In vitro-Selection and Regeneration of Hydroxyproline-Resistant Lines of Winter Wheat with Increased Proline Content and Increased Frost Tolerance

Dörffling, Karl; Dörffling, Helga; Lesselich, G.

doi:10.1016/s0176-1617(11)80968-5

Cited by 51 publications

(20 citation statements)

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“…Breeding for water stress tolerance by traditional methods is a time consuming and inefficient procedure [16]. Saadalla [17] reported plant breeders should have an effective and reliable screening method; relatively simple, accurate, inexpensive and well correlated with crop performance under actual stressful conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Ferede¹,

Mekbib²,

Assefa³

et al. 2018

Adv Crop Sci Tech

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Breeding for moisture stress tolerance by conventional methods is not satisfactory in view of current demand to increase crop productivity. Inducing somaclonal variation is a potential technique to obtain stress tolerant variants. The present study was conducted to evaluate the performance of tef somaclones (R1 generation) and their respective parental genotypes for drought tolerance. The materials used in this experiment were obtained from the R0 generation of previous in vitro culture experiment. Twelve seed derived somaclones; Melko 0, Melko 0.5, Melko 1, Melko 1.5, Gemechis 0, Gemechis 0.5, Gemechis 1, Gemechis 1.5, Pop12S20, Pop12S20.5, Pop12S21 and Pop12S21.5 and their respective parental genotypes (Melko, Gemechis and Pop12S2) were used under two set of experiments (i.e., under moisture stress and non-stress conditions) in pots under green house. The experiments were laid out in completely randomized design with three replications. Analysis of variance revealed that somaclones (R1 generation) and their parental genotypes showed highly significant (P ≤ 0.01) difference in all the measured traits under both moisture regimes. Comparisons were made between the somaclones and their parents under moisture stress and non-stress conditions for the measured traits. Under moisture stress condition superiority of the somaclones was observed except for number of spikelet/panicle and total number of tiller/plant. On the other hand, under non-stress condition better performance was observed from parental genotypes for number of spikelet/ panicle, panicle weight, panicle seed weight, plant weight, plant seed weight and grain yield. The results showed that all the somaclones and their parental genotypes were negatively affected by moisture stress for all studied parameters. However, somaclones were less affected by moisture stress as compared to the parental genotypes. Hence, in vitro culture induced somaclonal variation can effectively be used for selection and improvement of drought tolerant tef genotypes.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Ferede¹,

Mekbib²,

Assefa³

et al. 2018

Adv Crop Sci Tech

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“…Moreover, in several unicellular species, the intracellular concentration of proline serves as a defense against osmotic challenge (Csonka, 1989;Csonka and Hanson, 1991;Strom et al, 1983;Yancey, 1994). Also as it has been reviewed extensively elsewhere, the accumulation of proline is a common metabolic response of higher plants to water deficits, salinity stress and cold stress (Csonka and Baich, 1983;Dorffling et al, 1993;Hanson and Hitz, 1982;Stewart, 1981;Stewart and Larher, 1980;Thomson, 1980). In this article we review recent work on the metabolic functions of proline and P5C unravaled by the results of molecular studies from their deficiencies in humans.…”

Section: Introductionmentioning

confidence: 99%

The proline biosynthesis in living organisms

Aral

Kamoun

1997

Amino Acids

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Summary.In this article we review recent work on the physiology of proline and Al-pyrroline-5-carboxylate (P5C) in living organisms and consider recent progress in our understanding of the role of P5C synthetase in collagen metabolism and the regulation of urea cycle in vertebrates. Much of this recent progress has been made possible by advances in our knowledge of the enzymes and genes involved in proline biosynthesis in man. The availability of well characterized P5C synthetase deficiency in man has been an impetus for the cloning of the cDNA encoding for this enzyme from man and facilitated the establishment of the phenotype-genotype relationships in PSC synthetase deficiency in higher vertebrates.

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“…Variability in freezing tolerances in the progeny of DH plants obtained from a single mother plant (OP22/1, OP22/2 and CH993/1, 2, 3, 4) was observed (Table 3). Many previous studies that dealt with the problems of cold stress showed a positive correlation between the accumulated proline in plant tissues or cell cultures and the plant's freezing tolerance, particularly in cereals (Charest & Phan 1990;Dörffling et al 1993Dörffling et al , 1994Dörffling et al , 1997aChen & Li 2002;Tantau et al 2004). However, in yet other studies this relationship was not so explicit (e.g.…”

mentioning

confidence: 96%

“…Selection for improved freezing tolerance with Hyp was successfully used for callus cultures of potato (Van Swaaij et al 1987;Anjum & Villiers 1998) and for both the cell lines and the embryogenic calluses of cereals (Dörffling et al 1993(Dörffling et al , 1997aTantau et al 2004). Recently, McClinchey and Kott (2008) successfully used combined UV mutagenesis and in vitro selection in microspore cultures with several proline analogues for the improvement of freezing tolerance in Canadian spring oilseed rape.…”

mentioning

confidence: 99%

“…Many studies have shown a positive correlation between the resistance of tissue cultures to Hyp and the content of proline in their tissues, particularly in cereals (Tantau & Dörffling 1991;Dörffling et al 1993Dörffling et al , 1994Dörffling et al , 1997Tantau et al 2004) and in potatoes (Van Swaaij et al 1987;Anjum & Villiers 1998). In contrast, there are also studies in which this correlation was not confirmed (e.g.…”

mentioning

confidence: 99%

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Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape

Janská¹,

Zelenková²,

Klíma³

et al. 2010

CZECH J GENET PLANT

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Twelve doubled haploid (DH) winter oilseed rape plants with altered levels of proline and/or freezing tolerance were obtained by in vitro selection for resistance to trans-4-hydroxy-l-proline (Hyp) in five segregating microspore populations. No significant response to selection either in proline content or in freezing tolerance, compared with the non-selected control populations, was observed. When data from all examined materials were combined, a weak correlation between proline content and freezing tolerance was observed.

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In vitro-Selection and Regeneration of Hydroxyproline-Resistant Lines of Winter Wheat with Increased Proline Content and Increased Frost Tolerance

Cited by 51 publications

References 9 publications

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

The proline biosynthesis in living organisms

Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape

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