In vitro selection of elite clone of Withania somnifera against leaf blight disease caused by Alternaria alternata

Chakraborty, Nilanjan; Banerjee, Maitreyi; Acharya, Krishnendu

doi:10.1016/j.pmpp.2020.101560

Cited by 9 publications

(1 citation statement)

References 42 publications

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“…Thus far, there is no information about the addition of ACT to media in citrus shoots in previous reports, and it is also scarce in other species. Only Chakraborty et al (2020) [ 13 ] reported induced-tolerance in shoots of Withania somnifera to A. alternata by exposing calli to a media containing mycotoxin isolated from an A. alternata culture. The differences between this study and the current trial can be found in the concentration of the toxin and the sensitivity of certain tissues and species to the toxin.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Four Systems to Test the Tolerance of ‘Fortune’ Mandarin Tissue Cultured Plants to Alternaria alternata

Pérez‐Jiménez

Pérez‐Tornero

2021

Plants

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Alternaria brown spot is a severe disease that affects leaves and fruits on susceptible mandarin and mandarin-like cultivars, and is produced by Alternaria alternata. Consequently, there is an urge to obtain new cultivars resistant to A. alternata, and mutation breeding together with tissue culture can help shorten the process. However, a protocol for the in vitro selection of resistant citrus genotypes is lacking. In this study, four methods to evaluate the sensitivity to Alternaria of mandarin ‘Fortune’ explants in in vitro culture were tested. The four tested systems consisted of: (1) the addition of the mycotoxin, produced by A. alternata in ‘Fortune’, to the propagation culture media, (2) the addition of the A. alternata culture filtrate to the propagation culture media, (3) the application of the mycotoxin to the intact shoot leaves, and (4) the application of the mycotoxin to the previously excised and wounded leaves. After analyzing the results, only the addition of the A. alternata culture filtrate to the culture media and the application of the mycotoxin to the wounded leaves produced symptoms of infection. However, the addition of the fungus culture filtrate to the culture media produced results, which might indicate that, in addition to the mycotoxin, many other unknown elements that can affect the plant growth and behavior could be found in the fungus culture filtrate. Therefore, the application of the toxin to the excised and wounded leaves seems to be the most reliable method to analyze sensitivity to Alternaria of ‘Fortune’ explants cultured in vitro.

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Section: Discussionmentioning

confidence: 99%

Comparison of Four Systems to Test the Tolerance of ‘Fortune’ Mandarin Tissue Cultured Plants to Alternaria alternata

Pérez‐Jiménez

Pérez‐Tornero

2021

Plants

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Somaclonal Variation in Improvement of Agricultural Crops: Recent Progress

Manoj

2021

Agricultural Biotechnology: Latest Research and Trends

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In vitro selection, regeneration and characterization of NaCl-tolerant plants of Sapindus trifoliatus: an important multipurpose tree

Asthana

Manoj

Jaiswal

2023

Plant Cell Tiss Organ Cult

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The present study demonstrates the selection of NaCl tolerant callus line and regeneration of NaCl tolerant plants of Sapindus trifoliatus L. from selected callus via somatic embryogenesis. Callus induced from leaf disc explants was subjected to the selection of NaCl-tolerant callus line on MS medium containing NaCl. The exposure of embryogenic callus to NaCl (0-250 mM) allowed the selection of callus to tolerate 100 mM NaCl, and it con rms with stability test by the three consecutive transfers of 100 mM NaCl tolerant callus to a medium devoid of NaCl and re-transfer to 100 mM NaCl supplemented medium.NaCl selected and non-selected embryogenic callus lines were characterized by the analysis of callus growth, tolerance index, and accumulation of both inorganic (Na + and K + ) and organic (proline, glycine betaine and soluble sugars) content. 100mM NaCl-tolerant callus showed the formation of globular structure, which differentiated different stages of somatic embryos on a maintenance medium containing NaCl (0-200mM). Mature cotyledonary stage somatic embryos developed from 100 mM NaCl tolerant globular structure were germinated on germination medium containing NaCl. About 67 and 38% NaCl tolerant somatic embryos were germinated on a medium without NaCl and containing 100 mM NaCl, respectively. NaCl tolerant plants developed on different germination medium with NaCl survived when transplanted to 0.2 or 0.3% (w/w) NaCl mixed potting mixture. Six-month-old established 100 mM NaCl tolerant plants were characterized by analyzing various physiological and biochemical parameters i.e., chlorophyll, carotenoid, L-proline content and antioxidative (MDA, SOD, CAT and APx) activities. The results suggest that in vitro selection technique offers an e cient way to develop NaCl tolerant plants of S. trifoliatus.

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In vitro selection of elite clone of Withania somnifera against leaf blight disease caused by Alternaria alternata

Cited by 9 publications

References 42 publications

Comparison of Four Systems to Test the Tolerance of ‘Fortune’ Mandarin Tissue Cultured Plants to Alternaria alternata

Comparison of Four Systems to Test the Tolerance of ‘Fortune’ Mandarin Tissue Cultured Plants to Alternaria alternata

Somaclonal Variation in Improvement of Agricultural Crops: Recent Progress

In vitro selection, regeneration and characterization of NaCl-tolerant plants of Sapindus trifoliatus: an important multipurpose tree

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