In vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin

Ercan, Ertuğrul; Bağla, Aysel Güven; Aksoy, Aziz; Gacar, Gülçin; Ünal, Zehra Seda; Aşgün, Halil Fatih; Karaöz, Erdal

doi:10.1016/j.acthis.2013.06.007

Cited by 11 publications

(4 citation statements)

References 32 publications

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“…Several studies have addressed the effects of hypoxia on MSCs. The findings have been equivocal [ 10 11 12 ]. Some reports demonstrated that human bone marrow-derived MSCs cultured under hypoxia showed a diminished capacity to differentiate into adipocytes and osteocytes, supporting the notion that low oxygen tension promotes an undifferentiated state [ 13 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of CoCl₂on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Yoo

Moon

Kim

2016

Korean J Physiol Pharmacol

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Mesenchymal stem cells (MSCs) in the bone marrow and other somatic tissues reside in an environment with relative low oxygen tension. Cobalt chloride (CoCl2) can mimic hypoxic conditions through transcriptional changes of some genes including hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF). This study evaluated the potential role of CoCl2 preconditioning on multi-lineage differentiation of C3H/10T1/2, a murine MSC line to understand its possible molecular mechanisms in vitro. CoCl2 treatment of MSCs markedly increased HIF-1α and VEGF mRNA, and protein expression of HIF-1α. Temporary preconditioning of MSCs with CoCl2 induced up-regulation of osteogenic markers including alkaline phosphatase, osteocalcin, and type I collagen during osteogenic differentiation, followed by enhanced mineralization. CoCl2 also increased chondrogenic markers including aggrecan, sox9, and type II collagen, and promoted chondrocyte differentiation. CoCl2 suppressed the expression of adipogenic markers including PPARγ, aP2, and C/EBPα, and inhibited adipogenesis. Temporary preconditioning with CoCl2 could affect the multi-lineage differentiation of MSCs.

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Section: Introductionmentioning

confidence: 99%

Effects of CoCl₂on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Yoo

Moon

Kim

2016

Korean J Physiol Pharmacol

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“…Additionally, EPO can interact with MSCs to promote the secretion of microvesicles for stronger paracrine wound healing (Wang et al, 2015). It also confers a protective effect on these cells in the harsh microenvironment of wounds, thereby improving graft survivability (Ercan et al, 2014;Ding et al, 2018). As such, we sought to genetically enhance MSCs by combining them with EPO as a single treatment via genetic modification.…”

Section: Introductionmentioning

confidence: 99%

Transplanted Erythropoietin-Expressing Mesenchymal Stem Cells Promote Pro-survival Gene Expression and Protect Photoreceptors From Sodium Iodate-Induced Cytotoxicity in a Retinal Degeneration Model

Koh

Alsaeedi

Rashid

et al. 2021

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSC) are highly regarded as a potential treatment for retinal degenerative disorders like retinitis pigmentosa and age-related macular degeneration. However, donor cell heterogeneity and inconsistent protocols for transplantation have led to varied outcomes in clinical trials. We previously showed that genetically-modifying MSCs to express erythropoietin (MSCEPO) improved its regenerative capabilities in vitro. Hence, in this study, we sought to prove its potential in vivo by transplanting MSCsEPO in a rat retinal degeneration model and analyzing its retinal transcriptome using RNA-Seq. Firstly, MSCsEPO were cultured and expanded before being intravitreally transplanted into the sodium iodate-induced model. After the procedure, electroretinography (ERG) was performed bi-weekly for 30 days. Histological analyses were performed after the ERG assessment. The retina was then harvested for RNA extraction. After mRNA-enrichment and library preparation, paired-end RNA-Seq was performed. Salmon and DESeq2 were used to process the output files. The generated dataset was then analyzed using over-representation (ORA), functional enrichment (GSEA), and pathway topology analysis tools (SPIA) to identify enrichment of key pathways in the experimental groups. The results showed that the MSCEPO-treated group had detectable ERG waves (P <0.05), which were indicative of successful phototransduction. The stem cells were also successfully detected by immunohistochemistry 30 days after intravitreal transplantation. An initial over-representation analysis revealed a snapshot of immune-related pathways in all the groups but was mainly overexpressed in the MSC group. A subsequent GSEA and SPIA analysis later revealed enrichment in a large number of biological processes including phototransduction, regeneration, and cell death (Padj <0.05). Based on these pathways, a set of pro-survival gene expressions were extracted and tabulated. This study provided an in-depth transcriptomic analysis on the MSCEPO-treated retinal degeneration model as well as a profile of pro-survival genes that can be used as candidates for further genetic enhancement studies on stem cells.

