In Vitro Response Growth of Stevia Rebaudiana Under Salinity and Drought

Mubarak, Mohamed; Belal, A. H.; ElSarag, Eman; NasrElDeen, Taimor

doi:10.21608/sinjas.2012.78374

Cited by 5 publications

(5 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most probable reason for growth reduction in the case of root formation is the change in metabolic activities resulting in reduction in cell division, elongation, and differentiation of roots. Mubarak et al (2012) obtained significantly less growth after exposure to rising salt stress. The control treatment produced the best results with respect to shoot number, shoot length (cm), node number, leaf number, root number, root length (cm), and % survival rate.…”

Section: Discussionmentioning

confidence: 91%

“…Their studies reported changes in growth, chlorophyll content, proline accumulation, and protein and sugar contents. Moreover, the influence of salt and drought stress on S. rebaudiana growth dynamics was revealed by Mubarak et al (2012). A field study of NaCl stress in S. rebaudiana revealing effects on growth, photosynthetic pigments, diterpene glycosides, and ion content in roots and shoots was performed by Shahverdi et al (2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salt stress by NaCl alters the physiology and biochemistry of tissue culture-grownStevia rebaudiana Bertoni

Javed¹

2019

Turk J Agric For

View full text Add to dashboard Cite

This study reports the response to salinity stress (100 mM, 200 mM, and 300 mM NaCl concentration) exposure of the commercially valuable medicinal plant Stevia rebaudiana during micropropagation for 4 weeks. The significant enhancement of physiological parameters, steviol glycosides (SGs), i.e. rebaudioside A and stevioside, as examined by high-performance liquid chromatography, and nonenzymatic antioxidant activities, i.e. total phenolic content, total flavonoid content, total antioxidant capacity, total reducing power, and DPPH-free radical scavenging activity, was observed during the shoot formation process under up to 100 mM NaCl stress. Callus formation produced similar results regarding physiology and antioxidant assays, except that it produced merely a negligible amount of SGs. Contrarily, root formation showed marked susceptibility to 100 mM, 200 mM, and 300 mM NaCl concentrations and reduced growth parameters, sweetening compounds, and secondary metabolites. Hence, NaCl plays the role of abiotic stress elicitor, causing accumulation of reactive oxygen species and thus altering metabolic processes and physiology of Stevia under in vitro culture conditions.

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Salt stress by NaCl alters the physiology and biochemistry of tissue culture-grownStevia rebaudiana Bertoni

Javed¹

2019

Turk J Agric For

View full text Add to dashboard Cite

show abstract

“…Around 19.5% of irrigated agricultural land is considered to be saline [ 64 ]. Stevia plantlet growth decreases as salinity increases [ 65 , 66 , 67 ]. Our previous study also showed that stevia germination and growth is impacted by salt stress [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

The Content of Phenolic Compounds in Stevia rebaudiana (Bertoni) Plants Derived from Melatonin and NaCl Treated Seeds

Simlat

Ptak

Wójtowicz

et al. 2023

Plants

View full text Add to dashboard Cite

Stevia is a plant with many beneficial properties. It contains not only steviol glycosides, which are used as non-caloric natural sweeteners, but also a number of metabolites with antioxidant properties. This study examined the content of both phenolic acids and flavonoids in stevia leaves as an effect of treating seeds with melatonin and conducting germination in NaCl conditions. The results of our research indicated higher amounts of phenolic acids compared to flavonoids in stevia leaves. Among these acids, isochlorogenic, rosmarinic, and chlorogenic acids were accumulated in the largest amounts, regardless of the germination conditions. For 5 and 100 µM of melatonin treatments, the content of both phenolic acids and flavonoids increased. However, in salinity conditions (50 mM NaCl), 500 µM of melatonin had the most favorable effect on the synthesis of phenolic acids. The phenolic acids in that case reached a level three-times higher than that in the samples with the same melatonin concentration but without NaCl. We also found that the content of phenolic compounds varied depending on the age of the leaves. To the best of our knowledge, this is the first study to describe the effect of melatonin and NaCl on the synthesis on phenolic acids and flavonoids in stevia.

