In vitro screening of probiotics and synbiotics according to anti-inflammatory and anti-proliferative effects

Identification of One Novel Candidate Probiotic Lactobacillus plantarum Strain Active against Influenza Virus Infection in Mice by a Large-Scale Screening

“…Bacteria inducing higher levels of the anti-inflammatory cytokine IL-10 and lower levels of the proinflammatory cytokine IL-12 in vitro displayed the best protection in the murine colitis model (15). The human intestinal epithelial cell line HT-29 has been also used successfully to evaluate the immunomodulatory properties of bacteria (25,26). Once coincubation with bacterial strains was achieved, IL-8 quantification led to the selection of anti-inflammatory bacteria (reducing IL-8 production) and proinflammatory bacteria (enhancing IL-8 production) (25,27).…”

“…The human intestinal epithelial cell line HT-29 has been also used successfully to evaluate the immunomodulatory properties of bacteria (25,26). Once coincubation with bacterial strains was achieved, IL-8 quantification led to the selection of anti-inflammatory bacteria (reducing IL-8 production) and proinflammatory bacteria (enhancing IL-8 production) (25,27). After we tested our bacterial collection with these two cellular models, we selected 3 different responsive strains according to their immunomodulatory profile, one with a highly proinflammatory profile (L. plantarum CNRZ1997), one with a weakly proinflammatory profile (L. brevis VEL12208), and one with a markedly anti-inflammatory profile (L. paracasei VEL12195), for in vivo experiments using a model of A/PR8/34 strain infection in mice.…”

Exopolysaccharide-producing Bifidobacterium strains elicit different in vitro responses upon interaction with human cells

“…However, B. pseudocatenulatum A102 showed an opposite tendency in HT29 and Caco2. Thus, results obtained were highly dependent on the cell line tested which underlines the fact that the exclusive measurement of cellular line proliferation is not a valid method to determine the anti-carcinogenic potential of probiotic strains (Grimoud et al, 2010). Several authors have suggested that the immune system of an animal model was very much involved in preventing, or reducing, the tumour proliferation by bacterial EPS.…”

The Effects of Media on Pharmaceutically Relevant Transporters in the Human HT-29 Adenocarcinoma Cell Line: Does Culture Media Need to be Controlled?

“…Based on these previous observations, we examined the purported differentiation-dependent genotypic and phenotypic differences in the HT-29 cell line grown under the various conditions described above on the expression of 86 pharmaceutically relevant transporters and the transport of peptide-based substrates. Previous research with this cell line has primarily focused on single gene changes 31,32 and other characteristics such as cell-cycling, 33,34 proliferation, 35 inflammation, 36 and mucin secretion in response to a drug or some external stimuli. 37 However, to our knowledge, differentiation-dependent differences, specifically in a comprehensive transporter expression profile that occur due to media changes have not been previously demonstrated.…”