In Vitro Regeneration of High Yielding Indica Rice (Oryza sativa L.) Varieties

Abstract

The experiment was conducted to observe the regeneration potential and also to establish a suitable in vitro plantlet regeneration protocol from mature seed derived embryogenic calli of four indica rice varieties viz BRRI dhan28, BRRI dhan29, BRRI dhan47 and Binadhan-7 after partial desiccation treatment. Different concentrations and combinations of growth regulators were used in MS medium to observe the callus induction ability using mature embryo as explants. The percentage of callus induction frequency was highest (86.00) in BRRI dhan47 and the lowest (56.50) in Binadhan-7 Among the culture media the performance of MS +500 mgl -1 L-Proline + 2.0 mglBAP was better than any other media for callus induction frequency (%), rapid callusing, size of the callus (mm), texture of callus and color of callus. Among the four varieties, shoot regeneration was highest in BRRI dhan29 (84.33%) which required minimum (14.80) days to and the lowest was in Binadhan-7 (39.67%) which required maximum (15.47) days. Among the treatments, the highest (65.75%) shoot regeneration was observed with MS + 6.0 mgl -1 Kn + 0.5 mgl -1 NAA which required minimum days (13.75) to develop green bud formation and the lowest shoot regeneration (56.50) was observed with MS + 2.0 mgl -1 Kn + .05 mgl -1 NAA. BRRI dhan29 produced more number of shoots (4.67) per callus while Binadhan-7 showed minimum number of shoots (2.87) per callus. The highest number of shoot producing roots (3.66) was observed in BRRI dhan29 which showed maximum number of root per plant and the lowest (3.11) in Binadhan-7. Among the three treatments MS + 0.6 mgl -1 IBA showed highest percentage 86.67 of root followed by MS+ 0.6 mgl IBA. The establishment rate of the plantlet in the pot was the highest (67.67%) in BRRI dhan29 and Binadhan-7 showed lowest establishment rate (51.22%) in pot.

Natural and Induced Glufosinate-resistance in selected Rice (<em>Oryza sativa L</em>.) Varieties in Sri Lanka

“…Results indicated that 2 mgl -1 2,4-D with 0.1mgl -1 Kinetin or BAP was the most appropriate combination for callus induction. This fi nding agrees with the previous studies documented by Revathi et al, (2011), Alam et al, (2012), Dahanayake and Ranawake, (2012), Mannan et al,(2013), Dissanayaka and Dahanayaka, (2014), and Upadhya et al, (2015). The Journal of Agricultural Sciences -Sri Lanka , 2019, Vol.14, No.…”

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

“…30 Approximately similar increment of regeneration was recorded in maize, 58,59 sugarcane 35 and rice. 29,60,61 Under this study we have recorded around 2-3 folds higher regeneration from desiccated calli in BR10, BRRI dhan32 and BRRI dhan47 (Fig. 4, Table 3).…”

“…27,28 An efficient plant regeneration in Bangladeshi indica rice is still poses a major problem for genetic manipulation through innovative approaches. 14,29 The optimal desiccation periods were 72 h and 48 h for Malaysian rice cultivars of MR232 and MR220 respectively, where plant regeneration enhanced up to 2-5 folds. 30 They suggested that partial desiccation can be useful in stimulating regeneration response.…”

Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant

“…According to Lee and Huang [27] , the equilibrium of cytokinin and auxin is having a significant effect in the commencement of rejuvenation of stimulated callus [27] and are believed to impact plant cell cycle and ability of morphogenesis [21] . Apart from the established role of BAP in inducing shoot multiplication [ 1 , 20 , 47 ] many workers have reported the shoot induction on auxin and cytokinin combination also [ 4 , 12 , 38 ]. As in present study highest regeneration frequency has been obtained on BAP (5.0 mg/L) and NAA (0.5 mg/L) combination, earlier some workers have also reported shoot induction on same combination [ 33 , 45 ].…”

“…This combination of technologies can lead to creation of novel rice types having superior quality and high crop produce as well as stress tolerance to both abiotic and biotic stress [ 19 , 42 ]. For the efficient application of biotechnology in enhancement of rice yield, successful in vitro plant regeneration is critical [4] . The in vitro growth process is a crucial step in genetic transformation and provides substrate options that might be receivers of foreign DNA [16] .…”