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Oil content, fatty acid composition and karanjin content were studied in developing pongamia seeds, at intervals of 3 weeks from 30 weeks after flowering up to 42 weeks. Three marked stages in seed development were observed at the early green pod stage, the middle half brown stage and the late dark brown stage. Significant variation in seed biomass, pod and seed characteristics were observed. A significant (P \ 0.01) decrease in the moisture content of the seeds was observed during seed development. The oil content gradually increased from 32.06 to 36.53 % as the seed matured. A significant variation in fatty acid composition was detected across all stages of seed development. Palmitic acid (16:0) content marginally decreased from 11.81 to 10.18 %, while stearic acid (18:0) and linolenic acid (18:3) remained constant at all stages of seed maturity. A steady increase in oleic acid (18:1) content from 38.11 to 49.11 % was observed, while the linoleic acid (18:2) content decreased from 30.14 to 18.85 %. The iodine value increased, while the saponification number of oil decreased during seed development. The increase in karanjin content was steady. Seeds harvested after 42 week after flowering yielded the maximum oil with high oleic acid content which could be suitable for biodiesel production. KeywordsFatty acid profile Á Karanjin Á Oil content Á Pongamia pinnata Á Seed development Abbreviations WAF Weeks after flowering FAME Fatty acid methyl esters DMRT Duncan multiple range test MC

Efficacy of two dominant molecular markers, namely, amplified fragment length polymorphism (AFLP) and three endonuclease (TE)-AFLP, were assessed in 20 individuals of the biodiesel species Pongamia pinnata. Four primer combinations generated a total of 254 and 194 bands in AFLP and TE-AFLP, respectively. Both techniques could unequivocally identify each accession used in this study. The Jaccard's similarity coefficient ranged from 0.30 to 0.90 for AFLP and from 0.25 to 0.85 for TE-AFLP. The correlation coefficient between AFLP and TE-AFLP dendrogram was 0.56 which was low but significant (P< 0.001). Values of effective multiplex ratio, marker index, and resolving power were markedly higher in AFLP than in TE-AFLP. However, the band intensities across different lanes were uniform in TE-AFLP leading to easy and accurate scoring of gels which resulted in slightly higher bootstrap values with TE-AFLP data as compared to AFLP data. Inferences based on TE-AFLP data had similar level of biological relevance as compared to AFLP data when location and diameter of trees were taken in to consideration. However, the easy scorability of TE-AFLP profiles is extremely important and especially desirable in studies requiring genotyping of large number of individuals distributed across many gels.

3-Alkylidene-1,3-dihydroisobenzofurans exhibited moderate antidepressant activity as evaluated by forced swim and tail suspension test methods. Virtual screening was carried out by docking the designed compounds into the serotonin binding sites of arabinase protein to predict the analogue binding mode of the compounds to the SSRIs.

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