In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies

Yu, Rongpei; Zhang, Guangfei; Han, Lei; Cai, Hua; Mo, Xijun; Gui, Min; Zhou, Xuhong; Ya-lian, Jiang; Li, Shenchong; Wang, Jihua

doi:10.1007/s11240-016-1116-0

Cited by 26 publications

(25 citation statements)

References 32 publications

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“…affinis (Fern andez et al, 1996). The 1/4 MS medium with 0.1% or 0.2% AC promoted the plantlets regenerated from GGBs in Cibotium barometz (Yu et al, 2017b). Our results also confirmed that the low mineral salt concentration and addition of AC were beneficial for plantlet formation in S. pulvinata, and that the largest number of plantlets (11.80 ± 1.28) was obtained from single adventitious shoots cultivated on 1/4 MS medium supplemented with 0.1% (w/v) AC (Table 2).…”

Section: Resultsmentioning

confidence: 95%

“…Effects of cytokinin on the original shoot induction and adventitious shoot proliferation. Cytokinin is often used for GGB induction in fern plants (Amaki and Higuchi, 1991;Higuchi and Amaki, 1989;Yu et al, 2017b) and adventitious shoot induction in other seed plants (Alawaadh et al, 2020). Although S. pulvinata belongs to fern allies, GGBs were not induced by cultivating the sterilized frond tips on the media containing various concentrations of BAP or TDZ.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, the plantlets in plastic pots containing a 5:1 (v/v) mixture of peat and perlite achieved the maximum survival rate (92.13 ± 1.67%) (Table 3); therefore, it was considered the optimal substrate type for plantlet acclimatization. The same type of substrate was also suitable for the plantlet acclimatization of Cibotium barometz (Yu et al, 2017b). Nevertheless, a 3:1 mixture of horticultural substrate and decomposed granite favored the growth of acclimated plantlets in Selaginella martensii (Park et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

Yu¹,

Cheng²,

Pu³

et al. 2021

horts

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The resurrection plant Selaginella pulvinata (Hook. & Grev.) Maxim is used as an ornamental and medicinal plant. It is also a good candidate for exploring the desiccation tolerance of resurrection plants. However, there is not an efficient propagation method for S. pulvinata. In the present study, we evaluated the establishment of in vitro propagation of S. pulvinata using frond tips as explants. The original shoot induction, adventitious shoot proliferation and plantlet growth media, and substrate type of plantlet acclimatization were investigated. The highest induction rate of original shoots (61.77 ± 5.17%) was obtained on half-strength (1/2) MS medium supplemented with 0.1 mg·L−1 N6-benzylaminopurine (BAP). The 1/2 MS with 1.0 mg·L−1 BAP was the most effective medium for the adventitious shoot proliferation. The quarter-strength (1/4) MS containing 0.1% (w/v) active charcoal (AC) was optimum for plantlets proliferated from adventitious shoots and plantlet growth. Approximately 98 plantlets could be obtained from one single original shoot via one-time shoot proliferation cultivation and plantlet cultivation. The acclimated plants on a 5:1 (v/v) mixture of peat and perlite had the highest survival rate (92.13 ± 1.67%). The acclimated plants maintained excellent resurrection ability.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

Yu¹,

Cheng²,

Pu³

et al. 2021

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“…Ferns have a unique propagation system through the formation of a green globular body (GGB), a tissue produced by unlimited cell division of sporophytes in response to cytokinin during in vitro culture [8]. With many meristematic cells inside and/or near its surface, GGB displays a high proliferation rate [9][10][11]; therefore, it has excellent potential for micropropagation and conservation of fern species [8, 12,13].…”

Section: Introductionmentioning

confidence: 99%

Green Globular Body (GGB) Induction and Differentiation in the Medicinal fern Drynaria Roosii

Yang

Dong

et al. 2021

Preprint

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Background: The green globular body (GGB) of ferns is a special propagule induced in plant in vitro culture systems. Owing to its high proliferation efficiency, GGB is widely used in the in vitro propagation of important ornamental and medicinal ferns. In addition, propagation using GGB shows great development prospects in the conservation of rare or endangered ferns and the breeding of new fern varieties. However, due to the lack of systematic studies on GGB ontogenesis, the morphogenetic aspects of GGB during induction and differentiation remain unclear.Results: We characterized the response of five types of explants of Drynaria roosii to GGB inductive medium and further investigate morphological and anatomical changes of explants that developed GGBs. We found that the rhizome explants directly produced GGBs through cell proliferation of the shoot apical meristem and lateral meristem. The leaf and petiole explants produced GGBs indirectly through the proliferation of meristematic cells of somatic embryos derived from the epidermal cells of the explants. The root and gametophyte explants failed to produce GGB under our induction conditions. We further investigated the differentiation process of GGB. During GGB differentiation, shoot primordia and leaf primordia differentiate from meristematic cells on the epidermis, and the root primordia develop from an inner meristematic tissue with developing vascular tissue connecting all these primordia, which indicates the involvement of multiple organogenesis processes.Conclusions: Our results suggested that preexisting or reestablished meristematic cells were the direct source of GGB in D. roosii. Somatic embryogenesis and organogenesis were involved in GGB induction and differentiation, respectively. The comparison with other common propagules revealed that GGB in D. roosii was largely different from somatic embryos, callus, and protocorm or protocorm-like bodies.

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“…Studies dealing with the Cibotium propagation are only a few. Goller and Rybczyński (2007) Yu et al (2016b) reported green globular bodies induction on half-strength MS media supplemented with Thidiazuron and α-Naphthaleneacetic acid (NAA). This paper reports our study on the in vitro propagation of C. barometz from Sumatra.…”

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confidence: 99%

In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

Isnaini

Praptosuwiryo

2020

Biodiversitas

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Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.

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In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies

Cited by 26 publications

References 32 publications

In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants

Green Globular Body (GGB) Induction and Differentiation in the Medicinal fern Drynaria Roosii

In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

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