In vitro Regeneration and Conservation of Rare Medicinal Plant Dregea volubilis Benth.

Kaur

Physiol Mol Biol Plants

2014

Micropropagation offers a great potential to produce millions of clonal individuals through tissue culture via induction of morphogenesis. The aim of this work was to obtain an efficient protocol for callus regeneration for Gentiana kurroo Royle. The morphogenic response of different explants (leaves, petioles, roots) varied and responded differently for regeneration according to combinations of growth regulators. The petiole explants were best responding for callus induction and subsequently for indirect and direct regeneration. The callus induction was achieved on MS basal + 1.0 mg/l benzyladenine (BA) and 3.00 mg/l naphthalene acetic acid (NAA). MS medium supplemented with 0.10 mg/l NAA and 1.0 mg/l thidiazuron (TDZ) was recorded as the best medium for indirect regeneration. However, for direct regeneration the maximum number of shoot emergence was observed on MS basal fortified with 0.10 mg/l NAA + 0.75 mg/l TDZ. Half strength MS basal supplemented with indole-3-butyric acid (IBA) 1.00 mg/l gave best response for root induction. Subsequently, the plantlets were transferred and 100 % survival rate was recorded only on autoclaved cocopeat. No morphological variations were recorded in the callus regenerated plantlets.

Section: Discussionmentioning

confidence: 91%

In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant

Kaur

Physiol Mol Biol Plants

2014

“…1-A gene of A. hydrophila isolates for 55 min at 100 Volt. Lane 1 Marker (1kb DNA ladder) Lane 5,6,7,8,9,11,12,13 were positive result isolates of A. hydrophila while 2,3,4 and 10 were negative result.…”

Section: Isolation and Identification Of A Hydrophilamentioning

confidence: 99%

“…The PCR assay was performed to detect the (16S rRNA) gene for confirmation the identification of A. hydrophila and to detect the virulence factors genes, shown in [Table -1]. These primers synthesized by Alpha DNA company, Canada [11,12]. Concentration of DNA was determined spectrophotometrically by measuring its optical density at 260 nm (Extinction coefficient of dsDNA is 50 µg/ml at 260 nm) the purity of DNA solution is indicated by ratio of OD260/OD280 which is in the range of 1.8± 0.2 for pure DNA [13].…”

Section: Molecular Study Of Aeromonas Hydrophila Isolated From Stool Samples In Najaf (Iraq)mentioning

confidence: 99%

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MOLECULAR STUDY OF Aeromonas hydrophila ISOLATED FROM STOOL SAMPLES IN NAJAF (IRAQ)

AL-Fatlawy¹,

Al-Ammar²

2013

Int J of Micr Res

The present study included the detection of A. hydrophila in some clinical cases in the governorate of AL-Najaf, during the period from October 2011 to March 2012.The samples was collected from stool samples for A. hydrophila isolates. The PCR method of gene (16S rRNA) was the best methods for diagnosis, which has led to isolate and diagnosis of (23) isolate of A. hydrophila from stool samples. The PCR technique was used for detection of some genes in A. hydrophila like heamolysin (hyl) and aerolysin (aerA) genes which were responsible for pathogenicity of bacteria. The (hyl)and (aerA) genes presented in (80%, 72%) respectively for the clinical isolates

“…In previous report, we have described the effect of explants source on axillary shoot multiplication of W. volubilis using nodal explants (Chakradhar and Pullaiah, 2006). Other regeneration protocols also reported nodal explants in W. volubilis (Yogananth et al, 2011;Vinothkumar et al, 2011). However, there has been no report on regeneration of W. volubilis through organogenesis and the present study describes a reproducible, efficient protocol of regeneration system by adventitious shoots using immature leaf explants.…”

Section: Introductionmentioning

confidence: 67%

In vitro regeneration through adventitious buds in Wattakaka volubilis, a rare medicinal plant

Chakradhar¹,

Pullaiah²

2014

Afr. J. Biotechnol.

High frequency shoot regeneration from in vitro derived leaf explants of Wattakaka volubilis (L.f.) Stapf was achieved through callus mediated organogenesis. Organogenic calli were induced from 20 day old aseptic seedling explants on Murashige and Skoog medium fortified with various concentrations and combinations of plant growth regulators, benzylaminopurine (BAP), α-naphthaleneacetic acid (NAA), indole 3-butyric acid (IBA) and gibberellic acid (GA 3). A mean of 8.6 shoots developed from organogenic callus induced from a 2 x 2 cm leaf explants on MS medium with 3% sucrose having 5.37µM NAA in combination with 2.22 µM BAP with 60% induction capacity. Further development of adventitious shoots could be achieved by sub culturing the callus to the same medium with 4.40 µM BAP and 0.288 µM GA 3. Organogenesis could not be achieved from the calli of ex vitro derived leaf explants. The developed shoots were rooted on half-strength MS medium with 1% sucrose, 4.90 µM IBA and 0.93 µM kinetin at a frequency of 85%. Well rooted plantlets were then transplanted to vermiculite soil (3:1) mixture in polythene covered pots kept under culture room conditions. Approximately 60% plantlets survived and grew into whole plants.