In Vitro Regeneration of Pongamia pinnata Pierre

Sujatha, K.; Hazra, Sulekha

doi:10.5010/jpb.2006.33.4.263

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Shoots rooted and hardened simultaneously to obtain hardened plant in one step effects on the growth of S. anacardium shoots in bottles. A similar observation was noted in cultures of Pongamia pinnata(Sujatha and Hazra 2006). Influence of culture vessel on cultures of woody species has been studied extensively(McClelland and Smith 1990;Joshi et al 2009).…”

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confidence: 53%

In vitro regeneration of Semecarpus anacardium L. from axenic seedling-derived nodal explants

Panda

Hazra

2010

Trees

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Semecarpus anacardium (Anacardiaceae), a deciduous forest tree, is a potent source of medicinal compounds. Poor seed viability of this species limits the conventional propagation practice. Proliferation of shoots from axillary meristem was achieved in semisolid WPM medium supplemented with BAP 4.44 lM and KN 4.64 lM. Factors including culture vessels, gelling agents and antioxidants were identified and optimized for proliferation and growth of shoots in vitro. Cotton-plugged culture vessels were more favorable. Phytagel 0.2% as gelling agent and activated charcoal 0.2% as antioxidant were superior to other agents and antioxidants tested. All the shoots rooted in half-strength WPM liquid medium with IBA 2.46 lM. Rooted shoots survived (91%) in the soil-sand 1:1 mixture. Ex vitro rooting of shoots and hardening of plants were achieved in 80% of the explants in the soil-sand mixture. Hardened plants were maintained in a greenhouse. This is the first report on in vitro regeneration of Semecarpus anacardium.

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confidence: 53%

In vitro regeneration of Semecarpus anacardium L. from axenic seedling-derived nodal explants

Panda

Hazra

2010

Trees

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“…The pods were washed thoroughly in running tap water and were surface sterilized following the procedure described for mature seeds (Sujatha and Hazra 2006). Aqueous solution of mercuric chloride (0.1%) was used as a sterilant.…”

Section: Methodsmentioning

confidence: 99%

De novo organogenesis and plant regeneration in Pongamia pinnata, oil producing tree legume

Sujatha

Panda

Hazra

2008

Trees

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Role of Thidiazuron (TDZ) in inducing adventitious organogenesis in Pongamia was studied. TDZ at different concentrations (0, 0.45, 2.27, 4.54, 6.71, 9.08, 11.35, 13.12 and 22.71 lM) were used for induction of caulogenic bud formation in deembryonated cotyledon explants. Each cotyledon was cut into three segments and identified as proximal, middle and distal. Duration of TDZ exposure, influence of the segment and orientation of the explant were studied. TDZ at 11.35 lM concentration was optimum for the induction of shoots and rapid elongation. Shoots induced at higher concentration elongated after several passages in growth regulator free medium, thereby extending the period of differentiation. Exposure of the explant for 20 days yielded more number of buds than 10 days. Proximal segment of the cotyledon was more responsive. Contact of abaxial surface in the medium was more effective and generated more buds than the adaxial side. Buds differentiated and elongated on transfer to MS basal medium for 8-12 passages of 15 days each. Rooting and elongation of shoots was achieved in charcoal supplemented half-strength MS medium. Rooted plantlets survived on transfer to sand soil mixture. The plants were hardened and transferred to green house. This is the first report on in vitro regeneration of Pongamia pinnata via adventitious organogenesis using TDZ. This protocol may find application in studies in genetic transformation, isolation of somaclonal variants and in induction of mutants. It also provides a system to study the inhibitory role of TDZ on shoot differentiation.

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“…De-embryonated cotyledons were used as explants for regeneration studies (Sujatha et al, 2008). Incorporation of benzyl amino purine (BAP) on medium induces shoots proliferation from the cotyledonary nodes of seedling explants (Sujatha and Hazra, 2006). Sugla et al (2007) reported the complete protocol for multiplication of P. pinnata using cotyledonary nodes derived from axenic seedlings.…”

Section: Tissue Culturementioning

confidence: 99%