show abstract

“…The finding of this study leads to the conclusion that significantly less growth was observed after exposure to rising salt stress, while the control treatment produced the best results concerning shoot number, shoot length (cm), number of leaves, number of multiple shoots, leaf number, root number, root length (cm), % survival callus, and % regeneration. The most probable reason for growth reduction in the case of root formation is the change in metabolic activities resulting in a reduction in cell division, elongation, and differentiation of roots [ 32 ]. Plant height decreased under NaCl concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, tissue culture strategies have been utilized as a valuable instrument to clarify the mechanisms engaged with salt tolerance by utilizing chosen salt-lenient cell lines in vitro; consequently, producing salt-lenient plants [ 29 , 30 , 31 ]. Stevia plantlet growth characters decrease as salinity stress levels increase [ 32 ]. Zeng et al [ 33 ] reported that S. rebaudiana is mildly tolerant to salt stress.…”

Section: Introductionmentioning

confidence: 99%

Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Azzam

Al-Taweel

Abdel-Aziz³

et al. 2021

Plants

View full text Add to dashboard Cite

Stevia rebaudiana Bertoni is a little bush, which is cultivated on a large scale in many countries for medicinal purposes and used as a natural sweetener in food products. The present work aims to conduct a protocol for stevia propagation in vitro to produce and introduce Stevia rebaudiana plants as a new sweetener crop to Egyptian agriculture. To efficiently maximize its propagation, it is important to study the influence of stress factors on the growth and development of Stevia rebaudiana grown in vitro. Two stevia varieties were investigated (Sugar High A3 and Spanti) against salt stress. Leaves were used as the source of explants for callus initiation, regeneration, multiplication and rooting. Some stress-related traits, i.e., photosynthetic pigments, proline contents, and enzyme activity for peroxidase (POD), polyphenol oxidase (PPO), and malate dehydrogenase (MDH) were studied. Murashig and Skoog (MS) medium was supplemented with four NaCl concentrations: 500, 1000, 2000, and 3000 mgL−1, while a salt-free medium was used as the control. The data revealed that salinity negatively affected all studied characters: the number of surviving calli, regeneration%, shoot length, the number of multiple shoots, number of leaf plantlets−1, number of root plantlets−1, and root length. The data also revealed that Sugar High A3 is more tolerant than Spanti. The total chlorophyll content decreased gradually with increasing NaCl concentration. However, the opposite was true for proline content. Isozyme’s fractionation exhibited high levels of variability among the two varieties. Various biochemical parameters associated with salt tolerance were detected in POD. Namely, POD4, POD6, POD 9 at an Rf of 0.34, 0.57, and 0.91 in the Sugar High A3 variety under high salt concentration conditions, as well as POD 10 at an Rf of 0.98 in both varieties under high salt concentrations. In addition, the overexpression of POD 5 and POD 10 at Rf 0.52 and 0.83 was found in both varieties at high NaCl concentrations. Biochemical parameters associated with salt tolerance were detected in PPO (PPO1, PPO2 and PPO4 at an Rf of 0.38, 0.42 and 0.62 in the Sugar High A3 variety under high salt concentrations) and MDH (MDH 3 at an Rf of 0.40 in both varieties at high salt concentrations). Therefore, these could be considered as important biochemical markers associated with salt tolerance and could be applied in stevia breeding programs (marker-assisted selection). This investigation recommends stevia variety Sugar High A3 to be cultivated under salt conditions.

show abstract

In Vitro Response Growth of Stevia Rebaudiana Under Salinity and Drought

Cited by 5 publications

References 1 publication

Salt stress by NaCl alters the physiology and biochemistry of tissue culture-grownStevia rebaudiana Bertoni

Salt stress by NaCl alters the physiology and biochemistry of tissue culture-grownStevia rebaudiana Bertoni

The Content of Phenolic Compounds in Stevia rebaudiana (Bertoni) Plants Derived from Melatonin and NaCl Treated Seeds

Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Contact Info

Product

Resources

About