In vitro propagation via organogenesis and embryogenesis of Cyrtanthus mackenii: a valuable threatened medicinal plant

Kumari, Aloka; Baskaran, P.; Staden, J. Van

doi:10.1007/s11240-017-1293-5

Cited by 15 publications

(8 citation statements)

References 22 publications

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“…Unfortunately, we did not observe SELSs germinated with distinct root system. The founding of approaches for somatic embryogenesis for in vitro mass propagating techniques and for conserving medicinally significant and endangered plants emerge as a vital and promising offshoot protocol [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Yang

et al. 2021

Plants

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This work was aimed at establishing an effective approach for in vitro propagation of Ajuga lupulina Maxim, a medicinal and ornamental plant mainly found in eastern Xizang, in the western Sichuan region of China. We report an optimum response in the proliferation of axillary shoots from nodal segment explants (10.2 shoots/explant) on MS medium containing 3.0 mg L−1 of 6-benzyladenine (BA). When BA and TDZ individually or in combination with NAA were employed for adventitious shoot regeneration, shoots and embryo-like structures (ELSs) were noted in the callus from leaf explants. The maximum response of 26.4 shoots /explant (81.6%) and 12.0 ELSs/explant were ascertained on MS medium with 4.0 mg L−1 TDZ and 0.1 mg L−1 NAA. The leaf despite browning still demonstrated a high regeneration capacity. TDZ (2.0 mg L−1) and BA (2.0 mg L−1) along with NAA (0.01 mg L−1) were found to perform well for shoot regeneration via callus from shoot tip explants. The best for rooting was MS medium (half-strength) containing indole-3-butyric acid (IBA: 1.5 mg L−1) and (NAA: 0.5 mg L−1) with the maximum number of roots (25.8 per shoot) and the highest rooting frequency (81.71%). The survival of the plantlets in the greenhouse was 78.2% indicative of successful acclimatization. This work is the first report of a consistent, definitive, and unique protocol for A. lupulina regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.

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Section: Discussionmentioning

confidence: 99%

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Yang

et al. 2021

Plants

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“…The results of the current research revealed that the highest numbers of embryos directly regenerated on initial explants were recorded for 2,4-D or NAA combined with TDZ while auxins alone purely induced SE. Furthermore, we observed lower numbers of tulip embryos in the presence of picloram, even when it was combined with TDZ, unlike in other species such as Lachenalia viridiflora [31], Cyrtanthus mackenii [32], Manihot esculenta [33] and Lilium sp. [34].…”

Section: Se Induction and Embryo Developmentmentioning

confidence: 72%

Micropropagation of Tulip via Somatic Embryogenesis

Podwyszyńska

Marasek-Ciołakowska

2020

Agronomy

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An effective method of tulip regeneration via somatic embryogenesis (SE) was developed. Explants, flower stem slices excised from cooled bulbs were incubated in darkness on MS modified media containing auxins alone (2,4-dichlorophenoxyacetic acid—2,4-D, 1-naphthalene acetic acid—NAA and 4-amino-3,5,6-trichloro-2-pyridine carboxylic acid—picloram) or combined with thidiazuron (TDZ) at 0.1 and 0.5 mg L−1. Yellowish-white callus with a granular structure was developed in the presence of all auxins on the cut surface from the tissues of the vascular bundles. From this, lines of embryogenic calli were derived. The addition of TDZ to the medium with auxins significantly stimulated somatic embryo formation. Cyclic and the most intensive proliferation of embryogenic callus as well as embryo formation was obtained in the presence of 2,4-D at 0.1 mg L−1 combined with TDZ at 0.5 mg L−1. Addition of proline enhanced either callus proliferation rate or frequency of embryo formation. The best quality embryos with cotyledons longer than 10 mm able to form bulbs were recorded when TDZ was replaced with 6-benzylaminopurine (BAP) at the concentration of 0.1 mg L−1. Histomorphology showed that the development of somatic embryos could have either external or internal origins. Embryos of external origin were initiated by cell division on the edge of embryogenic calli. Embryos of internal origin resulted from the division of parenchyma cells inside the tissue. Embryonic cells were characterized by their small volume, regular shape, dense cytoplasm and large nuclei. The globular embryos were covered by a distinct layer of periderm. Then, the embryos developed into structures having leaf-shaped cotyledons with a procambial strand and a sideward-orientated meristem of the vegetative apex (stolon). Cotyledon embryos did not show vascular connections with the parent tissue, and they did not develop embryonic roots.

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“…It allows the sustainable production of thousands of plant species in a short period of time. Attempts have been made for the conservation of different African medicinal plants via in vitro regeneration methods [13][14][15][16][17][18].…”

Section: Micropropagationmentioning

confidence: 99%

“…In vitro propagation through organogenesis and conservation strategies for Cyranthus mackneii have been reported [16]. MS [19] medium supplemented with increased amount of sucrose (40 g L −1 ), 10 µM picloram, 20 µM N 6 benzyladenine (BA) and 20 µM glutamine produced a maximum number of shoots in a short period of time.…”

Section: Micropropagationmentioning

confidence: 99%

Conservation Strategy for African Medicinal Species: In Vitro Biotechnological Approach

Jha¹,

Chahal²,

Pandey³

et al. 2020

Phyton

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The use of medicinal plants for different therapeutic values is well documented in African continent. African diverse biodiversity hotspots provide a wide range of endemic species, which ensures a potential medicinal value. The feasible conservation approach and sustainable harvesting for the medicinal species remains a huge challenge. However, conservation approach through different biotechnological tools such as micropropagation, somatic embryogenesis, synthetic seed production, hairy root culture, molecular markers based study and cryopreservation of endemic African medicinal species is much crucial. In this review, an attempt has been made to provide different in vitro biotechnological approaches for the conservation of African medicinal species. The present review will be helpful in further technology development and deciding the priorities at decision-making levels for in vitro conservation and sustainable use of African medicinal species

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In vitro propagation via organogenesis and embryogenesis of Cyrtanthus mackenii: a valuable threatened medicinal plant

Cited by 15 publications

References 22 publications

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Micropropagation of Tulip via Somatic Embryogenesis

Conservation Strategy for African Medicinal Species: In Vitro Biotechnological Approach

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