In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd

Abstract

Multiple shoot buds could be induced directly from internode explants of Celastrus paniculatus inoculated on Murashige and Skoog's (MS) medium containing different growth regulators. The best response was obtained when 4.44 µM 6-benzylaminopurine (BAP) was incorporated in the medium. Incorporation of indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) did not improve response, rather promoted callusing. Adventitious shoot buds could be multiplied and elongated on MS medium containing 2.22 µM BAP. Rooting of shoots (80 %) was obtained when their bases were dipped in pre-autoclaved indole-3-butyric acid (IBA) solution (2.45 mM) for 10 min followed by their implantation on medium containing ¼ MS salts, 1.0 % sucrose and 0.6 % agar. Out of 500 plantlets subjected to hardening, 410 were successfully hardened under greenhouse conditions. Twenty plants were established in field while remaining of them were transferred to nursery conditions without any mortality.

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

“…In this paper a micropropagation protocol for mass production was developed and molecular markers were used to assess the genetic stability of micropropagated C. paniculatus plants. There are few records on the micropropagation of this valuable species through callus (Sharada et al 2003) and through bud differentiation (Rao & Purohit 2006;Lal & Singh 2010). In this report, the effect of various concentrations of auxins (NAA, IBA and IAA) and cytokinins (BAP and Kn) were analyzed for high frequency micropropagation through direct organogenesis from intact nodal explants of C. paniculatus.…”

Establishment of an Improved, Efficient and Eco-Friendly Micropropagation System in Salacia Chinensis L. An Endangered Anti-Diabetic Medicinal Plant

“…BAP is structurally stable and the plant cells ability to its easy assimilation makes this kind of cytokinin stand out among the others (Ahmad et al, 2013). BAP strong promotive effects on shoot regeneration were reported previously (Rao and Purohit, 2006;Mozafari et al, 2015). Suitable individual concentration of BAP was 1.5 mg/l for shoot induction in node, while this rate was 2.0 mg/l for Kin.…”

Development of a Successful Protocol for <i>in vitro</i> Mass Propagation of <i>Celastrus paniculatus</i> Willd. – A Valuable Medicinal Plant

“…MS (Murashige and Skoog, 1962) medium was used (Sharada et al, 2003, Rao andPurohit, 2006) as the basal medium. Based on the results of previous experiments, BAP (Benzyl amino purine) (5.0 -10.0 µM) and IAA (Indole-3-acetic acid) (7.0 -14.0 µM) were added in different combinations to the culture media.…”

“…Rooting of cuttings is also not successful. There are some records on the microporpagation of this valuable species through callus (Sharada et al, 2003) and through bud differentiation (Rao and Purohit, 2006) with limited success. Therefore, an attempt was made to mass propagate C. paniculatus through tissue culture techniques and to establish tissue cultured plants in the greenhouse successfully.…”