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“…В частности, окислительный стресс значительно снижает жизнеспособность клеток, а одним из веществ, способных влиять на жизнеспособность клеток, является эритропоэтин [9]. Показано, что эритропоэтин проявляет антигипоксическое и антиапоптотическое действие, усиливает неоваскулогенез, мобилизирует эндотелиальные прогениторные клетки и усиливает ангиогенез [3,8]. Влияние эритропоэтина опосредуется его взаимодействием с рецептором для эритропоэтина, сформировавшийся комплекс эритропоэтин/рецептор к эритропоэтину запускает каскад сигнальных путей с вовлечением янус-киназы (JAK-2), сигнального проводника и активатора транскрипции (STAT) и других сигнальных путей, вовлеченных в регуляцию пролиферации, выживаемости и экспрессии генов [11].…”

Section: Introductionunclassified

Effect of Erythropoietin on Cytokine Production by Stem Cells

et al. 2019

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Erythropoietin (EPO) is mainly used to stimulate erythropoiesis. Its cytoprotective effects upon other cells of the human body and animals were recently shown, in particular, anti-apoptotic effect was observed. EPO effect upon the cells is mediated by interaction with erythropoietin receptor, with a complex forming a heterodimeric bond with β-common chain (CD131). In the present work, we studied the changes in erythropoietin receptor expression, and production spectrum of biologically active molecules in bone marrow mononuclear cells (BM-MNC) of patients with coronary heart disease. The flow cytometric assays showed that short-term incubation of BM-MNC with erythropoietin caused increased expression of the erythropoietin receptors on hematopoietic stem cells and tended to reduce the number of endothelial progenitor cells carrying the erythropoietin receptors. Solid-phase enzyme immunoassay in conditioned media from BM-MNC revealed that long-term (72 hours) exposure of BM-MNC to erythropoietin promoted increased production of IL-1β, PDGF-AB, and Epo, if compared to the basal production level (p < 0.05). Short-term incubation of BM-MNC with erythropoietin (60 minutes) caused a significant increase in the IL-1β, PDGF-AB and CXCL-12 / SDF-1α production levels, as well as significant reduction in the IL-10 production levels compared to the basal levels (p < 0.05).

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In vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin

Cited by 11 publications

References 32 publications

Effects of CoCl₂on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Effects of CoCl₂on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Transplanted Erythropoietin-Expressing Mesenchymal Stem Cells Promote Pro-survival Gene Expression and Protect Photoreceptors From Sodium Iodate-Induced Cytotoxicity in a Retinal Degeneration Model

Effect of Erythropoietin on Cytokine Production by Stem Cells

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In vitro protection of adipose tissue-derived mesenchymal stem cells by erythropoietin

Cited by 11 publications

References 32 publications

Effects of CoCl2on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Effects of CoCl2on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Transplanted Erythropoietin-Expressing Mesenchymal Stem Cells Promote Pro-survival Gene Expression and Protect Photoreceptors From Sodium Iodate-Induced Cytotoxicity in a Retinal Degeneration Model

Effect of Erythropoietin on Cytokine Production by Stem Cells

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Effects of CoCl₂on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells

Effects of CoCl₂on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells