In vitro selection and characterization of DNA aptamers recognizing chloramphenicol

Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

“…25% ammonia (NH 3 $H 2 O), 25% glutaraldehyde (OHC(CH 2 ) 3 CHO), tetraethyl orthosilicate (TEOS), 1,6-hexanediamine, anhydrous sodium acetate, acetone, FeCl 3 $6H 2 O, NaF, NaOH, glycol, cyclohexane, ethanol were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). CAP aptamer (reported by Mehta et al (2011)) and its part complementary strand were synthesized by Shanghai Sangon Biological Science & Technology Company (Shanghai, China). The sequence of the CAP aptamer is 5 0 -biotin-AGCAGCA-CAGAGGTCAGATGACTTCAGTGAG TTGTCCCACGGTCGGC-GAGTCGGTGGTAGCCTATGCGTGCTACCGTGAA-3 0 , the sequence of its part complementary DNA is 5 0 eNH 2 eCGACCGTGGGACAACTC A-3 0 .…”

Selection of DNA aptamers that bind to four organophosphorus pesticides

“…Aptamers are superior to antibodies because of their broader range of application. They are able to bind not only to proteins (Tang et al 2007;Hasegawa et al 2008;Tsukakoshi et al 2010) but also to other molecules, including metal ions (Kawakami et al 2000), and small molecules (Mann et al 2005;Niazi et al 2008;Cruz-Aguado and Penner 2008;Kim et al 2010;Mehta et al 2011).…”

A “signal-on’’ aptasensor for simultaneous detection of chloramphenicol and polychlorinated biphenyls using multi-metal ions encoded nanospherical brushes as tracers

“…3, once the CAP and PCB72 were added to the biosensor, a competitive-type displacement reaction was happened between the targets (CAP and PCB72) and the tracers, resulting in peak current increased due to the release of tracers. The binding constant of CAP-aptamer and PCB72-aptamer coated magnetic beads are 1.31 Â 10 6 M À 1 and 1.67 Â 10 7 M À 1 , respectively (Mehta et al, 2011. With the increased concentration of CAP and PCB72, the current of lead and cadmium increased in the meantime, respectively.…”