In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells

Takahashi, Jyunichiro; Toshima, Gen; Matsumoto, Yukie; Kimura, Fumiko; Kiuchi, Takanobu; Hamada, Kentaro; Hata, Keisuke

doi:10.1007/s11418-011-0542-x

Cited by 13 publications

(9 citation statements)

References 13 publications

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“…LipoCULTURE by Skylight Biotech Inc. A novel screening system for antidyslipidemic agents using cultured cells has been reported by Takahashi et al 75 .…”

Section: Principles and System Configuration Of The Liposearch Systemmentioning

confidence: 99%

Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using “Spherical Particle Model”

Okazaki

Yamashita

2016

J. Oleo Sci.

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“…LipoCULTURE by Skylight Biotech Inc. A novel screening system for antidyslipidemic agents using cultured cells has been reported by Takahashi et al 75 .…”

Section: Principles and System Configuration Of The Liposearch Systemmentioning

confidence: 99%

Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using “Spherical Particle Model”

Okazaki

Yamashita

2016

J. Oleo Sci.

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“…It was previously reported that ethanol and water extracts of Brasenia schreberi exhibit strong inhibitory activities against triglyceride and cholesterol secretions from human hepatoma cells [22], although the inhibitory compound has not yet been purified and identified. There is no such report for Petasites japonicus; as far as we know, Rosa species contain polyphenolic compounds with antioxidant activities [12].…”

Section: Rt Inhibition By Extracts Of Edible Flowersmentioning

confidence: 99%

Inhibition of the DNA polymerase and RNase H activities of HIV-1 reverse transcriptase and HIV-1 replication by Brasenia schreberi (Junsai) and Petasites japonicus (Fuki) components

et al. 2015

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Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) possesses two distinct enzymatic activities: those of RNA- and DNA-dependent DNA polymerases and RNase H. In the current HIV-1 therapy, all HIV-1 RT inhibitors inhibit the activity of DNA polymerase, but not that of RNase H. We previously reported that ethanol and water extracts of Brasenia schreberi (Junsai) inhibited the DNA polymerase activity of HIV-1 RT [Hisayoshi et al. (2014) J Biol Macromol 14:59-65]. In this study, we screened 43 edible plants and found that ethanol and water extracts of Brasenia schreberi and water extract of Petasites japonicus strongly inhibit not only the activity of DNA polymerase to incorporate dTTP into poly(rA)-p(dT)15 but also the activity of RNase H to hydrolyze the RNA strand of an RNA/DNA hybrid. In addition, these three extracts inhibit HIV-1 replication in human cells, with EC50 values of 1-2 µg/ml. These results suggest that Brasenia schreberi and Petasites japonicus contain substances that block HIV-1 replication by inhibiting the DNA polymerase activity and/or RNase H activity of HIV-1 RT.

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“…To assess a role of FAS in regulating liver steatosis by miR-130a-3p, we investigated the effects of miR-130a-3p on SO-induced steatosis (29) in HepG2 cells. Overexpression of miR-130a-3p significantly prevented SO-increased TG content in these cells compared with control cells (Fig.…”

Section: Overexpression Of Mir-130a-3p Reverses Liver Steatosis In Dbmentioning

confidence: 99%

A Novel Function of MicroRNA 130a-3p in Hepatic Insulin Sensitivity and Liver Steatosis

Xiao

Liu

et al. 2014

Diabetes

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MicroRNAs (miRNAs) are endogenous, noncoding, short, single-stranded RNAs that are evolutionarily conserved and believed to play a role in controlling a variety of biological processes. The roles of miRNAs in insulin resistance and liver steatosis, however, are largely unknown. The objective of this study was to evaluate the roles of miR-130a in the regulation of insulin sensitivity and liver steatosis. In our current study, we observed that overexpression of miR-130a-3p increases insulin signaling in both HepG2 cells and primary mouse hepatocytes, and silencing of miR-130a-3p has the opposite effects. However, miR-130a-5p has no effect in the regulation of insulin signaling. Consistently, whole-body and hepatic insulin sensitivity are improved in mice injected with adenoviruses that overexpress miR-130a-3p. Furthermore, we provided evidence showing that growth factor receptor–bound protein 10 is required for miR-130a-3p–regulated insulin sensitivity. On the other hand, we observed that expression of miR-130a-3p is decreased in the livers of db/db mice and that adenovirus-mediated overexpression of miR-130a-3p reverses insulin resistance and liver steatosis, the latter of which is achieved via suppressing fatty acid synthase expression in these mice. This study identifies a novel function for hepatic miR-130a-3p in the regulation of insulin sensitivity and liver steatosis.

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In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells

Cited by 13 publications

References 13 publications

Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using “Spherical Particle Model”

Recent Advances in Analytical Methods on Lipoprotein Subclasses: Calculation of Particle Numbers from Lipid Levels by Gel Permeation HPLC Using “Spherical Particle Model”

Inhibition of the DNA polymerase and RNase H activities of HIV-1 reverse transcriptase and HIV-1 replication by Brasenia schreberi (Junsai) and Petasites japonicus (Fuki) components

A Novel Function of MicroRNA 130a-3p in Hepatic Insulin Sensitivity and Liver Steatosis

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