In vitro Regulation of the Lipolysis of Adipose Tissue

Davies, J. Islwyn

doi:10.1038/218349a0

Cited by 46 publications

(11 citation statements)

References 23 publications

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“…The antilipolytic effect of adenosine is well documented [19,20,[22][23][24][25][26][27][28][29][30]321. The facilitation of the antilipolytic action of insulin appears to be an extension of this mechanism since it can be observed at concentrations of adenosine which affect lipolysis only marginally, but cause a pronounced fall of cyclic AMP levels.…”

Section: Discussionmentioning

confidence: 99%

Facilitation by Adenosine of the Action of Insulin on the Accumulation of Adenosine 3′:5′‐Monophosphate, Lipolysis, and Glucose Oxidation in Isolated Fat Cells

Schwabe

Schönhöfer

Ebert

1974

European Journal of Biochemistry

145

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The modulation of insulin action by adenosine has been studied in lipid and glucose metabolism of isolated rat fat cells. When 20000 cells per ml were incubated, insulin failed to antagonize the effect of 1 pM noradrenaline on adenosine 3': 5'-monophosphate (cyclic AMP) levels and lipolysis. At high concentrations (100000 cells per ml) both parameters were strongly depressed by insulin. The addition of 0.1 pM adenosine to dilute cell suspensions decreased cyclic AMP accumulation in response to noradrenaline, but only marginally reduced lipolysis, thus producing the same situation as seen in concentrated cell suspensions. Furthermore, insulin antagonized the cyclic AMP accumulation and lipolysis caused by adenosine deaminase (1 pg per ml) or theophylline (0.3 mM). These two agents only moderately elevated cyclic AMP levels similar to the effect of noradrenaline in the presence of adenosine. These data strongly indicate that insulin is able to counteract hormone effects on cyclic AMP formation and lipolysis only at low cellular levels of the cyclic nucleotide.Stimulation of glucose oxidation by insulin in the range between 10 and 100 pU per ml was markedly enhanced in the presence of 0.1 pM adenosine when only 5000 fat cells per ml were incubated. On the other hand, adenosine deaminase reduced the action of insulin in the range between 1 and 300 pU per ml in concentrated cell suspensions (100000 cells per ml). The uptake of adenosine and the ATP levels in fat cells were not altered by insulin, except at high concentrations of adenosine (0.1 mM). Theophylline (0.5 mM) and dipyridamole (20 pM) abolished the stimulatory effect of both insulin and adenosine on glucose oxidation. Increasing concentrations of adenosine were able to overcome the inhibitory effect of theophylline but not that of dipyridamole. These data suggest that adenosine may have an essential regulatory role which can be described as facilitation of insulin actions, even though different mechanisms apply to lipolysis and glucose oxidation.

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Section: Discussionmentioning

confidence: 99%

Facilitation by Adenosine of the Action of Insulin on the Accumulation of Adenosine 3′:5′‐Monophosphate, Lipolysis, and Glucose Oxidation in Isolated Fat Cells

Schwabe

Schönhöfer

Ebert

1974

European Journal of Biochemistry

145

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“…The inhibitory effect of caffeine on phosphodiesterase activity is well correlated with its effect on lipolysis in the intact fat-cell (Brodie et al 1966). This correlation could be extended in some but not all instances to the selection of nucleosides and free bases tested (Davies, 1968).…”

mentioning

confidence: 59%

Significance of the caffeine-like effect of various purines, pyrimidines and derivatives on adipose-tissue phosphodiesterase

Peers

Davies

1971

Biochemical Journal

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“…There have been many reports concerning the effects of nucleotides and/or nucleosides on various phosphodiesterase preparations from beef heart (soluble type) (Goren and Rosen 1971;Fain et al 1972), adipose tissues (soluble type) (Gylyassy 1971), membrane bound type (Peers and Davies 1971;Fain et al 1972), mixture type (Davies 1968), liver (soluble type) (Beavo et al 1971) and brain (mixture type) (Roberts and Simonsen 1970;Harris et al 1973) and the results indicate that nucleosides have inhibiting effects in general, whereas nucleotides have various effects. In those studies, the effects of nucleotides and/or nucleosides on phosphodiesterase were examined at a single dose.…”

Section: Discussionmentioning

confidence: 99%

Effects of nucleotides and nucleosides on the activity of cyclic AMP phosphodiesterase from rat brain.

Murakami

Miura

Ito

et al. 1983

Tohoku J. Exp. Med.

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In vitro Regulation of the Lipolysis of Adipose Tissue

Cited by 46 publications

References 23 publications

Facilitation by Adenosine of the Action of Insulin on the Accumulation of Adenosine 3′:5′‐Monophosphate, Lipolysis, and Glucose Oxidation in Isolated Fat Cells

Facilitation by Adenosine of the Action of Insulin on the Accumulation of Adenosine 3′:5′‐Monophosphate, Lipolysis, and Glucose Oxidation in Isolated Fat Cells

Significance of the caffeine-like effect of various purines, pyrimidines and derivatives on adipose-tissue phosphodiesterase

Effects of nucleotides and nucleosides on the activity of cyclic AMP phosphodiesterase from rat brain.

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