In vitro propagation of Anathallis adenochila (Loefgr.) F. Barros (Orchidaceae), a species endemic to southern and southeastern Brazil

Júnior, Delio Endres; Sasamori, Márcio Hisayuki; Droste, Annette

doi:10.1590/0102-33062014abb3158

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…Other than that, to further support the results as seen in Figure 1 , in the same study, the inability of maltose to produce PLBs was also reported in the study Sanjaya et al (2007). On top of that, the same sucrose concentration which is 30 g/L was also found out to be optimal for protocorms development of orchids were reported by Júnior et al (2014) which further verify the results obtained from this study.…”

Section: Resultssupporting

confidence: 88%

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Rahman

Othman

Amran

et al. 2021

Int. J. Res. Granthaalayah

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Micropropagation of orchids are often through the production of Protocorm Like Bodies (PLBs) and this research conducted a study to discover the optimal condition for the growth of Protocorm Like Bodies (PLBs) and also plantlets regeneration of Grammatophyllum speciosum Several aspects such as different carbon sources (sucrose, maltose) and the presence of charcoal alongside with the variety of concentration of agar were taken into account for the optimization. This study discovered that the 30 g/L of sucrose in 1 mg/L of BAP was superior in producing heavy PLBs. However, the trend differs in the number of plantlets where maltose (5 g/L) is seen to produce a higher number of plantlets compared to sucrose. Charcoal plays an important role in the growth of PLBs where the heaviest PLBs were recorded in the medium supplemented with 0.20 % of charcoal along with 4.5 g of agar. Vice versa relationship was recorded for the number of plantlets where without charcoal in 4.5 g of agar produce the highest number of plantlets.

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Section: Resultssupporting

confidence: 88%

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Rahman

Othman

Amran

et al. 2021

Int. J. Res. Granthaalayah

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“…For example, Anathallis adenochila (Loefgr.) F. Barros was found to take 12 months to develop seedlings (> 0.5 cm in height) [ 21 ]. In another species of the Pleurothallidinae subtribe, Restrepia brachypus Rchb.f., germination ranged from 7.96% in MS medium to 53.05% in Western (W) medium [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most species of Pleurothallidinae have no great commercial appeal, due to the small dimensions of the plants and their flowers, as well as the difficulties of cultivation [ 18 ]. There are few studies of asymbiotic germination of micro-orchids, which have shown low germination rates and slow growth of seedlings [ 21 , 22 ]. The best results were achieved by Koene et al [ 13 ] with 79% of total germination of A .…”

Section: Introductionmentioning

confidence: 99%

Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

et al. 2020

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The morphological and morphometric characters of seeds belonging to 11 species of the subtribe Pleurothallidinae using light and scanning electron microscopy were studied to understand the in vitro germination process. Qualitative data (color, shape, ornamentation) and quantitative ones were also evaluated in seeds and embryos (length, width, volume and air space percentage between the integument and the embryo). The viability of the seeds was evaluated by in vitro germination in woody plant medium (WPM), and by analysis of the developmental stages of protocorms until seedling formation (two to 24 weeks). Morphometric data showed variations within the genus Acianthera and between species of different genera. The best germination and protocorm formation responses occurred with Acianthera prolifera (92%) and Acianthera ochreata (86%), with the formation of seedlings after 12 and 16 weeks of sowing, respectively. The seeds and embryos of A. prolifera and A. ochreata were larger (length, width, and volume) with a structural polarity that may have facilitated their germination comparing to others studied species. Other characteristics of A. prolifera seeds that may have contributed to these results include the presence of a thin testa without ornamentation and a suspensor. The protocorms of Anathalis obovata, Dryadella liliputiana, and Octomeria gracillis developed slowly in the WPM, not reaching the seedling stage in 24 weeks of cultivation. This morphological and morphometric study contributes to the understanding of asymbiotic germination of some micro-orchid species.

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“…It could be noted that Brazil has the highest number of published works both on propagation and germplasm conservation, while for some countries only researches referring to propagation of native orchid species are reported and for other countries (Guyana, Paraguay, Trinidad and Tobago, Suriname, Uruguay) there were no registered works in any of the topics. Almeida et al 2017;Caovila et al 2016;Cardoso and Ono 2011;Cavalcante Martini et al 2001;Corrêa Pereira et al 2011;2015;Da Silva et al 2014;Da Silva Júnior et al 2012;De Carvalho et al 2018;De Conti et al 2018;De Melo Ferreira et al 2017;De Menezes Gonçalves et al 2012;Dezan et al 2012;Dignart et al 2009;Dorneles and Trevelin 2011;Endres Júnior et al 2014;Favetta et al 2017;Galdiano Júnior et al 2011;2012a,b;Gomes de Araújo et al 2009;Hosomi et al 2017;Lando et al 2016;Lone et al 2010;Mantovani et al 2016;Massaro et al 2012;Monteiro do Rêgo et al 2009;Moraes et al 2005;Pasqual et al 2011;Pedroso-de-Moraes et al 2012;Pereira et al 2018;Pereira Gomes et al 2015;Prizão et al 2012;Rodrigues et al 2015;Rodrigues Guimarães et al 2013;Sasamori et al 2015;…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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In vitro propagation of Anathallis adenochila (Loefgr.) F. Barros (Orchidaceae), a species endemic to southern and southeastern Brazil

Cited by 9 publications

References 20 publications

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

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