In vitro release of dimethyloxaloylglycine and l-mimosine from bovine bone mineral

Dimethyloxalylglycine lyophilized onto bone substitutes increase vessel area in rat calvarial defects

“…However, the selection was based on various in vitro assays; thus, we had a rational for this decision (Agis et al. ; Vinzenz et al. ).…”

“…In vitro , DMOG and DFO are released within at least 48 h, based on a bioassay on osteoclastogenesis, VEGF expression, and cytotoxicity (Agis et al. ; Vinzenz et al. ).…”

Bone substitute materials supplemented with prolyl hydroxylase inhibitors decrease osteoclastogenesis in vitro

“…12 In brief, 100 mg of bone substitute material was soaked with 300 mL of the 3 mM prolyl hydroxylase inhibitor solutions at room temperature and frozen. The bovine bone mineral preparations were then lyophilized using an ALPHA 1-2 LDplus freeze dryer (Martin Christ, Osterode am Harz, Germany).…”

“…[5][6][7][8] Strategies have therefore been developed where bone substitute materials release prolyl hydroxylase inhibitors to enhance angiogenesis and bone regeneration. [9][10][11][12] These "hypoxia mimetic agents" either inhibit the oxygenases directly or complex Fe 21 , thereby preventing hypoxia-inducible factor (HIF)21alpha degradation. The most commonly applied prolyl hydroxylase inhibitors are dimethyloxalylglycine (DMOG), desferrioxamine (DFO), and L-mimosine (L-MIM).…”

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

“…We evaluated if loading with washed and unwashed PSEC changes the morphology and cell attachment on the membrane. An established bioassay was applied to assess the mitogenic activity released from the CBM over time [27]. In addition, we assessed the release kinetics directly based on total protein, PDGF-BB, and TGFβ1.…”