In vitro propagation of two antidiabetic species known as guarumbo: Cecropia obtusifolia and Cecropia peltata

Nicasio-Torres, Pilar; Erazo-Gómez, Juan Carlos; Cruz-Sosa, Francisco

doi:10.1007/s11738-009-0304-5

Cited by 13 publications

(18 citation statements)

References 24 publications

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“…This specimen was identified (code number 14123) by Abigail Aguilar, Director of the Mexican Institute of Social Security's (IMSSM) medical plants herbarium of Mexico (Nicasio-Torres et al 2009). Seeds were surface sterilized by successive immersion in 70% ethanol for 5 min, which was followed by immersion in a commercial bleach solution (Cloralex Ò :water, 1:5 v/v) for 10 min.…”

Section: Plant Materialsmentioning

confidence: 99%

“…HPLC analyses for CGA (Sigma Chemical Co., St Louis, MO, USA) and ISO (Indofine Chemical Co., Inc., USA) were conducted on a Waters (Separation Module 2695) HPLC with a system controller (Millennium System Manager Software) and under conditions previously established by Nicasio-Torres et al (2009). Samples (60-ll) were eluted onto a Spherisorb ODS-2 column (250 mm 9 4.6 mm, 5 lm) at a 1 ml min -1 flow rate with a binary gradient of mobile phases as follows: (A) H 2 O-(H 3 PO 4 0.5%), and (B) CH 3 CN-CH 3 OH (1:1) within 32 min.…”

Section: Hplc Analysismentioning

confidence: 99%

“…Guarumbo leaves contain phenolic compounds such as chlorogenic acid (CGA) and isoorientin (ISO) (Andrade-Cetto and Wiedenfeld 2001;Nicasio et al 2005;Nicasio-Torres et al 2009); both compounds possess hypoglycemic, hypolipidemic and antioxidant properties (Ko et al 1998;Rodríguez de Sotillo and Hadley 2002). Additionally, it has been reported that CGA stimulates glucose uptake in both insulin-sensitive and -resistant adipocytes (Alonso-Castro et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Changes in biomass allocation and phenolic compounds accumulation due to the effect of light and nitrate supply in Cecropia peltata plants

et al. 2011

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Cecropia peltata is popularly known as ''guarumbo'' in Mexico and is used in traditional medicine for treatment of diabetes mellitus. C. peltata plants were cultivated in a hydroponic system under controlled conditions. Gradients of light (20, 30 and 100 lmol m -2 s -1 ) and nitrate concentrations (13, 2 and 0.2 mM) were applied to estimate their effect on biomass allocation and accumulation of bioactive (chlorogenic acid and isoorientin) phenolic compounds over a 28-day period. According to carbon nutrient balance (CNB) hypothesis predictions, biomass accumulation in foliage was stimulated by the highest irradiance (100 lmol m -2 s -1 ); similarly, at highest irradiance in combination with lowest nitrate concentration (0.2 mM), root growth was stimulated (root-toshoot ratio increased twofold with respect to the control). In these conditions, total phenolics (TP) and chlorogenic acid (CGA) contents were higher in aerial parts than in roots, with a 3.8-fold increase in TP and a 7.7-fold increase in CGA in foliage with respect to the control plants.Isoorientin was accumulated at very low levels. Antioxidant activity and total phenolic content showed a strong positive correlation. Phenylalanine ammonia-lyase activity (PAL) in aerial parts exhibited significant changes ([twofold) by highest irradiance. C. peltata plants allocate biomass and/or phenolic compounds to compensate the oxidative damage (increase in MDA levels) due to changes in light and nitrate restriction. The results are the basis for the establishment of a system of C. peltata culture in view of the potential use of C. peltata in therapeutic preparations for the treatment of diabetes mellitus.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Hplc Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in biomass allocation and phenolic compounds accumulation due to the effect of light and nitrate supply in Cecropia peltata plants

et al. 2011

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“…Many plants are grown under in vitro conditions to conserve the germplasm, examples are micropropagation of Cecropia spp. (Nicasio-Torres et al 2009), Ceropegia intermedia (Karuppusamy et al 2009), Bacopa monnieri (Singh et al 2009), Asparagus racemosus (Bopana and Saxena 2008) and Searsia dentata (Prakash and Van Staden 2008). In some cases, tissue and cell culture is also used for the production of interesting secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of four saponin producing Maesa species

