In vitro regeneration and callogenesis in tissue culture of floral organs of the genus Iris (Iridaceae)

Abstract

We tested the differentiation and morphogenetic capacity of floral organs of Iris ensata, I. setosa , and I. sanguinea cultured in vitro . Organogenesis through direct formation of shoots from explants, callogenesis, and floral organogenesis were demonstrated in I. ensata callus culture in vitro . These processes depended on the plant species and on the content of phytohormones in the medium. Adventitious shoots proved to develop on the basal part of the perianth tube and on the apical part of the ovary, while roots were not formed. Direct organogenesis was induced by the following phytohormones: α -naphthylacetic acid and 6-benzylaminopurine for I. ensata and 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine for I. setosa and I. sanguinea ; while callogenesis was induced by 2,4-dichlorophenoxyacetic acid. The obtained data indicate that development of adventitious structures from iris floral organs requires the presence of 6-benzylaminopurine in the growth medium. PLANT PHYSIOLOGYŠ š

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

“…In this study, the combination of 2,4-D with BAP stimulated shoot induction (Figure 1b). Our results agree with those reported by Boltenkov and Zarembo (2005) in Iris ensata, and Anbari et al (2007) in Narcissus papyraceus, who reported that combinations of 2,4-D and BAP were necessary for shoot induction.…”

In vitro rooting of Crocosmia × crocosmiiflora ‘Lucifer’

“…However, in Crocosmia single shoots were rooted on a PGR-free MS medium (Krupa-Małkiewicz et al 2013). Similar results were obtained by Boltenkov et al (2005) for Iris, Koetle et al (2010) for Dierama erectum Hilliard, and Hannweg et al (2013) for Crocosmia aurea. The occurrence of root development on a PGR-free medium may depend on the presence of endogenous auxins in regenerated cormlets.…”

“…Due to their high commercial potential, this group of plants is used for further horticultural development. Many plant breeders throughout the world have conducted research on the Watsonia species, Iris, Crocosmia × crocosmiiflora, or Crocosmia aurea (Niederwieser et al 2002, Boltenkov and Zarembo 2005, Ascough et al 2007, Koh et al 2007, Hannweg et al 2013, Krupa-Małkiewicz et al 2013. Crocosmia × crocosmiiflora, whose natural habitat is in South Africa, are valuable ornamental plants that produce very decorative and attractive flowers and leaves.…”

Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

“…In treatments including BAP, shoot regeneration response changed with species and I. schachtii was found to be better than I. sari. The promotive effect of BAP alone or in combination with NAA-inducing callus and adventitious bulblet regeneration was previously reported in other Iris species (Kawase et al, 1995;Suzhen et al, 1999;Shibli and Ajlouni, 2000;Boltenkov and Zarembo, 2005;Boltenkov et al, 2007).…”

“…This variation may stem from genotype and concentration or combination of PGRs. Boltenkov and Zarembo (2005) reported that plant species and PGRs play an important role in the establishment of regeneration. In treatments including BAP, shoot regeneration response changed with species and I. schachtii was found to be better than I. sari.…”