In vitro regeneration of mulberry plants from seedling explants of Morus indica cv. G4 through direct organogenesis

Sarkar, Tanmoy; Ravindra, K. N.; Doss, S. G.; Kumar, P.; Tewary, Pankaj

doi:10.1007/s00468-021-02186-9

Cited by 8 publications

(2 citation statements)

References 38 publications

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“…Similar result was observed in Quercus rubra L. from immature cotyledon-derived embryogenic callus which required nearly the same concentration of BA and GA3 for simultaneous development of both shoot and root at approximately 61 % [35]. In other plants, germination of somatic embryos from leaf and internode of Wedelia calendulacea Less [31] and Podophyllum peltatum L [32] required GA3 at concentration of 0.5 and 1.0 mg/L, 2 and 4 times, respectively, higher than that used in the present study (0.25 mg/L). In genetically transformed avocado SEs, germination needed a very high concentration of GA3 at 10 mg/L [34].…”

Section: Effects Of Types Of Explants and Ga3 On Development Of Sesupporting

confidence: 77%

“…Plant regeneration obtained from single flower and petal was 37.5 and 12.5 %, respectively (Table 2). GA3 has been reported to facilitate germination of somatic embryos in in vitro conditions of many plant species [31][32][33]. However, the response to its concentration was quite different depended on initial cultured explant and plant species as well.…”

Section: Effects Of Types Of Explants and Ga3 On Development Of Sementioning

confidence: 99%

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Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Tongtape,

Te-chato,

Yenchon

2023

Trends Sci

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Rubber tree is economically important rubber producing plant of Thailand. At present, a rubber tree plantation is susceptible to white root disease. Therefore, the use of rootstock from early introduce clone that proved to be resistant to white root disease could help sustain growing of rubber tree. Thus, the objectives of this research were to study the effects of plant growth regulators and different explants on somatic embryo (SE) induction of this rubber clone and assessment genetic stability. The results revealed that mix flower explant cultured on MS medium supplemented with 2.0 mg/L 6-benzyladenine (BA) and 1.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) provided callus subsequent somatic embryo (SE) formation at the highest frequency of 39.84 % and number of cotyledonary embryos (CEs) at 3.25 embryos /callus after 3 passages of subculture in the same culture medium (4 weeks/passage). SEs germinated into embryonic axis at 50 % and further development into shoot at 25 % after transfer to 0.25 mg/L GA3 containing MS medium with the best concentrations of BA and 2,4-D for 4 weeks. The assessment gene stability by RAPD and SSR markers showed no variation between mother plant and in vitro plantlets. In this work, a novel explant source - the floral section of the rubber tree - was used for the first time in order to design an effective technique for in vitro somatic embryogenesis. HIGHLIGHTS The first report on plantlets regeneration using floral explant of rubber tree This mother plant is proved to be resistance to Phytopthora leaf fall and white root diseases Assessment genetic stability of regenerated plantlets by SSR and RAPD markers GRAPHICAL ABSTRACT

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Section: Effects Of Types Of Explants and Ga3 On Development Of Sesupporting

confidence: 77%

Section: Effects Of Types Of Explants and Ga3 On Development Of Sementioning

confidence: 99%

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Tongtape,

Te-chato,

Yenchon

2023

Trends Sci

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Transgenic Mulberry (Morus Spp.) for Stress Tolerance: Current Status and Challenges

Sarkar

Raghunath

Sivaprasad³

et al. 2023

Compendium of Plant Genomes

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Selection of F1 mulberry (Morus indica) genotypes for optimal and sub-optimal input conditions based on leaf yield, quality, and yield attributes

Sarkar,

Gowda,

Doss

et al. 2023

Trees

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In vitro regeneration of mulberry plants from seedling explants of Morus indica cv. G4 through direct organogenesis

Cited by 8 publications

References 38 publications

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Somatic Embryo (SE) Formation from Culturing Floral Explants of Rubber Tree (Hevea brasiliensis Muell. Arg.) and Assessment of Genetic Stability by RAPD and SSR Markers

Transgenic Mulberry (Morus Spp.) for Stress Tolerance: Current Status and Challenges

Selection of F1 mulberry (Morus indica) genotypes for optimal and sub-optimal input conditions based on leaf yield, quality, and yield attributes

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