Faizal

Lambert

Foubert

et al. 2011

Plant Cell Tiss Organ Cult

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A successful micropropagation system was developed for four different medicinal Maesa species. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), thidiazuron (TDZ) and/or alpha-naphthalene acetic acid (NAA). The success of regeneration varied for different species and depended on the type and concentration of plant growth regulators. Regenerated shoots spontaneously developed roots within 6 weeks on MS hormone-free medium. The rooted shoots were transferred to the greenhouse with a 100% success rate. Furthermore, flow cytometry analysis indicated that there were no changes in ploidy level of those regenerated shoots as compared with wild type adult plants. Thin layer chromatography (TLC) analysis revealed that common and distinguishing spot of saponins were similarly observed in regenerated shoots compared to the control plants. Therefore, the protocol also provides an effective means for the in vitro conservation of Maesa spp. that produce pharmaceutically interesting saponins

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“…Both of these species have been used for diabetes and other ailments in Latin America and elsewhere, though for C. peltata, its congeneric C. obtusifolia is more widely known and used Ferreira et al 2012;Nicasio-Torres et al 2009;Quanico et al 2008). One study (Nicasio-Torres et al 2009) of the antidiabetic activity of Cecropia species suggested that the phenolics chlorogenic acid and isoorientin that were isolated from their extracts were responsible for their activity.…”

Section: Discussionmentioning

confidence: 99%

Medicinal Plants of Trinidad and Tobago: Selection of Antidiabetic Remedies

Bullard-Roberts¹

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Thanks to my Ethnobotany sisters, Cassy Quave and Janna Rose, for making the path smoother and to the Koptur lab for adopting me when I had no lab family left.Thanks to Sasha, Asha and Xtina from the Jaffe and Berry Labs at BBC for sharing their space, equipment and expertise.Thanks to James and Kate at the Quave Lab at Emory University for helping me to navigate through failed trials and strange calculations. Diabetes mellitus (DM) is a group of non-infectious diseases that cause hyperglycemia. DM symptoms were first clinically described by ancient Greek physicians whose prescriptions included plant-based remedies. Today, DM affects >400 million people globally and prevalence rates are rapidly increasing in developing countries where basic healthcare relies on local knowledge of botanical remedies. Many developing countries are home to diverse peoples and plants-providing fodder for varied plant-selection strategies and unique botanical pharmacopoeias.I addressed the plant-selection strategies used in a multi-ethnic, developing country, Trinidad and Tobago (T&T), to ascertain their role in shaping the local antidiabetic pharmacopoeia and to assess their benefits and risks in identifying safe and useful remedies. Using literature reviews, field surveys, and laboratory bioassays, Icompleted three categories of analysis.Ethnobotanical analyses showed that T&T's antidiabetic pharmacopoeia is primarily of recent origin as >50% of the 48 historical DM remedies were Neotropical natives, including congenerics of well-known medicinal Paleotropical genera. ix Nevertheless, conservative knowledge transmission was also evident as several Paleotropical species of T&T's pharmacopoeia, including Momordica charantia and Catharanthus roseus were also used in Africa, India and across the Caribbean.Paleotropical natives with a long history of use are likely to be safer remedies.Ethno-medicinal analyses of the pre-and post-2000 DM remedies of T&T, totaling 99 species, suggest that the centuries-old hot/cold folk disease-model was the model predominantly used in plant-selection. Parallels found between T&T folk concepts and biomedical mechanisms of DM provide probable bases for efficacy but the chronic use of purgatives and bitter-tasting plants is likely to be risky.Phytochemical analyses revealed that 69% of the tested plant extracts contained phenolic compounds, with more than half producing >80% alpha-glucosidase inhibition.Phenolic content and alpha-glucosidase inhibition were strongly correlated among food plants used as medicines, suggesting higher probability of selection as a result of nontarget effects. The medicinal use of food plants may provide the best margins of safety and efficacy in identifying antidiabetic remedies.Together, these analyses showed how culture-specific plant-selection strategies can identify safe, useful remedies for developing countries to address their increasing DM prevalence in a cost-effective and sustainable manner.x Table 3 Regions of the Indian sub-continent from which indentured laborers...

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In vitro propagation of two antidiabetic species known as guarumbo: Cecropia obtusifolia and Cecropia peltata

Cited by 13 publications

References 24 publications

Changes in biomass allocation and phenolic compounds accumulation due to the effect of light and nitrate supply in Cecropia peltata plants

Changes in biomass allocation and phenolic compounds accumulation due to the effect of light and nitrate supply in Cecropia peltata plants

In vitro propagation of four saponin producing Maesa species

Medicinal Plants of Trinidad and Tobago: Selection of Antidiabetic Remedies

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