In vitro regeneration ofExacum trinervium

Tennakoon, Samawansha; Peiris, S. E.; Eeswara, J. P.

doi:10.17660/actahortic.2015.1104.36

Cited by 2 publications

(4 citation statements)

References 10 publications

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“…The single nodal cuttings (0.5 cm) from aseptic plantlets were established on MS medium (Murashige and Skoog, 1962) (pH-5.8) at 2mgL -1 BAP concentrations (Inokaet al, 2014;Tennakoon et al, 2015) with different colchicine concentrations (0, 10, 20, 30, 40and 50mgL -1 ). They were maintained in above medium for different durations (0, 7, 14 21and 28 days) and transferred to 10ml of MS basal solid medium containing 2 mgL -1 BAP (Dahanayake et al,2009).Cultures were incubated at 22±2°C at 16 h/ 8 h photoperiod under cool white fluorescent light with 1000-1300 Lux intensity.…”

Section: Colchicine Treatment and Effect Of Bap On Shoot Proliferatiomentioning

confidence: 99%

“…One month old shoots were selected from above experiments and introduced to MS medium containing 2mgL -1 IBA (Tennakoon et al, 2015). Number of roots per shoot, average root lengths and survival rate of plant were recorded at one month after transferring to the above medium.…”

Section: Root Induction From Shoots Of E Ritigalensismentioning

confidence: 99%

“…Plantlets were acclimatized on sterilized coir dust and sand medium (1:1 ratio) in culture jars for two weeks, in the culture room (16 h light 1000 lux and at 25±1°C) prior to introduce in to greenhouse. After that plants were transferred into red yellow podzolic soil and maintain in green house condition (Tennakoon et al, 2015).…”

Section: Acclimatization and Transferring Of Plantsmentioning

confidence: 99%

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In vitro induction of tetraploids from Exacum ritigalensis (Gentianaceae)

Perera

Dahanayake

Senanayake

et al. 2016

Trop Agric Res & Ext

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Exacum ritigalensis (Gentianaceae) (Sinhalese: Binara or Ginihiriya) has a potential to develop as a potted plant by improving the size of the flowers and inflorescence. The aim of the study was to establish an efficient and effective system for the production of tetraploid plants through colchicine treatment. Single nodal cuttings (0.5 cm) from aseptic plantlets were established on Murashige and Skoog basal medium (MS) supplemented with 2 mgL -1 BAP and different colchicine concentrations (0, 10, 20, 30, 40 and 50 mg -1 ). They were kept in different durations (0, 7, 14 21 and 28 days) and transferred into 10ml of MS basal medium containing 2 mgL -1 BAP. After one month, they were transferred to MS medium containing 2mgL -1 Indole-3-butyric acid (IBA) for rooting. Then root tips were collected from treated plants to observe chromosome number. Afterwards the selected diploid and tetraploid plants were acclimatized and transferred to field to observe the morphological characters. Factorial Complete Randomized Design (FCRD) with 10 replicates was used for each treatment and statistical analysis was carried out using Duncan's Multiple Range Test of SAS program (9.1.3).The highest percentage of tetraploid plantlets (26.6%) was observed from nodal cuttings treated with MS basal medium with 2mgL -1 BAP + 30mgL -1 colchicine for seven days. The chromosome number of diploid and tetraploid E. ritigalensis determined as 28 and 56 respectively. Cells of the tetraploid plants were comparatively larger cells than diploids plants. The stomata size of the tetraploid (2.96*2.27 μm 2 ) were larger than those of the diploids (1.94*1.48μm 2 ). Leaf shape of diploids was lanceolate while it was ovate or narrowly elliptic in tetraploids. Flower petals of diploid plants were overlapping along their entire length while in tetraploids flower petals were not overlapping. The plants height was significantly higher in diploid (67.00±9 cm) than in tetraploid (38.00±4 cm). Newly produced polyploids of E. ritigalensis have a potential to popularize as a potted plant in the floriculture trade.

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Section: Colchicine Treatment and Effect Of Bap On Shoot Proliferatiomentioning

confidence: 99%

Section: Root Induction From Shoots Of E Ritigalensismentioning

confidence: 99%

Section: Acclimatization and Transferring Of Plantsmentioning

confidence: 99%

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In vitro induction of tetraploids from Exacum ritigalensis (Gentianaceae)

Perera

Dahanayake

Senanayake

et al. 2016

Trop Agric Res & Ext

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“…The approach currently being used to increase their population is by seed germination. However, it is difficult to reproduce these plants due to the high rate of seed dormancy [7,9,10].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Efficient Micropropagation Protocol for Developing Plantlets of Exacum Bicolor Roxb. – An Endangered, Ornamental, and Antidiabetic Herb

Achar¹,

Vinjamuri²

2021

Asian J Pharm Clin Res

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Objective: Exacum bicolor Roxb. is an endangered medicinal herb due to overexploitation by humans and its inefficient vegetative reproduction. Here, we report an efficient and simple procedure for the regeneration of E. bicolor Roxb. using leaf as an explant. Methods: The optimal concentrations of the hormones needed for callus induction were determined by full factorial method using DOE (Design expert ver. 8.0). The hormones selected based on literature were kinetin, indole acetic acid, and 6-Benzylaminopurine (BAP). Multiple shoot regeneration was carried out in liquid and solid media with the optimal concentrations of the hormones obtained by DOE. Rooting was initiated using Murashige and Skoog media containing naphthalene acetic acid 0.5 mg/l, indole butyric acid (IBA) 1.0 mg/l, and gibberellic acid 3 0.5 mg/l along with 0.2% of activated charcoal. Results: Analysis of full factorial design run showed that BAP in combination with kinetin was effective for the growth of callus and multiple shoot regeneration was higher in liquid media (81.25%). The rate of rooting was observed to be 88.23% and the average number of roots was 0.26. Plantlets with budding apical region and well-established leaves and roots were observed in 30 days. Conclusion: The protocol reported here can be used for effective production of E. bicolor plants in a shorter duration compared to the conventional approach.

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In vitro regeneration ofExacum trinervium

Cited by 2 publications

References 10 publications

<i>In vitro</i> induction of tetraploids from <i>Exacum ritigalensis</i> (Gentianaceae)

<i>In vitro</i> induction of tetraploids from <i>Exacum ritigalensis</i> (Gentianaceae)

A Simple and Efficient Micropropagation Protocol for Developing Plantlets of Exacum Bicolor Roxb. – An Endangered, Ornamental, and Antidiabetic Herb

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