of sterile sand, soil, and farmyard manure (1:1:1). In addition, antimicrobial activities of rhizome extracts were evaluated. Petroleum ether and chloroform extracts of field-grown rhizome showed potential antimicrobial properties against several human pathogenic bacteria including Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella sonnei, and Shigella dysenteriae, with a minimum inhibitory concentration (MIC) ranging from 0.03 to 0.5 mg/mL. Thus, the optimized micropropagation protocol may offer large-scale production of plantlets to meet industrial demand for the rhizome. Moreover, our results suggest the rhizome extract of C. aromatica is a promising antimicrobial agent.

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In vitro propagation of an endangered plant species, Thermopsis turcica (Fabaceae)

Cited by 17 publications

References 18 publications

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

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