In Vitro Propagation of Pistacia Species

Barghchi, M.; Alderson, P. G.

doi:10.17660/actahortic.1983.131.4

Cited by 32 publications

(16 citation statements)

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“…The initiation of cultures from mature trees has been reported by a few researchers (Barghchi and Martinelli 1984;Gonzales and Frutos 1990;Onay 2000b, Tilkat 2006Tilkat et al 2008). There have also been several previous reports on plant regeneration from seedling explants of other pistachio species: wild rather than cultivated species (Barghchi and Alderson 1983b;Barghchi 1986b, Onay 2000cTilkat 2003;Tilkat et al 2005). Pistachio regeneration has also been investigated by in vitro micrografting (Abousalim and Mantell 1992;Onay et al 2004).…”

Section: Introductionmentioning

confidence: 90%

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Direct shoot organogenesis from in vitro-derived mature leaf explants of pistachio

Tilkat

Onay

2008

In Vitro Cell.Dev.Biol.-Plant

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An efficient system was developed for direct plant regeneration from in vitro-derived leaf explants of Pistacia vera L. cv. Siirt. The in vitro procedure involved four steps that included (1) induction of shoot initials from the regenerated mature leaf tissue, (2) regeneration and elongation of shoots from the shoot initials, (3) rooting of the shoots, and (4) acclimatization of the plantlets. The induction of shoot initials was achieved on an agarified Murashige and Skoog (MS) medium with Gamborg vitamins supplemented in different concentrations of benzylaminopurine (BA) and indole-3-acetic acid (IAA). The best medium for shoot induction was a MS medium with 1 mgl −1 IAA and 2 mgl −1 BA. Numerous shoot primordia developed within 2-3 wk on the leaf margin and the midrib region, without any callus phase. In the second step, the shoot clumps were separated from the leaf explants and transferred to a MS medium supplemented with 1 mgl −1 BA, resulting in a differentiation of the shoot initials into well-developed shoots. The elongated shoots (>3 cm long) were rooted on a full-strength MS basal medium supplemented with 2 mgl −1 of indole-3-butyric acid in the third stage. Finally, the rooted plants were transferred to soil with an 80% success rate. This protocol was utilized for the in vitro clonal propagation of this important recalcitrant plant species.

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Section: Introductionmentioning

confidence: 90%

“…Tissue culture of the species began in the early 1980s (Barghchi 1982). Regeneration from P. vera L. with seed or seedling tissues has been the most successful to date (Barghchi and Alderson 1983a, b;1985;Yang and Ludders 1993;Parfitt and Almehdi 1994;Dolcet Sanjuan and Claveria 1995;Onay et al 1995Onay et al , 2000Onay 2000a).…”

Section: Introductionmentioning

confidence: 99%

Direct shoot organogenesis from in vitro-derived mature leaf explants of pistachio

Tilkat

Onay

2008

In Vitro Cell.Dev.Biol.-Plant

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“…Most authors recommend the use of MS as a basal culture medium for the micropropagation for different species of Pistacia as P. vera, P. khinjuk, P. lenthiskus, P. atlantica (Barghchi and Alderson, 1983a,b, 1985; Yang and Lüdders, 1993; Benmahioul et al, 2012; Akdemir et al, 2014). Other media specifically design for woody plants, as DKW (Driver Kuniyuki Walnut medium; Driver and Kuniyuki, 1984) or WPM (Woody Plant Medium; Lloyd and McCown, 1980) for P. vera L. (Gonzalez and Frutos, 1990; Benmahioul et al, 2009) have also been applied.…”

Section: Introductionmentioning

confidence: 99%

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

et al. 2018

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The aim of this study was to determine the effects of Murashige and Skoog (MS) salts on optimal growth of two pistachio rootstocks, P. vera cv. “Ghazvini” and “UCB1” using design of experiments (DOE) and artificial intelligence (AI) tools. MS medium with 14 macro—and micro-elements was used as base point and its concentration varied from 0 to 5 × MS concentrations. Design of experiments (DOE) software was used to generate a five-dimensional design space by categorizing MS salts into five independent factors (NH4NO3, KNO3, mesos, micros and iron), reducing the experimental design space from 3,125 to just 29 treatments. Typical plant growth parameters such as shoot quality (SQ), proliferation rate (PR), shoot length (SL), and some physiological disorders including shoot-tip necrosis (STN) and callus formation at the base of explants (BC) were evaluated for each treatment. The results were successfully modeled using neurofuzzy logic software. The model delivered new insights, by different sets of “IF–THEN” rules, pinpointing the key role of some ion interactions (SO42- × Cl−, K+ × SO42- × EDTA−, and Fe2+ × Cu2+ × NO3-) for SQ, PR, and SL, whilst physiological disorders (STN and BC) were governed mainly by independent ions as Fe2+ and EDTA−, respectively. In our opinion, the methodology and results obtained in this study is extremely useful to understand the effect of mineral nutrients on pistachio in vitro culture, through discovering new complex interactions among macro—and micro-elements which can be implemented to design new media of plant tissue culture and improve healthy plant micropropagation for any plant species.

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“…Seed and seedling tissues, however, have produced promising results in pistachio (Barghchi & Alderson 1983;Martinelli 1988;Picchioni & Davies 1989) and mango (Litz et al 1982;Dewald et al 1989;Jaiswal 1990). Seed and seedling tissues, however, have produced promising results in pistachio (Barghchi & Alderson 1983;Martinelli 1988;Picchioni & Davies 1989) and mango (Litz et al 1982;Dewald et al 1989;Jaiswal 1990).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Anacardium occidentale L.

D’Silva¹,

D’Souza²

1992

Plant Cell Tiss Organ Cult

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Multiple buds have been induced from cotyledonary nodes of cashew, Anacardium occidentale L.Maximum number of buds were initiated on Murashige & Skoog's (MS) medium supplemented with 117 mM sucrose, 14.6 mM maltose and 22.2 IxM BA. The buds were harvested at each subculture after elongation on MS medium supplemented with 100mll -~ coconut water, 14.6mM maltose, increased sucrose (146mM) and decreased BA (4.4p~M). Excised microshoots were rooted in vitro on MS medium in the presence of 117 mM sucrose, 2.9 txM IAA and 4.9 p~M IBA. Plantlets have been successfully transferred to soil and have been established in the field.

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In Vitro Propagation of Pistacia Species

Cited by 32 publications

References 0 publications

Direct shoot organogenesis from in vitro-derived mature leaf explants of pistachio

Direct shoot organogenesis from in vitro-derived mature leaf explants of pistachio

Combining DOE With Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks in vitro Culture

In vitro propagation of Anacardium occidentale L.

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