In Vitro Screening of Antibacterial Properties of Rhus coriaria and Origanum vulgare against Some Pathogenic Bacteria

Abstract

Abstract-This study investigates the antibacterial property of Rhus coriaria (Sumac) and Origanum vulgare (Jatra) aqueous extracts against Escherichia coli ATCC 25922, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa ATCC 27835. Results confirm the resistance of the bacterial isolates against more than three antibiotics. The aqueous extract of R. coriaria showed the highest activity as an inhibitor against tested bacteria, while the aqueous extract of O. vulgare shows low effect against the above mentioned bacteria. MIC for R. coriaria and O. vulgare aqueous extracts were determined for four bacterial isolates. The MIC of O. vulgare against tested bacteria was >12%, while the MIC of R. coriaria was 4% for E. coli, <0.025% for both K. pneumoniae and P. mirabilis and 2% for P. aeruginosa. The phytochemical groups of both plants extract were analyzed; the results indicated both plants contain tannins, phenols and saponins, flavonoid, alkaloid, and phlobatanin. The antimitotic effect of both plants extracts were investigated on Allium cepa, and the extracts showed inhibitory role in the root growth in contrast to the control when grown in the tap water for 5 days. In addition, the 24 hours treatment of grown roots in tap water with both extracts resulted in significant decrease in mitotic index.Index Terms-Antibacterial agents, antimitotic index, E. coli, K. pneumoniae, plant extracts, P. aeruginosa, P. mirabilis.

Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies

“…vulgare [ 1 , 3 ]. In terms of chemical composition, research on O. vulgare species from various countries have previously been reported: Italy [ 4 ], Greek [ 5 , 6 ], Serbia [ 7 ], Egypt [ 8 ], Pakistan [ 9 , 10 ], China [ 10 ], India [ 11 ], Iraq [ 12 ], Macedonia [ 13 ]. Representatives of the following important classes of active principles were revealed: essential oil (with carvacrol and/or thymol, linalool, and p -cymene), polyphenols (flavonoids and phenolic acids), triperpenoids, and sterols [ 8 , 14 , 15 ].…”

“…Origanum vulgare ’s longstanding use in traditional medicine attracts even greater interest in designing new pharmaceutical formulations in various areas [ 2 ]. New investigations have shown many therapeutic properties: antimicrobial [ 12 ], antiviral [ 10 ], antioxidant [ 10 ], anti-inflammatory [ 15 ], antispasmodic [ 2 ], antiurolithic [ 9 ], antiproliferative [ 4 , 15 ], neuroprotective [ 16 ], etc. Because of its high antioxidant activity, O. vulgare is an important natural source for the preservation of different food or cosmetics [ 2 , 15 , 17 ].…”

Antibacterial and antibiofilm effect of sumac (Rhus coriaria L) fruits extracts against some multidrug_resistant pathogenic bacteria

“…While the phytochemical components of Sumac fruit alcoholic extract showed the presence of flavonoids, glycosides, phenols, terpenoids, steroids, saponins, resins, tannins, and a minute presence of alkaloids. With similar results, the phytochemical groups of Sumac extract in vitro were analyzed by (18), and the results indicated that the Sumac extract contains tannins, phenols, saponins, flavonoids, alkaloids, and phlorotannin. Rhus coriaria contains phenols and tannins, and as in many, the researchers explained the action of hydrophobic property of phenolic compounds in impairing the cellular function and membrane integrity as mentioned in (19), and also interpreted that the aqueous extracts of R. coriaria contain phenols, tannins, and others integrates and these may have an influence on the enzymatic system of bacteria especially those that prevent the plasmid replication or may effect on the cell membrane, especially on mesosome which is considered as the attachment point for plasmids.…”

Antimicrobial activity of some plant species used for the medical purpose in Turkey

“…Result from obtained study indicated that the extract of R. coriaria has antimicrobial activity as 20-33 mm zone of inhibition to the tested microorganisms and the MIC values of this extract were determined as 0.3125-2.5 mg/ml against the tested microorganisms (table 1-2). In addition, many reports demonstrated the extract of R. coriaria has the antibacterial activity against to B. subtilis, P. aeuroginosa and S. aureus [12,13] . R. coriaria, followed by P. terebinthus subsp.…”