In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

Abstract

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18.16 μM TDZ with 0.54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91.67 % and a shoot regeneration rate of 5.46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17.17 % with 11.17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5.40 μM NAA was used as a PGR, the maximum root induction rate was 69.45 %. Regenerated plantlets were successfully transferred to soil.

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

“…Here, medium supplemented with IBA showed higher rate of root induction and longer roots than control or medium supplemented with different levels of NAA. In agreement, Prathanturarug et al (2012) found that IBA was superior to NAA in terms of root induction percentage and number of roots per shoot with S. hutanguriana regenerated shoots after 8 weeks in culture. A similar trend was also observed for other species including Centella asiatica (Tiwari et al 2000), Boscia senegalensis (Khalafalla et al 2011), Pappea capensis (Mngomba et al 2007) and Teucrium fruticans (Frabetti et al 2009).…”

In vitro propagation of the elite species plant Pluchea lanceolata: Assessment of genetic stability by random amplified polymorphic DNA (RAPD) analysis

“…However, on IAA amended medium fewer shoots were produced as compared to IBA and NAA. The synergistic effect of TDZ in combination with other auxin has been demonstrated in the case of Stemona hutanguriana (Prathanturarug et al, 2012). By repeated sub-culturing on MS with TDZ (2.83 µM) and NAA (0.1 µM) number of shoots increased (45±1.33) after five times of sub cultures (each of 21 days) beyond which there is gradual decline in multiplication rate.…”