In vitro Regeneration of Gerbera jamesonii Bolus from Leaf and Petiole Explants

Kumar, Surinder; Kanwar, J. K.; Sharma, D. R.

doi:10.1007/bf03263196

Cited by 23 publications

(20 citation statements)

References 7 publications

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“…However, the naturally occurring hormones in periwinkle tissues were not able to induce shoot formation. Addition of exogenous PGRs to the medium was necessary for plant regeneration of periwinkle as was found in other plant species such as chokecherry, black cherry, and gerbera (Dai et al, 2004;Espinosa et al, 2006;Kumar et al, 2004). Plant regeneration is a challenge for Catharanthus roseus, especially through organogenesis using vegetative tissues.…”

Section: Discussionmentioning

confidence: 99%

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

Swanberg¹,

Dai²

2008

horts

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Two periwinkle cultivars, Pacific Coral (P1) and Sunstorm Rose (P2), were used for development of a plant regeneration system. Leaf and internodal explants collected from in vitro plants were plated onto woody plant medium (WPM) using a factorial arrangement of 6-benzyladeine (BA) and 1-naphthalene acetic acid (NAA). Shoots were successfully regenerated. Shoot production from leaf tissues was minimal for all cultivars, whereas internodal tissues showed variable rates of regeneration depending on the hormone combination. Cultivar P1 showed the maximum regeneration rate (73.3%) when internodal explants, 4 to 6 mm in length, were placed on WPM containing 5 μm BA and 5 μm NAA. Cultivar P2 showed a regeneration rate of 56.7% with a combination of 20 μm BA and 10 μm NAA. Shoot regeneration rate increased as the internodal explant size increased for P2; however, the regeneration rate decreased when the explant size was greater than 7 mm for P1. The shoot regeneration rate decreased as the period of the dark treatment of internodal explants increased in both P1 and P2. The antibiotics carbenicillin (Carb) and cefotaxime (Cef) had little effect on shoot regeneration. There was a slightly higher rate observed for P1 when Cef was added into the medium, whereas P2 showed a decrease with the addition of Cef. Carb showed no significant effect on shoot regeneration for both cultivars. Addition of both Carb and Cef to the medium slightly inhibited shoot regeneration.

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Section: Discussionmentioning

confidence: 99%

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

Swanberg¹,

Dai²

2008

horts

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“…Rademaker (1990) reported that cultivar and explant type had a greater effect on regeneration than the type of medium. Addition of exogenous growth regulators to the medium was necessary for plant regeneration of periwinkle has been found in other plant species such as chokecherry, black cherry, and gerbera (Dai et al, 2004;Kumar et al, 2004;Espinosa et al, 2006) and in periwinkle (Swanberg and Dai, 2008).…”

Section: Shoot Formationmentioning

confidence: 92%

In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants

2013

Alexandria Science Exchange Journal: An International Quarterly

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“…Direct shoot regeneration occurred during the first four weeks and the effectiveness of shoot regeneration depended on the cultivar, the sequence of passage on regeneration medium, growth regulators and duration of the induction period. Xu et al (2002) and Kumar et al (2004) regenerated adventitious shoots from petiole and leaf pieces of G. jamesonii in a medium supplemented with different concentrations of auxins and cytokinins.…”

Section: Leaf and Petiolementioning

confidence: 99%

“…An unknown phytotoxic component was found under normal cultural conditions only in PP containers; it caused severe loss of quality in plants grown in PP contain-ers. Kumar et al (2004) reported 60-70% success in pots containing a mixture of sand:farmyard manure mixed in the ratio 1:1. Kaur et al (1999) obtained 100% survival rate of in vitro shoots when transferred to pots filled with a mixture of soil:sand:compost in 1:1:1 ratio.…”

Section: Greenhouse Acclimatizationmentioning

confidence: 99%

In vitro propagation of Gerbera: A Review

Kanwar¹,

Kumar²

2008

Hort. Sci. (Prague)

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Gerbera has gained popularity in the past few years in many countries of the world and it is in great demand in the floral industry as cut flower as well as potted plant due to its beauty, colour, long vase life, and ability to rehydrate after long transportation. The most commercial cultivars are propagated through vegetative means by multiplication through divisions of clumps; however, the multiplication by this method is too slow to be commercially viable. To commercialize this crop and to meet the growing demand for planting material, tissue and organ culture techniques are being used as alternative methods for propagation in many countries. Most of the work has been carried on plant regeneration by adventitious organogenesis from capitulum, shoot tip, leaf, petiole and other parts of the plant. Attention should be paid to improve the technology to achieve 100% success in all species/cultivars to meet growing demands of the growers globally. From the literature, it is evident that gerberas are highly amenable to in vitro studies, as various explants were found to favourably respond to different culture media with different types and concentrations of growth regulators.

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In vitro Regeneration of Gerbera jamesonii Bolus from Leaf and Petiole Explants

Cited by 23 publications

References 7 publications

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis

In Vitro Regeneration and Somaclonal Variation of Catharanthus roseus Don. Using Leaf and Internodal Explants

In vitro propagation of Gerbera: A Review

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