In vitro propagation of red squill, Urginea maritima Baker

Abstract

Red squill (Urginea maritima Baker), a plant in the Liliaceae of potential use as a rodenticide, was successfully propagated in vitro. Bulblets were induced from red squill bulb-scales cultured in the dark on a medium containing Murashige and Skoog [6] salts supplemented with a combination of 0.5 or 1.6 #M naphthaleneacetic acid and 0.4 or 1.3/~M 6-benzylaminopurine. Bulblets induced in vitro were rooted in medium containing 0.5 or 1.6/tM naphthaleneacetic acid and planted in vermiculite. Bulb-scale explants could be returned to fresh medium regenerate new bulblets, which were easily rooted. Alternatively. bulblets could be subcultured to regenerate adventitious shoots in medium containing 4.4 or 13.2 pM 6-benzylaminopurine. Shoots regenerated in this manner proved difficult to root by a number of treatments tested. Regenerated bulblets were chilled at 5 C for 3 to 4 weeks to induce leaf emergence prior to transfer to the greenhouse.

In VitroRegeneration of Red SquillUrginea Maritima(L.) Baker. Using Thidiazuron

“…established plants were transferred to fields for flowering and record of morphological observations. only two reports (2,8) are available on in vitro regeneration of U. maritima without mention of axillary or adventitous bulblet regeneration. this study was conducted primarily to generate information on the nature of response exhibited by single and double scales on in vitro regeneration.…”

“…In vitro propagation is an alternative for speedy propagation of plantlets. A very limited literature is available on the micropropagation of Urginea species (2,4,8), which emphasizes the need for further studies.…”

Endogenous cytokinin dynamics in micropropagated tulips during bulb formation process influenced by TDZ and iP pretreatment

“…However, there are conflicting indications of the roles of cytokinins (CKs) in bulb formation in vitro. Benzyladenine (BA) stimulated the bulb development in Lilium longiflorum (Nhut 1998) and Urginea maritima (El Grari and Backhaus 1987) but inhibited bulb formation in Narcissus jonquilla (Chow et al 1992). A high cytokinin to auxin ration improved the bulb production in Hyacinthus (Kim et al 1981) and Fritillaria fischeriana (Mirici et al 2005).…”

Shoot regeneration from nodules of Charybdis sp.: a comparison of semisolid, liquid and temporary immersion culture systems

“…has been performed, e.g. through bulblet induction (El Grari and Backhaus, 1987) or by adventitious shoot formation (Stojakowska, 1993).…”