In vitro regeneration of Pistacia vera L. from nodal explants

Abstract

To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg•l -1 . For axillary shoot proliferation, the excellent result was obtained using 2.0 mg•l -1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon ® ). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%.

Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

“…Plant materials were collected from Aq Beraz, Hurand, Ahar County, East Azarbaijan province, Iran (39°00′39″N 47°23′34″E) in the early growing season (May 2018) of R. coriaria . The current year stem segments with axillary and lateral buds (2–3 cm length), were exposed to sterilization procedures 23 , 28 . The explants (2–3 cm segments with lateral buds) were washed thoroughly under running tap water for 25 min and then treated with few drops of tween-20 and 1 g/L of mancozeb (antifungal) for one minute with constant shaking by hand, followed by three successive washings with distilled water.…”

“…The basal MS 29 medium supplemented with 20 mg/L casein hydrolysate 30 (Duchefa, Germany), 100 mg/L phloroglucinol 31 , 0.2% activated charcoal 23 , 32 (used to reduce tissue oxidation due to phenolic compounds during explant establishment), 3% (w/v) sucrose and 7 g/L agar was used in this study. Different PGRs including 6-benzylamino purine (BAP) as cytokinin and indol-3-butyric acid (IBA) and naphthalene acetic acid (NAA) as auxin (Duchefa, Germany) are used at different concentrations (mg/L).…”

“…Despite, its phytochemistry, pharmacological properties and industrial applications, few studies have been performed on the micropropagation of Sumac 16,17 . Several micropropagation procedures have been published for different species of the Anacardiaceae family such as Pistacia vera [18][19][20][21][22][23] . However, a general protocol has not been reported for efficient in vitro regeneration of this important species.…”

Advances in Plant Breeding Strategies: Nut and Beverage Crops

“…Pistacia vera and UCB-1 hybrid pistachio through shoot tips from juvenile 1-3-year-old seedling culture on modified Driver-Kuniyuki walnut medium (Parfitt and Almehdi 1994); Pistacia rootstocks such as P. khinjuk, P. atlantica, P. terebinthus and P. integerrima via meristem and shoot culture on MS medium containing 4 mg l −1 6-Benzylaminopurine (BAP) (Çetiner et al 1996); P. atlantica rootstock using apical or axillary bud explants culture on a modified Quoirin and Lepoivre (1977) supplemented with BAP plus naphthaleneacetic acid (NAA) (Mederos et al 1997); khinjuk pistachio (P. khinjuk) through seedling apical shoot-tip culture on MS medium with Gamborg vitamins 1 mg l −1 N6-benzyladenine (BA) (Tilkat et al 2005); pistachio (P. vera cv. Kirmizi) through nodal explants culture on MS medium containing different cytokinins and silver nitrate (AgNO 3 ) (Ozden-Tokatli et al 2005); P. lentiscus from axenic seedling-derived explants culture on MS medium supplemented 1 mg l −1 BA (Yıldırım 2012); pistachio (P. vera) nodal stem segments excised from 30-day-old aseptic seedling culture on MS containing Gamborg (B5) vitamins and supplemented with 4 mg l −1 (BA) (Benmahioul et al 2012a, b); P. vera Siirt, P. atlantica and P. khinjuk via shoot tips and nodal buds culture on MS medium containing 4 mg L −1 BA and 0.1 mg L −1 GA3 in a temporary immersion bioreactor system (RITA) (Akdemir et al 2014); genotypes of lentisk, P. lentiscus, through shoot tip explants (Kilinç et al 2015) and P. vera via nodal explants on MS medium supplemented different cytokinins and various concentrations (Benmahioul et al 2016). Also, a few studies have used adult material as an explant in micropropagation of pistachio such as P. vera cultivars: Mateur on MS medium (Abousalim andMantell 1992, Dolcet-Sanjuan andClaveria 1995); Antep via nodal segments on MS medium (Onay 2000a, b); Siirt (Onay et al 2004); male pistachio (P. vera) cv.…”

Establishment of optimized in vitro disinfection protocol of Pistacia vera L. explants mediated a computational approach: multilayer perceptron–multi−objective genetic algorithm

“…Various attempts have been reported regarding the adoption of in vitro culture technology that revealed, most often, various physiological disorders (browning, shoot apical necrosis, phenolic compounds exudation, vitrification …) [ 35 , 36 ]. To those, the hurdle of the culture aseptic initiation was frequently reported which is due to the surface colonizers as well as endogenous contamination [ 37 – 39 ]. Consequently, this species is still underexploited in arid and semi–arid regions.…”