In vitro propagation of wild yams, Dioscorea oppositifolia (Linn) and Dioscorea pentaphylla (Linn)

Poornima, G; Ravishankar, Rai V

doi:10.5897/ajb2007.000-2368

Cited by 33 publications

(29 citation statements)

References 27 publications

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“…According to Poornima & Ravishankar (2007), auxins may have a negative effect on in vitro adventitious root growth, when plants remain in their presence all the time. This was not observed for the employed IBA and NAA concentrations in N. concentrica.…”

Section: Resultsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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The tissue culture can contribute to the propagation of several economic species, such as the bromeliads. This research aimed at evaluating the auxins type and concentration in the in vitro and ex vitro rhizogenesis of Neoregelia concentrica bromeliad. N. concentrica shoots were induced in a growth medium with 15.0 µM of 6-benzylaminopurine, for 80 days, followed by sub-cultivation in phytoregulator-free medium, for 45 days. In the in vitro rhizogenesis, the shoots grew in a medium supplemented with indole-3-butyric acid (IBA) or naphthalene-acetic acid (NAA), at the concentrations of 0.0 µM, 1.0 µM, 2.0 µM, 3.0 µM and 4.0 µM. In the ex vitro rhizogenesis, the bases of shoots were immersed, for 60 minutes, in IBA or NAA solutions, at the concentrations of 0.0 µM, 5.0 µM, 10.0 µM and 15.0 µM. After immersion, the shoots were planted in plastic trays with vermiculite. At the end of each rhizogenesis method, the phytotechnical parameters analysis was carried out. For the in vitro rhizogenesis, a higher number of roots were observed when the shoots were cultivated in concentrations higher than 1.0 µM of NAA, when compared to the IBA. However, the rooting rate differed only at 30 days after the in vitro growth, with a higher root induction in the shoots grown with NAA. At 60 days, the rooting rate was higher than 90% and statistically similar in all treatments. In the ex vitro rhizogenesis, a better formation of the rooting system was observed when 5.0 µM of IBA was applied, with higher rooting averages and number of roots.

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Section: Resultsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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“…Besides its importance as a staple food, yam is also a source of income for people, particularly in the coastal region of West Africa (Asiedu and Sartie 2010). A number of Dioscorea species are also the source of phyto-compounds which are used in the production of corticosteroids and have potential uses as excipients in the pharmaceutical industry (Poornima and Ravishankar 2007;Zuluaga et al 2007;Okunlola and Odeku 2011). Yam production has been constrained owing to several abiotic and biotic factors that include diseases and pests, inadequate planting material, low yield potential, and decreasing soil fertility (Amusa et al 2003;Adegbite et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5-12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.

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“…However, the growth rate declined over time, and by week 8, it was at the maximum (0.31 shoots formed per week) at 10 µM kinetin. Poornima and Ravishankar [23] studied kinetin effects on two wild yams-D. oppostifolia and D. pentaphylla, and showed an average of 1.5 shoots per plantlet at 2.3 µM kinetin and 3.4 shoots per plantlet at 9.3 µM kinetin for D. oppostifolia. However, the authors observed no effects of kinetin on D. pentaphylla, with an average of 1.4 shoots per plantlet.…”

Section: Discussionmentioning

confidence: 99%

“…Kinetin is often reported to have significant effects on shoot formation and very little effects on root formation; however, a number of studies have also observed significant effects of kinetin on root formation, as well as varying effects on the shoot formation of D. alata [3,[23][24][25][26]. Here, surface response models were developed to better evaluate the effects of kinetin on D. alata propagated in vitro.…”

Section: Using Surface Response Models To Evaluate the Effects Of Kinmentioning

confidence: 99%

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Using Surface Response Models to Evaluate the Effects of Kinetin on Dioscorea alata Propagated in Vitro

Nelimor¹,

Sintim²,

Kena³

et al. 2017

JAST-B

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Abstract:Kinetin is an important growth hormone used for in vitro propagation, but its dynamic and temporal effects on Dioscorea alata have not been thoroughly evaluated. In this study, surface response models were developed to better elucidate the effects of kinetin on D. alata propagated in vitro. Nodal segments were obtained from Akaaba, an important D. alata cultivar in Ghana, and propagated in vitro under five kinetin rates (0, 2.5, 5, 7.5 and 10 µM). The models were developed using segmented multiple regression with time and kinetin as the predictors. The effects on plant height, the number of leaves, shoots and roots were assessed with three-dimensional figures for better observation of temporal trends. The model fit was very good with normalized root mean squared error (NRMSE) = 0.1, R-squared = 0.83 and adjusted R-squared = 0.82, averaged across the different growth parameters. Different kinetin levels elicited the maximum shoot, leaf and root formation, as well as the growth rates over time. Moderate kinetin levels (2-4 µM) provided better growth at early culturing period. Higher kinetin levels (5-10 µM) suppressed the growth of the plantlets at early stages, but the plantlets recovered from the stress and resumed normal growth thereafter. After 4-5 weeks, the growth rates of the moderate kinetin levels (2-4 µM) declined much faster and were lower compared to the higher kinetin levels, except plant height and the number of roots which were still higher at the moderate kinetin level even after eight weeks of culturing. Thus, kinetin requirements vary depending on the growth parameters of interest.

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In vitro propagation of wild yams, Dioscorea oppositifolia (Linn) and Dioscorea pentaphylla (Linn)

Cited by 33 publications

References 27 publications

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Using Surface Response Models to Evaluate the Effects of Kinetin on Dioscorea alata Propagated in Vitro

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